Gene/Proteome Database (LMPD)

LMPD ID
LMP007569
Gene ID
945774
Species
Escherichia coli K-12 (E. coli)
Gene Name
4-diphosphocytidyl-2-C-methylerythritol kinase
Gene Symbol
ispE
Synonyms
ECK1196; JW1199; ipk; ychB
Summary
[More information is available at EcoCyc: EG11294].
Orthologs
View orthologs and multiple alignments for ispE

Proteins

4-diphosphocytidyl-2-C-methylerythritol kinase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_415726
Protein GI 16129171
UniProt ID P62615
Length 283
RefSeq Status REVIEWED
MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIELRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGGGLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEILTPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKRFREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLSPLHRAML

Gene Information

Entrez Gene ID
945774
Gene Name
4-diphosphocytidyl-2-C-methylerythritol kinase
Gene Symbol
ispE
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0050515 IDA:EcoCyc F 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity
GO:0005524 IDA:EcoCyc F ATP binding
GO:0019288 IEA:UniProtKB-UniPathway P isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
GO:0016114 IEA:UniProtKB-HAMAP P terpenoid biosynthetic process
GO:0006744 IDA:EcoCyc P ubiquinone biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco01110 Biosynthesis of secondary metabolites
M00096 C5 isoprenoid biosynthesis, non-mevalonate pathway
eco_M00096 C5 isoprenoid biosynthesis, non-mevalonate pathway
eco01100 Metabolic pathways
eco00900 Terpenoid backbone biosynthesis
ko00900 Terpenoid backbone biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID Description
NONMEVIPP-PWY methylerythritol phosphate pathway
NONMEVIPP-PWY methylerythritol phosphate pathway
PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PWY-7392 taxadiene biosynthesis (engineered)

Domain Information

InterPro Annotations

Accession Description
IPR004424 4-diphosphocytidyl-2C-methyl-D-erythritol kinase
IPR006204 GHMP kinase N-terminal domain
IPR013750 GHMP kinase, C-terminal domain
IPR020568 Ribosomal protein S5 domain 2-type fold
IPR014721 Ribosomal protein S5 domain 2-type fold, subgroup

UniProt Annotations

Entry Information

Gene Name
4-diphosphocytidyl-2-C-methylerythritol kinase
Protein Entry
ISPE_ECOLI
UniProt ID
P62615
Species
E. coli

Comments

Comment Type Description
Catalytic Activity ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D- erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl- D-erythritol.
Caution Was originally thought to be an isopentenyl monophosphate kinase. {ECO:0000305|PubMed:1427085}.
Function Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol. Phosphorylates isopentenyl phosphate at low rates. Also acts on isopentenol, and, much less efficiently, dimethylallyl alcohol. Dimethylallyl monophosphate does not serve as a substrate.
Interaction P17169:glmS; NbExp=1; IntAct=EBI-562202, EBI-551022;
Pathway Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1- deoxy-D-xylulose 5-phosphate: step 3/6.
Sequence Caution Sequence=BAA01106.1; Type=Frameshift; Positions=50; Evidence={ECO:0000305};
Similarity Belongs to the GHMP kinase family. IspE subfamily. {ECO:0000305}.
Subunit Homodimer. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007569 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16129171 RefSeq NP_415726 283 4-diphosphocytidyl-2-C-methylerythritol kinase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007569 proteins

Reference Database Accession Length Protein Name
GI:16129171 GenBank KHH93612.1 283 kinase [Escherichia coli]
GI:16129171 GenBank KHH95882.1 283 kinase [Escherichia coli]
GI:16129171 GenBank KHI12146.1 283 kinase [Escherichia coli]
GI:16129171 GenBank KHI18397.1 283 kinase [Escherichia coli]
GI:16129171 GenBank KHI55986.1 283 kinase [Escherichia coli]
GI:16129171 gnl IGS 283 4-diphosphocytidyl-2-C-methylerythritol kinase [Escherichia coli ER2796]

Related Sequences to LMP007569 proteins

Reference Database Accession Length Protein Name
GI:16129171 GenBank EHN97850.1 283 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase [Escherichia coli E101]
GI:16129171 GenBank EIQ11173.1 283 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [Shigella flexneri 2850-71]
GI:16129171 GenBank EIQ13118.1 283 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [Shigella flexneri CCH060]
GI:16129171 GenBank EIQ29589.1 283 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase [Shigella flexneri K-404]
GI:16129171 RefSeq NP_836903.1 283 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase [Shigella flexneri 2a str. 2457T]
GI:16129171 RefSeq WP_001260355.1 283 kinase [Escherichia coli]