Gene/Proteome Database (LMPD)

LMPD ID
LMP007487
Gene ID
851210
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
dolichyl-phosphate-mannose-protein mannosyltransferase PMT2
Gene Symbol
PMT2
Synonyms
FUN25
Alternate Names
dolichyl-phosphate-mannose-protein mannosyltransferase PMT2
Chromosome
I
EC Number
2.4.1.109

Proteins

dolichyl-phosphate-mannose-protein mannosyltransferase PMT2
Refseq ID NP_009379
Protein GI 27469358
UniProt ID P31382
mRNA ID NM_001178168
Length 759
RefSeq Status PROVISIONAL
MSSSSSTGYSKNNAAHIKQENTLRQRESSSISVSEELSSADERDAEDFSKEKPAAQSSLLRLESVVMPVIFTALALFTRMYKIGINNHVVWDEAHFGKFGSYYLRHEFYHDVHPPLGKMLVGLSGYLAGYNGSWDFPSGEIYPDYLDYVKMRLFNASFSALCVPLAYFTAKAIGFSLPTVWLMTVLVLFENSYSTLGRFILLDSMLLFFTVASFFSFVMFHNQRSKPFSRKWWKWLLITGISLGCTISVKMVGLFIITMVGIYTVIDLWTFLADKSMSWKTYINHWLARIFGLIIVPFCIFLLCFKIHFDLLSHSGTGDANMPSLFQARLVGSDVGQGPRDIALGSSVVSIKNQALGGSLLHSHIQTYPDGSNQQQVTCYGYKDANNEWFFNRERGLPSWSENETDIEYLKPGTSYRLVHKSTGRNLHTHPVAAPVSKTQWEVSGYGDNVVGDNKDNWVIEIMDQRGDEDPEKLHTLTTSFRIKNLEMGCYLAQTGNSLPEWGFRQQEVVCMKNPFKRDKRTWWNIETHENERLPPRPEDFQYPKTNFLKDFIHLNLAMMATNNALVPDPDKFDYLASSAWQWPTLNVGLRLCGWGDDNPKYFLLGTPASTWASSVAVLAFMATVVILLIRWQRQYVDLRNPSNWNVFLMGGFYPLLAWGLHYMPFVIMSRVTYVHHYLPALYFALIILAYCFDAGLQKWSRSKCGRIMRFVLYAGFMALVIGCFWYFSPISFGMEGPSSNFRYLNWFSTWDIADKQEA

Gene Information

Entrez Gene ID
851210
Gene Name
dolichyl-phosphate-mannose-protein mannosyltransferase PMT2
Gene Symbol
PMT2
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0097582 IDA:SGD C dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt2p dimer complex
GO:0097584 IDA:SGD C dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt2p dimer complex
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0004169 IDA:SGD F dolichyl-phosphate-mannose-protein mannosyltransferase activity
GO:0071712 IMP:SGD P ER-associated misfolded protein catabolic process
GO:0006493 IMP:SGD P protein O-linked glycosylation
GO:0035269 IMP:SGD P protein O-linked mannosylation
GO:0032527 IGI:SGD P protein exit from endoplasmic reticulum
GO:1900101 IMP:SGD P regulation of endoplasmic reticulum unfolded protein response

KEGG Pathway Links

KEGG Pathway ID Description
ko00514 Other types of O-glycan biosynthesis
sce00514 Other types of O-glycan biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR003342 Glycosyl transferase, family 39
IPR027005 Glycosyltransferase 39 like
IPR016093 MIR_motif

UniProt Annotations

Entry Information

Gene Name
dolichyl-phosphate-mannose-protein mannosyltransferase PMT2
Protein Entry
PMT2_YEAST
UniProt ID
P31382
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity Dolichyl D-mannosyl phosphate + protein = dolichyl phosphate + O-D-mannosylprotein. {ECO:0000269|PubMed:8543034}.
Disruption Phenotype Leads to diminished in vitro and in vivo O- mannosylation activity. {ECO:0000269|PubMed:7852348, ECO:0000269|PubMed:8918452}.
Function Protein O-mannosyltransferase involved in O- glycosylation which is essential for cell wall rigidity. Forms a heterodimeric complex with PMT2 and more rarely with PMT5 to transfer mannose from Dol-P-mannose to Ser or Thr residues on proteins. The PMT1-PMT2 complex participates to oxidative protein folding, ER-associated protein degradation (ERAD), as well as ER export. {ECO:0000269|PubMed:15377669, ECO:0000269|PubMed:18182384, ECO:0000269|PubMed:21147851, ECO:0000269|PubMed:8543034}.
Interaction P33775:PMT1; NbExp=4; IntAct=EBI-13573, EBI-13567; P52867:PMT5; NbExp=2; IntAct=EBI-13573, EBI-13591;
Miscellaneous Present with 6510 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Protein modification; protein glycosylation. {ECO:0000305}.
Similarity Belongs to the glycosyltransferase 39 family. {ECO:0000305}.
Similarity Contains 3 MIR domains. {ECO:0000255|PROSITE- ProRule:PRU00131}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P33775}; Multi-pass membrane protein {ECO:0000255}.
Subunit PMT1 and PMT2 form a functional heterodimer. The complex interacts with endoplasmic reticulum proteins EMP24, ERV25, ERP1, ERP2, CDC48, HRD1, USA1, YOS9, ERO1, PDI1, UBR1, Cue4, DFM1 and TED1. Forms also a minor complex with PMT5. {ECO:0000269|PubMed:12551906, ECO:0000269|PubMed:21147851, ECO:0000269|PubMed:8543034}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007487 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
27469358 RefSeq NP_009379 759 dolichyl-phosphate-mannose-protein mannosyltransferase PMT2

Identical Sequences to LMP007487 proteins

Reference Database Accession Length Protein Name
GI:27469358 GenBank EGA84105.1 759 Pmt2p [Saccharomyces cerevisiae Lalvin QA23]
GI:27469358 GenBank EHN08700.1 759 Pmt2p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7]
GI:27469358 GenBank EIW12340.1 759 Pmt2p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:27469358 GenBank EWG92662.1 759 Pmt2p [Saccharomyces cerevisiae P301]
GI:27469358 GenBank EWG97708.1 759 Pmt2p [Saccharomyces cerevisiae R103]
GI:27469358 gnl McCuskerlabDuke 759 Pmt2p [Saccharomyces cerevisiae YJM993]

Related Sequences to LMP007487 proteins

Reference Database Accession Length Protein Name
GI:27469358 EMBL CAR22385.1 740 KLTH0D02266p [Lachancea thermotolerans CBS 6340]
GI:27469358 EMBL CCD26428.1 757 hypothetical protein NDAI_0H02540 [Naumovozyma dairenensis CBS 421]
GI:27469358 EMBL CCE94234.1 750 hypothetical protein TDEL_0H03750 [Torulaspora delbrueckii]
GI:27469358 GenBank EWH19707.1 759 Pmt2p [Saccharomyces cerevisiae P283]
GI:27469358 RefSeq XP_002552823.1 740 KLTH0D02266p [Lachancea thermotolerans CBS 6340]
GI:27469358 RefSeq XP_003683445.1 750 hypothetical protein TDEL_0H03750 [Torulaspora delbrueckii]