Gene/Proteome Database (LMPD)

LMPD ID
LMP009687
Gene ID
843982
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Gene Symbol
HMG1
Synonyms
3-HYDROXY-3-METHYLGLUTARYL COA REDUCTASE; 3-HYDROXY-3-METHYLGLUTARYL COA REDUCTASE 1; F15M4.1; HMG-COA REDUCTASE; HMG1; HMGR1; hydroxy methylglutaryl CoA reductase 1
Alternate Names
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Chromosome
1
EC Number
1.1.1.34
Summary
Encodes a 3-hydroxy-3-methylglutaryl coenzyme A reductase, which is involved in melavonate biosynthesis and performs the first committed step in isoprenoid biosynthesis. Expression is activated in dark in leaf tissue but not controlled by light in the root (confine
Orthologs
View orthologs and multiple alignments for HMG1

Proteins

3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Refseq ID NP_177775
Protein GI 79382641
UniProt ID P14891
mRNA ID NM_106299
Length 642
RefSeq Status REVIEWED
MKKKQAGPQQTCEFVSYKTLLISPSHLSRHLTTSLLSPLSPPWRDYSFPPMDLRRRPPKPPVTNNNNSNGSFRSYQPRTSDDDHRRRATTIAPPPKASDALPLPLYLTNAVFFTLFFSVAYYLLHRWRDKIRYNTPLHVVTITELGAIIALIASFIYLLGFFGIDFVQSFISRASGDAWDLADTIDDDDHRLVTCSPPTPIVSVAKLPNPEPIVTESLPEEDEEIVKSVIDGVIPSYSLESRLGDCKRAASIRREALQRVTGRSIEGLPLDGFDYESILGQCCEMPVGYIQIPVGIAGPLLLDGYEYSVPMATTEGCLVASTNRGCKAMFISGGATSTVLKDGMTRAPVVRFASARRASELKFFLENPENFDTLAVVFNRSSRFARLQSVKCTIAGKNAYVRFCCSTGDAMGMNMVSKGVQNVLEYLTDDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAVIRGEIVNKVLKTSVAALVELNMLKNLAGSAVAGSLGGFNAHASNIVSAVFIATGQDPAQNVESSQCITMMEAINDGKDIHISVTMPSIEVGTVGGGTQLASQSACLNLLGVKGASTESPGMNARRLATIVAGAVLAGELSLMSAIAAGQLVRSHMKYNRSSRDISGATTTTTTTT

Gene Information

Entrez Gene ID
843982
Gene Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Gene Symbol
HMG1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:TAIR C endoplasmic reticulum
GO:0005789 IDA:TAIR C endoplasmic reticulum membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0050661 IEA:InterPro F NADP binding
GO:0004420 IEA:UniProtKB-EC F hydroxymethylglutaryl-CoA reductase (NADPH) activity
GO:0042282 IDA:TAIR F hydroxymethylglutaryl-CoA reductase activity
GO:0015936 IEA:InterPro P coenzyme A metabolic process
GO:0019287 IDA:TAIR P isopentenyl diphosphate biosynthetic process, mevalonate pathway
GO:0008299 IEA:UniProtKB-KW P isoprenoid biosynthetic process
GO:0055114 IDA:GOC P oxidation-reduction process
GO:0060964 IMP:TAIR P regulation of gene silencing by miRNA
GO:0016126 IMP:TAIR P sterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath_M00095 C5 isoprenoid biosynthesis, mevalonate pathway
ath00900 Terpenoid backbone biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
6254329 Activation of gene expression by SREBF (SREBP)
6254036 Cholesterol biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR023282 Hydroxymethylglutaryl-CoA reductase, N-terminal
IPR002202 Hydroxymethylglutaryl-CoA reductase, class I/II
IPR009023 Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain
IPR023074 Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic domain
IPR023076 Hydroxymethylglutaryl-CoA reductase, class I/II, conserved site
IPR009029 Hydroxymethylglutaryl-CoA reductase, class I/II, substrate-binding domain
IPR004554 Hydroxymethylglutaryl-CoA reductase, eukaryotic/arcaheal type

