Gene/Proteome Database (LMPD)

LMPD ID
LMP007544
Gene ID
949134
Species
Escherichia coli K-12 (E. coli)
Gene Name
D-sedoheptulose 7-phosphate isomerase
Gene Symbol
gmhA
Synonyms
ECK0223; JW0212; isn; lpcA; tfrA; yafI
Summary
Sedoheptulose 7-phosphate isomerase catalyzes the first committed step in the biosynthesis of a core component of lipopolysaccharide, the isomerization of D-sedoheptulose 7-phosphate to D-glycero-D-manno-heptose 7-phosphate. [More information is available at EcoCyc: G6106].
Orthologs
View orthologs and multiple alignments for gmhA

Proteins

D-sedoheptulose 7-phosphate isomerase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_414757
Protein GI 16128208
UniProt ID P63224
Length 192
RefSeq Status REVIEWED
MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAGGKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFSRYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEIRVPHFGYADRIQEIHIKVIHILIQLIEKEMVK

Gene Information

Entrez Gene ID
949134
Gene Name
D-sedoheptulose 7-phosphate isomerase
Gene Symbol
gmhA
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 ISS:EcoCyc C cytoplasm
GO:0008968 IDA:EcoCyc F D-sedoheptulose 7-phosphate isomerase activity
GO:0030246 IEA:InterPro F carbohydrate binding
GO:0008270 IEA:UniProtKB-HAMAP F zinc ion binding
GO:2001061 IEA:UniProtKB-UniPathway P D-glycero-D-manno-heptose 7-phosphate biosynthetic process
GO:0009244 IMP:EcoCyc P lipopolysaccharide core region biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
M00064 ADP-L-glycero-D-manno-heptose biosynthesis
eco_M00064 ADP-L-glycero-D-manno-heptose biosynthesis
eco00540 Lipopolysaccharide biosynthesis
ko00540 Lipopolysaccharide biosynthesis
eco01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY0-1241 ADP-L-glycero-beta-D-manno-heptose biosynthesis
PWY0-1241 ADP-L-glycero-beta-D-manno-heptose biosynthesis
PWY0-1241 ADP-L-glycero-beta-D-manno-heptose biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR004515 Phosphoheptose_Isoase
IPR001347 Sugar isomerase (SIS)

UniProt Annotations

Entry Information

Gene Name
D-sedoheptulose 7-phosphate isomerase
Protein Entry
GMHA_ECOLI
UniProt ID
P63224
Species
E. coli

Comments

Comment Type Description
Catalytic Activity D-sedoheptulose 7-phosphate = D-glycero-D- manno-heptose 7-phosphate. {ECO:0000269|PubMed:18056714}.
Cofactor Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250}; Note=Binds 1 zinc ion per subunit. {ECO:0000250};
Function Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate. {ECO:0000269|PubMed:11751812, ECO:0000269|PubMed:18056714}.
Miscellaneous The reaction produces a racemic mixture of D- glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D- manno-heptose 7-phosphate.
Pathway Bacterial outer membrane biogenesis; LPS core biosynthesis.
Pathway Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7- phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7- phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1.
Sequence Caution Sequence=AAB08644.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
Similarity Belongs to the SIS family. GmhA subfamily. {ECO:0000305}.
Similarity Contains 1 SIS domain. {ECO:0000305}.
Subcellular Location Cytoplasm.
Subunit Homotetramer. {ECO:0000269|PubMed:18056714}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007544 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16128208 RefSeq NP_414757 192 D-sedoheptulose 7-phosphate isomerase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007544 proteins

Reference Database Accession Length Protein Name
GI:16128208 GenBank KHJ04766.1 192 phosphoheptose isomerase [Escherichia coli]
GI:16128208 GenBank KHJ07921.1 192 phosphoheptose isomerase [Escherichia coli]
GI:16128208 GenBank KHJ17430.1 192 phosphoheptose isomerase [Escherichia coli]
GI:16128208 GenBank KHJ23973.1 192 phosphoheptose isomerase [Escherichia coli]
GI:16128208 GenBank KHJ27415.1 192 phosphoheptose isomerase [Escherichia coli]
GI:16128208 gnl IGS 192 D-sedoheptulose 7-phosphate isomerase [Escherichia coli ER2796]

Related Sequences to LMP007544 proteins

Reference Database Accession Length Protein Name
GI:16128208 GenBank EGI17676.1 246 sedoheptulose 7-phosphate isomerase [Escherichia coli M605]
GI:16128208 GenBank EGI22946.1 246 sedoheptulose 7-phosphate isomerase [Escherichia coli M718]
GI:16128208 GenBank EGI47742.1 246 sedoheptulose 7-phosphate isomerase [Escherichia coli H591]
GI:16128208 GenBank EGJ07057.1 246 phosphoheptose isomerase [Escherichia coli D9]
GI:16128208 gnl ChinaCDC 246 Phosphoheptose isomerase [Shigella flexneri 2002017]
GI:16128208 RefSeq WP_000335812.1 246 MULTISPECIES: phosphoheptose isomerase [Enterobacteriaceae]