Gene/Proteome Database (LMPD)

LMPD ID
LMP007276
Gene ID
855392
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
phosphatidylinositol-3,5-bisphosphate 5-phosphatase
Gene Symbol
FIG4
Synonyms
-
Alternate Names
phosphatidylinositol-3,5-bisphosphate 5-phosphatase
Chromosome
XIV
EC Number
3.1.3.-

Proteins

phosphatidylinositol-3,5-bisphosphate 5-phosphatase
Refseq ID NP_014074
Protein GI 6324005
UniProt ID P42837
mRNA ID NM_001183163
Length 879
RefSeq Status PROVISIONAL
MNNDAMEHTLGGGILTTSGSKQRKTSKFVMGKYTLYETKDRMYIVGSNKRETMFRILEIDLTVPRGELTVLEDNVFFTRNEIMNVLASLEEATEDGLHKKITGYGLLGFIKFTCWYYLIMVTKYSQVAVIGGHGIYHIDGIDIIPITNNYKKPEKSSDEARLLNIFKDLDLTKTFYFSYTYDITNTLQTNILREKLKAVDRCDITIPCGITDYNEMFVWNNNLLSPIFACIDTVFDWFQCIIHGFIDQVNVSVLGKSIYITLIARRSHHFAGARFLKRGVNNKGHVANEVETEQIVTDMILTPFHQPGNGFFDSDRYTSFVQHRGSIPLYWTQDASNLTTKPPIRINVVDPFFSPAALHFDNLFQRYGGGTIQILNLIKTKEKTPRETKLLWEFEQCIDYLNEFLPTLKKLDYTSWDMSRASKQDGQGVIEFLEKYAVNTVTTTGIFHNGPDFASTKIQEGICRSNCIDCLDRTNAAQFVIGKRALGCQLKSLGIIDNSYLEYDSDIVNILTELFHDLGDTIALQYGGSHLVNTMETYRKINQWSSHSRDMIESIKRFYSNSFVDAQRQDAINLFLGHYSWREGFPSLWEMNTDFYLHNAYSLNMPKRSYIHWWNDYNIKSVKELINEELIATGNDVTREKIIKNVRGYPGAFDNYWNEYYLPRSVTWIRDLFAYNMNSTRRYHNALSKQDKAMSPFTSRKQSWLNNKLKMITSSKSLEKAEGRVVETTDLDRDTSPKQELELYEHYLHIISDRSQKLEEKMNSFSYSKYPIFISHESSEIPPMRKVIGEPLVDIAEDFTDVYDDDDDGDDENDEMTTEALLIAPDHVSVDEKFYEKVLNVDDYKPALDDYSAVIHIKPDNLQLYRDLCFSKDIQLDFQ

Gene Information

Entrez Gene ID
855392
Gene Name
phosphatidylinositol-3,5-bisphosphate 5-phosphatase
Gene Symbol
FIG4
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0070772 IDA:SGD C PAS complex
GO:0019898 IDA:SGD C extrinsic component of membrane
GO:0000329 IDA:SGD C fungal-type vacuole membrane
GO:0043813 IDA:SGD F phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity
GO:0046856 IDA:SGD P phosphatidylinositol dephosphorylation
GO:0036092 IDA:GOC P phosphatidylinositol-3-phosphate biosynthetic process

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY3O-242 phosphatidylinositol phosphate biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5618614 Synthesis of PIPs at the Golgi membrane
5618615 Synthesis of PIPs at the early endosome membrane
5618612 Synthesis of PIPs at the late endosome membrane

Domain Information

InterPro Annotations

Accession Description
IPR002013 SAC domain

UniProt Annotations

Entry Information

Gene Name
phosphatidylinositol-3,5-bisphosphate 5-phosphatase
Protein Entry
FIG4_YEAST
UniProt ID
P42837
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 3,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate. {ECO:0000269|PubMed:14528018}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:14528018};
Function The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Major enzyme required for hyperosmotic shock- induced turnover of PtdIns(3,5)P2 and requires VAC14 for this function. In vivo, mediates turnover of PtdIns(3,5)P2 at the vacuole membrane necessary for vacuolar size control. In vitro, catalyzes the removal of phosphate from the fifth hydroxyl of the myo-inositol ring of phosphatidylinositol 3,5-bisphosphate. {ECO:0000269|PubMed:11950935, ECO:0000269|PubMed:14528018, ECO:0000269|PubMed:16492811, ECO:0000269|PubMed:19037259}.
Interaction P34756:FAB1; NbExp=4; IntAct=EBI-28407, EBI-6754; Q06708:VAC14; NbExp=7; IntAct=EBI-28407, EBI-27189;
Miscellaneous Present with 339 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Similarity Contains 1 SAC domain. {ECO:0000255|PROSITE- ProRule:PRU00183}.
Subcellular Location Vacuole membrane {ECO:0000269|PubMed:11950935, ECO:0000269|PubMed:14528018, ECO:0000269|PubMed:18653468, ECO:0000269|PubMed:19037259}; Peripheral membrane protein {ECO:0000269|PubMed:11950935, ECO:0000269|PubMed:14528018, ECO:0000269|PubMed:18653468, ECO:0000269|PubMed:19037259}. Note=Localized to the limiting membrane of the vacuole. Localization requires VAC14 and FAB1.
Subunit Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold. {ECO:0000269|PubMed:18653468, ECO:0000269|PubMed:19037259}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007276 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6324005 RefSeq NP_014074 879 phosphatidylinositol-3,5-bisphosphate 5-phosphatase

Identical Sequences to LMP007276 proteins

Reference Database Accession Length Protein Name
GI:6324005 EMBL CAA86373.1 879 NO330 [Saccharomyces cerevisiae]
GI:6324005 EMBL CAA96256.1 879 unnamed protein product [Saccharomyces cerevisiae]
GI:6324005 GenBank EIW08198.1 879 Fig4p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6324005 SwissProt P42837.1 879 RecName: Full=Polyphosphoinositide phosphatase; AltName: Full=Factor-induced gene 4 protein; AltName: Full=Phosphatidylinositol 3,5-bisphosphate 5-phosphatase [Saccharomyces cerevisiae S288c]
GI:6324005 Third Party Genbank DAA10238.1 879 TPA: phosphatidylinositol-3,5-bisphosphate 5-phosphatase [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007276 proteins

Reference Database Accession Length Protein Name
GI:6324005 DBBJ GAA25788.1 879 K7_Fig4p [Saccharomyces cerevisiae Kyokai no. 7]
GI:6324005 EMBL CAY82289.1 881 Fig4p [Saccharomyces cerevisiae EC1118]
GI:6324005 GenBank EDN62505.1 879 conserved protein [Saccharomyces cerevisiae YJM789]
GI:6324005 GenBank EWG88961.1 881 Fig4p [Saccharomyces cerevisiae P301]
GI:6324005 GenBank EWG93859.1 881 Fig4p [Saccharomyces cerevisiae R103]
GI:6324005 GenBank EWH16315.1 881 Fig4p [Saccharomyces cerevisiae P283]