UniProt Annotations

Entry Information

Gene Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Protein Entry
HMDH1_ARATH
UniProt ID
P14891
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative initiation; Named isoforms=2; Name=Short; Synonyms=HMGR1S; IsoId=P14891-1; Sequence=Displayed; Name=Long; Synonyms=HMGR1L; IsoId=P14891-2; Sequence=VSP_041282;
Catalytic Activity (R)-mevalonate + CoA + 2 NADP(+) = (S)-3- hydroxy-3-methylglutaryl-CoA + 2 NADPH. {ECO:0000255|PROSITE- ProRule:PRU10003}.
Disruption Phenotype Dwarfing, early senescence, and sterility. 65% lower levels in triterpenoids content and 50% lower levels in sterol content. Hmg1 and hmg2 double mutants are lethal during male gametophyte development. {ECO:0000269|PubMed:14871314, ECO:0000269|PubMed:17917299, ECO:0000269|PubMed:19363204}.
Domain The N-terminal domain (1-178) of the short isoform is necessary and sufficient for directing the protein to the endoplasmic reticulum and to spherical structures.
Enzyme Regulation Regulated at the post-translational level in response to alterations of sphingolipid and sterol biosynthetic pathways. Negatively regulated by a PP2A-dependent dephosphorylation occurring at a site different than Ser-577. Completely inhibited by mevinolin (IC(50) = 12.5 nM). {ECO:0000269|PubMed:19041104, ECO:0000269|PubMed:21478440, ECO:0000269|PubMed:23404890, ECO:0000269|PubMed:7588795}.
Function Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants. {ECO:0000269|PubMed:14871314, ECO:0000269|PubMed:17917299, ECO:0000269|PubMed:19041104, ECO:0000269|PubMed:19363204}.
Induction Down-regulated by light in immature leaves, but not in roots. Not regulated by myriocin, squalestatin or terbinafine. {ECO:0000269|PubMed:19041104, ECO:0000269|PubMed:8742338, ECO:0000269|PubMed:9107038}.
Pathway Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Ptm Reversibly inactivated by phosphorylation at Ser-577 by spinach or Brassica oleracea HMGR kinases in a cell-free system. Probably also phosphorylated at additional sites. {ECO:0000269|PubMed:10318703, ECO:0000269|PubMed:7588795}.
Similarity Belongs to the HMG-CoA reductase family. {ECO:0000305}.
Subcellular Location Isoform Long: Endoplasmic reticulum membrane; Multi-pass membrane protein.
Subcellular Location Isoform Short: Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=also observed within spherical structures located along the endoplasmic reticulum strands.
Subunit Interacts (via N-terminus) with B''ALPHA and B''BETA. {ECO:0000269|PubMed:21478440}.
Tissue Specificity Found in all tissues. Isoform Short is expressed at low levels specifically in flowers. Expressed in both the tapetum and microspores. {ECO:0000269|PubMed:19363204, ECO:0000269|PubMed:7496400, ECO:0000269|PubMed:8302869}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009687 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
79382641 RefSeq NP_177775 642 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1

Identical Sequences to LMP009687 proteins

Reference Database Accession Length Protein Name
GI:79382641 GenBank AAR83122.1 642 3-hydroxy-3-methylglutaryl coenzyme A reductase isoform 1L [Arabidopsis thaliana]
GI:79382641 GenBank AEE35849.1 642 hydroxy methylglutaryl CoA reductase 1 [Arabidopsis thaliana]

Related Sequences to LMP009687 proteins

Reference Database Accession Length Protein Name
GI:79382641 EMBL CAI70001.1 592 unnamed protein product [Arabidopsis thaliana]
GI:79382641 GenBank AAA76821.1 592 3-hydroxy-3-methylglutaryl CoA reductase [Arabidopsis thaliana]
GI:79382641 GenBank AAA32814.1 592 hydroxymethylglutaryl CoA reductase [Arabidopsis thaliana]
GI:79382641 GenBank EFH63901.1 635 3-hydroxy-3-methylglutaryl CoA reductase [Arabidopsis lyrata subsp. lyrata]
GI:79382641 RefSeq XP_002887642.1 635 3-hydroxy-3-methylglutaryl CoA reductase [Arabidopsis lyrata subsp. lyrata]
GI:79382641 SwissProt P14891.1 592 RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase 1; Short=AtHMGR1; Short=HMG-CoA reductase 1 [Arabidopsis thaliana]