Gene/Proteome Database (LMPD)

LMPD ID
LMP007285
Gene ID
854233
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase
Gene Symbol
ALG8
Synonyms
YOR29-18
Chromosome
XV
EC Number
2.4.1.265

Proteins

dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase
Refseq ID NP_014710
Protein GI 6324641
UniProt ID P40351
mRNA ID NM_001183486
Length 577
MKGDRSRQNMAVTKKAKLKKNDEPKKVLKTAATEKGEGSKRYSLWNFWISTLFLKLLLIPDYFSTDFDVHRNWLAITNKLPISEWYYEHTSQWTLDYPPFFAYFEWFLSQFVPKSVRDDGCLDIVEIGKFGLPTIVFQRLTVIFSEILLFVILQIYINTTKLSERSQSFVVASSIVLSPGFLIIDHIHFQYNGFLFAILIGSIVAAKNKRYILCAVLYTTAICFKHIFLYLAPCYFVFLLRAYVLNVNNFKFKSYKDFLFLIRWANLLKLATVVVGIFTICFLPFAHQMPQVLSRLFPFSRGLTHAYWAPNFWALYSFMDKILTTVMLKLPYVHTFATKFIKPPLIPQNIKEINERLAANNNGSKGLVQDVFFVILPQIPPKLTFILTIFYQVLAVLPLLFDPSFKRFVGSLTLCGLASFLFGWHVHEKAIMLVIIPFTFLVGFDRRLLVPFMLVASAGYVSLYPLLYKGQDFFIKTLYTYVWCIIYFAAFRKTTKISSSVERRIFFLDRLALTYIFSLLPIVTVLQILDEVKWRYSFLQKFEFLGLMIYSVYCSLGIISSWFALSWLYNFDELLWQ

Gene Information

Entrez Gene ID
854233
Gene Name
dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase
Gene Symbol
ALG8
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005789 IMP:SGD C endoplasmic reticulum membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0042283 IMP:SGD F dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity
GO:0006490 IMP:SGD P oligosaccharide-lipid intermediate biosynthetic process
GO:0006487 IGI:SGD P protein N-linked glycosylation

KEGG Pathway Links

KEGG Pathway ID Description
sce01100 Metabolic pathways
ko00510 N-Glycan biosynthesis
sce00510 N-Glycan biosynthesis
M00055 N-glycan precursor biosynthesis
sce_M00055 N-glycan precursor biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID Description
MANNOSYL-CHITO-DOLICHOL-BIOSYNTHESIS dolichyl-diphosphooligosaccharide biosynthesis
MANNOSYL-CHITO-DOLICHOL-BIOSYNTHESIS dolichyl-diphosphooligosaccharide biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5618350 Asparagine N-linked glycosylation
5618349 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
5618074 Metabolism of proteins
5618285 Post-translational protein modification

Domain Information

InterPro Annotations

Accession Description
IPR004856 Glycosyl transferase, ALG6/ALG8

UniProt Annotations

Entry Information

Gene Name
dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase
Protein Entry
ALG8_YEAST
UniProt ID
P40351
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity Dolichyl beta-D-glucosyl phosphate + D-Glc- alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha- (1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)- D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D- GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha- (1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D- Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man- alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta- (1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate
Catalytic Activity Dolichyl beta-D-glucosyl phosphate + D-Glc- alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha- (1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)- D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D- GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha- (1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D- Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man- alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta- (1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. {ECO:0000269|PubMed:3536907}.
Function Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(1)Man(9)GlcNAc(2)-PP-Dol
Function Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(1)Man(9)GlcNAc(2)-PP-Dol. {ECO:0000269|PubMed:3536907}.
Pathway Protein modification; protein glycosylation.
Similarity Belongs to the ALG6/ALG8 glucosyltransferase family
Similarity Belongs to the ALG6/ALG8 glucosyltransferase family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein.

Identical and Related Proteins

Unique RefSeq proteins for LMP007285 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6324641 RefSeq NP_014710 577 dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase

Identical Sequences to LMP007285 proteins

Reference Database Accession Length Protein Name

Related Sequences to LMP007285 proteins

Reference Database Accession Length Protein Name