Gene/Proteome Database (LMPD)

LMPD ID
LMP007590
Gene ID
949048
Species
Escherichia coli K-12 (E. coli)
Gene Name
3-deoxy-D-manno-octulosonic-acid transferase (KDO transferase)
Gene Symbol
waaA
Synonyms
ECK3623; JW3608; kdtA
Summary
WaaA adds 2 Kdo residues to lipid IVA. waaA is an essential gene. [More information is available at EcoGene: EG10520]. The lipopolysaccharide of Escherichia coli K-12 consists of two major components: the hydrophobic lipid A moiety inserted into the outer membrane and the phosphorylated core oligosaccharide . [More information is available at EcoCyc: EG10520].
Orthologs
View orthologs and multiple alignments for waaA

Proteins

3-deoxy-D-manno-octulosonic-acid transferase (KDO transferase) [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_418090
Protein GI 16131504
UniProt ID P0AC75
Length 425
RefSeq Status REVIEWED
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRHPLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQHVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSAAGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRSFYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH

Gene Information

Entrez Gene ID
949048
Gene Name
3-deoxy-D-manno-octulosonic-acid transferase (KDO transferase)
Gene Symbol
waaA
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:EcoliWiki C membrane
GO:0005886 IDA:EcoCyc C plasma membrane
GO:0016740 IDA:EcoCyc F transferase activity
GO:0036104 IEA:UniProtKB-UniPathway P Kdo2-lipid A biosynthetic process
GO:0009245 IMP:EcoliWiki P lipid A biosynthetic process
GO:0009244 IEA:UniProtKB-UniPathway P lipopolysaccharide core region biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco00540 Lipopolysaccharide biosynthesis
ko00540 Lipopolysaccharide biosynthesis
M00060 Lipopolysaccharide biosynthesis, KDO2-lipid A
eco_M00060 Lipopolysaccharide biosynthesis, KDO2-lipid A
eco01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
KDOSYN-PWY KDO transfer to lipid IVA I
KDOSYN-PWY KDO transfer to lipid IVA I
KDOSYN-PWY KDO transfer to lipid IVA I
KDO-NAGLIPASYN-PWY superpathway of (KDO)2-lipid A biosynthesis
KDO-NAGLIPASYN-PWY superpathway of (KDO)2-lipid A biosynthesis
LPSSYN-PWY superpathway of lipopolysaccharide biosynthesis
LPSSYN-PWY superpathway of lipopolysaccharide biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR007507 3-deoxy-D-manno-octulosonic-acid transferase, N-terminal
IPR001296 Glycosyl transferase, family 1

UniProt Annotations

Entry Information

Gene Name
3-deoxy-D-manno-octulosonic-acid transferase (KDO transferase)
Protein Entry
KDTA_ECOLI
UniProt ID
P0AC75
Species
E. coli

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=52 uM for lipid IV(A) (at pH 8) {ECO:0000269|PubMed:1577828}; KM=88 uM for CMP-Kdo (at pH 8) {ECO:0000269|PubMed:1577828}; Vmax=18 umol/min/mg enzyme (at pH 8) {ECO:0000269|PubMed:1577828}; pH dependence: Optimum pH is 7. {ECO:0000269|PubMed:1577828};
Catalytic Activity Alpha-Kdo-(2->6)-lipid IV(A) + CMP-alpha-Kdo = alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.
Catalytic Activity Lipid IV(A) + CMP-alpha-Kdo = alpha-Kdo- (2->6)-lipid IV(A) + CMP.
Disruption Phenotype Cells lacking this gene display growth defects, absence of Kdo transferase activity, and accumulate massive amounts of lipid IV(A). {ECO:0000269|PubMed:7499229}.
Domain The N-terminal half of KdtA is responsible for determining the number of Kdo residues that are transferred to lipid IVA. {ECO:0000269|PubMed:20394418}.
Enzyme Regulation Catalytic activity is inhibited by the antibiotic polymixin B and by Re endotoxin. {ECO:0000269|PubMed:1577828}.
Function Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of two 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide- 1,4'-bisphosphate precursor of lipid A. {ECO:0000269|PubMed:10951204, ECO:0000269|PubMed:1577828}.
Pathway Bacterial outer membrane biogenesis; LPS core biosynthesis. {ECO:0000269|PubMed:1577828}.
Pathway Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 1/4. {ECO:0000269|PubMed:1577828}.
Pathway Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 2/4. {ECO:0000269|PubMed:1577828}.
Ptm Degraded by the protease FtsH; therefore FtsH regulates the addition of the sugar moiety to the LPS and thus the maturation of the LPS precursor.
Similarity Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 30 subfamily. {ECO:0000305}.
Subcellular Location Cell inner membrane {ECO:0000305|PubMed:1577828}; Single-pass membrane protein {ECO:0000305|PubMed:1577828}; Cytoplasmic side {ECO:0000305|PubMed:1577828}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007590 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16131504 RefSeq NP_418090 425 3-deoxy-D-manno-octulosonic-acid transferase (KDO transferase) [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007590 proteins

Reference Database Accession Length Protein Name
GI:16131504 GenBank KHJ07030.1 425 3-deoxy-D-manno-octulosonic acid transferase [Escherichia coli]
GI:16131504 GenBank KHJ11005.1 425 3-deoxy-D-manno-octulosonic acid transferase [Escherichia coli]
GI:16131504 GenBank KHJ20353.1 425 3-deoxy-D-manno-octulosonic acid transferase [Escherichia coli]
GI:16131504 GenBank KHJ20711.1 425 3-deoxy-D-manno-octulosonic acid transferase [Escherichia coli]
GI:16131504 GenBank KHJ26858.1 425 3-deoxy-D-manno-octulosonic acid transferase [Escherichia coli]
GI:16131504 gnl IGS 425 3-deoxy-D-manno-octulosonic-acid transferase (KDO transferase) [Escherichia coli ER2796]

Related Sequences to LMP007590 proteins

Reference Database Accession Length Protein Name
GI:16131504 GenBank EFJ73656.1 425 3-deoxy-D-manno-octulosonic-acid transferase [Escherichia coli MS 198-1]
GI:16131504 GenBank EFK27522.1 425 3-deoxy-D-manno-octulosonic-acid transferase [Escherichia coli MS 187-1]
GI:16131504 GenBank ESL32645.1 425 3-deoxy-D-manno-octulosonic-acid transferase [Escherichia coli BIDMC 37]
GI:16131504 GenBank KEJ21622.1 425 glycosyl transferases group 1 family protein [Escherichia coli 2-316-03_S1_C1]
GI:16131504 GenBank KHH46655.1 425 3-deoxy-D-manno-octulosonic acid transferase [Escherichia coli]
GI:16131504 RefSeq WP_023282111.1 425 MULTISPECIES: 3-deoxy-D-manno-octulosonic-acid transferase [Enterobacteriaceae]