Gene/Proteome Database (LMPD)
LMPD ID
LMP009687
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Gene Symbol
Synonyms
3-HYDROXY-3-METHYLGLUTARYL COA REDUCTASE; 3-HYDROXY-3-METHYLGLUTARYL COA REDUCTASE 1; F15M4.1; HMG-COA REDUCTASE; HMG1; HMGR1; hydroxy methylglutaryl CoA reductase 1
Alternate Names
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Chromosome
1
EC Number
1.1.1.34
Summary
Encodes a 3-hydroxy-3-methylglutaryl coenzyme A reductase, which is involved in melavonate biosynthesis and performs the first committed step in isoprenoid biosynthesis. Expression is activated in dark in leaf tissue but not controlled by light in the root (confine
Orthologs
Proteins
| 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 | |
|---|---|
| Refseq ID | NP_177775 |
| Protein GI | 79382641 |
| UniProt ID | P14891 |
| mRNA ID | NM_106299 |
| Length | 642 |
| RefSeq Status | REVIEWED |
| MKKKQAGPQQTCEFVSYKTLLISPSHLSRHLTTSLLSPLSPPWRDYSFPPMDLRRRPPKPPVTNNNNSNGSFRSYQPRTSDDDHRRRATTIAPPPKASDALPLPLYLTNAVFFTLFFSVAYYLLHRWRDKIRYNTPLHVVTITELGAIIALIASFIYLLGFFGIDFVQSFISRASGDAWDLADTIDDDDHRLVTCSPPTPIVSVAKLPNPEPIVTESLPEEDEEIVKSVIDGVIPSYSLESRLGDCKRAASIRREALQRVTGRSIEGLPLDGFDYESILGQCCEMPVGYIQIPVGIAGPLLLDGYEYSVPMATTEGCLVASTNRGCKAMFISGGATSTVLKDGMTRAPVVRFASARRASELKFFLENPENFDTLAVVFNRSSRFARLQSVKCTIAGKNAYVRFCCSTGDAMGMNMVSKGVQNVLEYLTDDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAVIRGEIVNKVLKTSVAALVELNMLKNLAGSAVAGSLGGFNAHASNIVSAVFIATGQDPAQNVESSQCITMMEAINDGKDIHISVTMPSIEVGTVGGGTQLASQSACLNLLGVKGASTESPGMNARRLATIVAGAVLAGELSLMSAIAAGQLVRSHMKYNRSSRDISGATTTTTTTT | |
Gene Information
Entrez Gene ID
Gene Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IDA:TAIR | C | endoplasmic reticulum |
| GO:0005789 | IDA:TAIR | C | endoplasmic reticulum membrane |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0042282 | IDA:TAIR | F | hydroxymethylglutaryl-CoA reductase activity |
| GO:0004420 | IEA:UniProtKB-EC | F | hydroxymethylglutaryl-CoA reductase (NADPH) activity |
| GO:0050661 | IEA:InterPro | F | NADP binding |
| GO:0015936 | IEA:InterPro | P | coenzyme A metabolic process |
| GO:0019287 | IDA:TAIR | P | isopentenyl diphosphate biosynthetic process, mevalonate pathway |
| GO:0008299 | IEA:UniProtKB-KW | P | isoprenoid biosynthetic process |
| GO:0055114 | IDA:GOC | P | oxidation-reduction process |
| GO:0060964 | IMP:TAIR | P | regulation of gene silencing by miRNA |
| GO:0016126 | IMP:TAIR | P | sterol biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath_M00095 | C5 isoprenoid biosynthesis, mevalonate pathway |
| ath00900 | Terpenoid backbone biosynthesis |
REACTOME Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR002202 | Hydroxymethylglutaryl-CoA reductase, class I/II |
| IPR023074 | Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic domain |
| IPR023076 | Hydroxymethylglutaryl-CoA reductase, class I/II, conserved site |
| IPR009023 | Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain |
| IPR009029 | Hydroxymethylglutaryl-CoA reductase, class I/II, substrate-binding domain |
| IPR004554 | Hydroxymethylglutaryl-CoA reductase, eukaryotic/arcaheal type |
| IPR023282 | Hydroxymethylglutaryl-CoA reductase, N-terminal |
UniProt Annotations
Entry Information
Gene Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Protein Entry
HMDH1_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative initiation; Named isoforms=2; Name=Short; Synonyms=HMGR1S; IsoId=P14891-1; Sequence=Displayed; Name=Long; Synonyms=HMGR1L; IsoId=P14891-2; Sequence=VSP_041282; |
| Catalytic Activity | (R)-mevalonate + CoA + 2 NADP(+) = (S)-3- hydroxy-3-methylglutaryl-CoA + 2 NADPH. {ECO:0000255|PROSITE- ProRule:PRU10003}. |
