Gene/Proteome Database (LMPD)
LMPD ID
LMP010075
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
cytochrome P450 734A1
Gene Symbol
Synonyms
CYP72B1; CYP734A1; F18A8.8; F18A8_8; PHYB ACTIVATION TAGGED SUPPRESSOR 1
Alternate Names
cytochrome P450 734A1
Chromosome
2
EC Number
1.14.-.-
Summary
Encodes a member of the cytochrome p450 family that serves as a control point between multiple photoreceptor systems and brassinosteroid signal transduction. Involved in brassinolide metabolism. Mediates response to a variety of light signals including hypocotyl elongation and cotyledon expansion.
Orthologs
Proteins
| cytochrome P450 734A1 | |
|---|---|
| Refseq ID | NP_180239 |
| Protein GI | 15225777 |
| UniProt ID | O48786 |
| mRNA ID | NM_128228 |
| Length | 520 |
| RefSeq Status | REVIEWED |
| MEEESSSWFIPKVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSHPMPFSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEFYEKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAKNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLTNHED | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0020037 | IEA:InterPro | F | heme binding |
| GO:0005506 | IEA:InterPro | F | iron ion binding |
| GO:0016705 | IEA:InterPro | F | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
| GO:0008395 | IMP:TAIR | F | steroid hydroxylase activity |
| GO:0010268 | IEP:TAIR | P | brassinosteroid homeostasis |
| GO:0016131 | IDA:TAIR | P | brassinosteroid metabolic process |
| GO:0040008 | IEA:UniProtKB-KW | P | regulation of growth |
| GO:0009741 | IEP:TAIR | P | response to brassinosteroid |
| GO:0009416 | IGI:TAIR | P | response to light stimulus |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath00905 | Brassinosteroid biosynthesis |
REACTOME Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Cofactor | Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250}; |
| Disruption Phenotype | No visible phenotype under normal growth condition, but reduced responsiveness of hypocotyls to light and increased responsiveness to brassinolide. Strong reduction of C-26 hydroxylase activity. {ECO:0000269|PubMed:14605216, ECO:0000269|PubMed:15773851}. |
| Function | Cytochrome P450 involved in brassinosteroids (BRs) inactivation and regulation of BRs homeostasis. Inactivates the BRs castasterone and brassinolide through carbon 26 hydroxylation. Acts in association with CYP72C1 to inactivate BRs and modulate photomorphogenesis. {ECO:0000269|PubMed:10611382, ECO:0000269|PubMed:14605216, ECO:0000269|PubMed:15773851, ECO:0000269|PubMed:20396969, ECO:0000269|PubMed:20669042}. |
| Induction | By brassinolide, auxin and dark treatment. {ECO:0000269|PubMed:14605216, ECO:0000269|PubMed:20396969}. |
| Miscellaneous | Plants overexpressing CYP734A1 exhibit severe dwarfism. |
| Similarity | Belongs to the cytochrome P450 family. {ECO:0000305}. |
| Subcellular Location | Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010075 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 15225777 | RefSeq | NP_180239 | 520 | cytochrome P450 734A1 |
Identical Sequences to LMP010075 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15225777 | EMBL | CBF74385.1 | 520 | unnamed protein product [Arabidopsis thaliana] |
| GI:15225777 | EMBL | CBU96632.1 | 520 | unnamed protein product [Arabidopsis thaliana] |
| GI:15225777 | GenBank | ACW94596.1 | 520 | Sequence 13863 from patent US 7569389 |
| GI:15225777 | GenBank | AEC07878.1 | 520 | cytochrome P450 734A1 [Arabidopsis thaliana] |
| GI:15225777 | GenBank | AEK18804.1 | 520 | Sequence 421 from patent US 7977535 |
| GI:15225777 | GenBank | AGX64060.1 | 520 | Sequence 28224 from patent US 8541208 |
Related Sequences to LMP010075 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15225777 | GenBank | ABH72934.1 | 520 | Sequence 2 from patent US 7034205 |
| GI:15225777 | GenBank | ACW94597.1 | 493 | Sequence 13864 from patent US 7569389 |
| GI:15225777 | GenBank | EFH55260.1 | 521 | hypothetical protein ARALYDRAFT_901457 [Arabidopsis lyrata subsp. lyrata] |
| GI:15225777 | RefSeq | XP_002879001.1 | 521 | hypothetical protein ARALYDRAFT_901457 [Arabidopsis lyrata subsp. lyrata] |
| GI:15225777 | RefSeq | XP_010510871.1 | 517 | PREDICTED: cytochrome P450 734A1 [Camelina sativa] |
| GI:15225777 | RefSeq | XP_010417817.1 | 517 | PREDICTED: cytochrome P450 734A1-like [Camelina sativa] |