Gene/Proteome Database (LMPD)
Proteins
| phosphoprotein phosphatase PP4 catalytic subunit PPH3 | |
|---|---|
| Refseq ID | NP_010360 |
| Protein GI | 6320280 |
| UniProt ID | P32345 |
| mRNA ID | NM_001180383 |
| Length | 308 |
| MMDLDKIIASLRDGKHIPEETVFRLCLNSQELLMNEGNVTQVDTPVTICGDIHGQLHDLLTLFEKSGGVEKTRYIFLGDFVDRGFYSLESFLLLLCYKLRYPDRITLIRGNHETRQITKVYGFYDEVVRKYGNSNVWRYCCEVFDYLSLGAIINNSIFCVHGGLSPDMTTVDEIRTIDRKQEVPHEGAMCDLLWSDPEDVDTWSLSPRGAGFLFGKREVDQFLEKNNVELIARAHQLVMEGYKEMFDGGLVTVWSAPNYCYRCGNVAAVLKIDDDLNREYTIFEAVQAQNEVGNAIIPTKKSQMDYFL | |
Gene Information
Entrez Gene ID
Gene Name
phosphoprotein phosphatase PP4 catalytic subunit PPH3
Gene Symbol
Species
Saccharomyces cerevisiae S288c
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IEA:UniProtKB-KW | C | cytoplasm |
| GO:0005634 | IEA:UniProtKB-KW | C | nucleus |
| GO:0030289 | IDA:SGD | C | protein phosphatase 4 complex |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0004722 | IDA:SGD | F | protein serine/threonine phosphatase activity |
| GO:0000724 | IGI:SGD | P | double-strand break repair via homologous recombination |
| GO:2000002 | IMP:SGD | P | negative regulation of DNA damage checkpoint |
| GO:1902660 | IMP:SGD | P | negative regulation of glucose mediated signaling pathway |
| GO:2001034 | IMP:SGD | P | positive regulation of double-strand break repair via nonhomologous end joining |
| GO:0051173 | IMP:SGD | P | positive regulation of nitrogen compound metabolic process |
| GO:0006470 | IDA:SGD | P | protein dephosphorylation |
| GO:0072462 | IMP:SGD | P | signal transduction involved in meiotic recombination checkpoint |
Domain Information
UniProt Annotations
Entry Information
Gene Name
phosphoprotein phosphatase PP4 catalytic subunit PPH3
Protein Entry
PP4C_YEAST
UniProt ID
Species
Yeast (S288c)
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | [a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate. |
| Cofactor | Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence= ; Note=Binds 2 manganese ions per subunit. ; |
| Cofactor | Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Binds 2 manganese ions per subunit. {ECO:0000250}; |
| Function | Forms the histone H2A phosphatase complex in association with the regulatory subunits PSY2 and PSY4, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. PPH3 is directly involved in the dephosphorylation and activation of the transcription factor GLN3 in response to nutrient availability |
| Function | Forms the histone H2A phosphatase complex in association with the regulatory subunits PSY2 and PSY4, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. PPH3 is directly involved in the dephosphorylation and activation of the transcription factor GLN3 in response to nutrient availability. {ECO:0000269|PubMed:10940301, ECO:0000269|PubMed:16299494}. |
| Interaction | P38193:PSY4; NbExp=8; IntAct=EBI-12759, EBI-21239; |
| Miscellaneous | Present with 2840 molecules/cell in log phase SD medium |
| Miscellaneous | Present with 2840 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Ptm | Reversibly methyl esterified on Leu-308 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization (By similarity) |
| Ptm | Reversibly methyl esterified on Leu-308 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization (By similarity). {ECO:0000250}. |
| Similarity | Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily |
| Similarity | Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily. {ECO:0000305}. |
| Subcellular Location | Cytoplasm . Nucleus . |
| Subcellular Location | Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}. |
| Subunit | Catalytic subunit of the histone H2A phosphatase complex (HTP-C) containing PPH3, PSY2 and PSY4. Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Interacts with SPT5 and TAP42. {ECO:0000269|PubMed:14551259, ECO:0000269|PubMed:15447631, ECO:0000269|PubMed:16085932, ECO:0000269|PubMed:16299494}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007387 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 6320280 | RefSeq | NP_010360 | 308 | phosphoprotein phosphatase PP4 catalytic subunit PPH3 |
Identical Sequences to LMP007387 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|
Related Sequences to LMP007387 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|