| Disruption Phenotype | Dwarfing, early senescence, and sterility. 65% lower levels in triterpenoids content and 50% lower levels in sterol content. Hmg1 and hmg2 double mutants are lethal during male gametophyte development. {ECO:0000269|PubMed:14871314, ECO:0000269|PubMed:17917299, ECO:0000269|PubMed:19363204}. |
| Domain | The N-terminal domain (1-178) of the short isoform is necessary and sufficient for directing the protein to the endoplasmic reticulum and to spherical structures. |
| Enzyme Regulation | Regulated at the post-translational level in response to alterations of sphingolipid and sterol biosynthetic pathways. Negatively regulated by a PP2A-dependent dephosphorylation occurring at a site different than Ser-577. Completely inhibited by mevinolin (IC(50) = 12.5 nM). {ECO:0000269|PubMed:19041104, ECO:0000269|PubMed:21478440, ECO:0000269|PubMed:23404890, ECO:0000269|PubMed:7588795}. |
| Function | Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants. {ECO:0000269|PubMed:14871314, ECO:0000269|PubMed:17917299, ECO:0000269|PubMed:19041104, ECO:0000269|PubMed:19363204}. |
| Induction | Down-regulated by light in immature leaves, but not in roots. Not regulated by myriocin, squalestatin or terbinafine. {ECO:0000269|PubMed:19041104, ECO:0000269|PubMed:8742338, ECO:0000269|PubMed:9107038}. |
| Pathway | Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3. |
| Ptm | Reversibly inactivated by phosphorylation at Ser-577 by spinach or Brassica oleracea HMGR kinases in a cell-free system. Probably also phosphorylated at additional sites. {ECO:0000269|PubMed:10318703, ECO:0000269|PubMed:7588795}. |
| Similarity | Belongs to the HMG-CoA reductase family. {ECO:0000305}. |
| Subcellular Location | Isoform Long: Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| Subcellular Location | Isoform Short: Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=also observed within spherical structures located along the endoplasmic reticulum strands. |
| Subunit | Interacts (via N-terminus) with B''ALPHA and B''BETA. {ECO:0000269|PubMed:21478440}. |
| Tissue Specificity | Found in all tissues. Isoform Short is expressed at low levels specifically in flowers. Expressed in both the tapetum and microspores. {ECO:0000269|PubMed:19363204, ECO:0000269|PubMed:7496400, ECO:0000269|PubMed:8302869}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP009687 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 79382641 | RefSeq | NP_177775 | 642 | 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 |
Identical Sequences to LMP009687 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:79382641 | GenBank | AAR83122.1 | 642 | 3-hydroxy-3-methylglutaryl coenzyme A reductase isoform 1L [Arabidopsis thaliana] |
| GI:79382641 | GenBank | AEE35849.1 | 642 | hydroxy methylglutaryl CoA reductase 1 [Arabidopsis thaliana] |
Related Sequences to LMP009687 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:79382641 | EMBL | CAI70001.1 | 592 | unnamed protein product [Arabidopsis thaliana] |
| GI:79382641 | GenBank | AAA76821.1 | 592 | 3-hydroxy-3-methylglutaryl CoA reductase [Arabidopsis thaliana] |
| GI:79382641 | GenBank | AAA32814.1 | 592 | hydroxymethylglutaryl CoA reductase [Arabidopsis thaliana] |
| GI:79382641 | GenBank | EFH63901.1 | 635 | 3-hydroxy-3-methylglutaryl CoA reductase [Arabidopsis lyrata subsp. lyrata] |
| GI:79382641 | RefSeq | XP_002887642.1 | 635 | 3-hydroxy-3-methylglutaryl CoA reductase [Arabidopsis lyrata subsp. lyrata] |
| GI:79382641 | SwissProt | P14891.1 | 592 | RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase 1; Short=AtHMGR1; Short=HMG-CoA reductase 1 [Arabidopsis thaliana] |