Gene/Proteome Database (LMPD)
LMPD ID
LMP010091
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
sulphotransferase 12
Gene Symbol
Synonyms
ARABIDOPSIS THALIANA SULFOTRANSFERASE 1; AtSOT1; AtSOT12; ATST1; RAR047; SOT1; SOT12; ST; ST1; SULFOTRANSFERASE; SULFOTRANSFERASE 1; SULFURTRANSFERASE; sulphotransferase 1; sulphotransferase 12
Alternate Names
sulphotransferase 12
Chromosome
2
EC Number
2.8.2.-
Summary
Encodes a brassinosteroid sulfotransferase. In vitro experiements show that this enzyme has a preference for 24-epibrassinosteroids, particularly 24-epicathasterone, but does not act on castasterone and brassinolide. It is differentially expressed during development, being more abundant in young seedlings and actively growing cell cultures. Expression is induced in response to salicylic acid and methyl jasmonate and bacterial pathogens.
Orthologs
Proteins
| sulphotransferase 12 | |
|---|---|
| Refseq ID | NP_178471 |
| Protein GI | 15227699 |
| UniProt ID | P52839 |
| mRNA ID | NM_126423 |
| Length | 326 |
| RefSeq Status | REVIEWED |
| MSSSSSVPAYLGDEDLTQETRALISSLPKEKGWLVSEIYEFQGLWHTQAILQGILICQKRFEAKDSDIILVTNPKSGTTWLKALVFALLNRHKFPVSSSGNHPLLVTNPHLLVPFLEGVYYESPDFDFSSLPSPRLMNTHISHLSLPESVKSSSCKIVYCCRNPKDMFVSLWHFGKKLAPEETADYPIEKAVEAFCEGKFIGGPFWDHILEYWYASRENPNKVLFVTYEELKKQTEVEMKRIAEFLECGFIEEEEVREIVKLCSFESLSNLEVNKEGKLPNGIETKTFFRKGEIGGWRDTLSESLAEEIDRTIEEKFKGSGLKFSS | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005794 | IDA:TAIR | C | Golgi apparatus |
| GO:0080118 | IDA:TAIR | F | brassinosteroid sulfotransferase activity |
| GO:1990135 | IDA:TAIR | F | flavonoid sulfotransferase activity |
| GO:0008146 | IDA:TAIR | F | sulfotransferase activity |
| GO:0016131 | IDA:TAIR | P | brassinosteroid metabolic process |
| GO:0006952 | IMP:TAIR | P | defense response |
| GO:0009751 | IMP:TAIR | P | response to salicylic acid |
| GO:0009651 | IEP:TAIR | P | response to salt stress |
REACTOME Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=6.9 uM for 24-epicathasterone {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; KM=1.9 uM for 6-deoxo-24-epicathasterone {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; KM=3.0 uM for 17-beta-estradiol {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; KM=1.1 uM for dehydroepiandrosterone {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; KM=13 uM for pregnenolone {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; KM=0.44 mM for salicylic acid {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; KM=3 uM for 3'-phospho-5'-adenylyl sulfate {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; Vmax=57 pmol/sec/mg enzyme with 24-epicathasterone as substrate {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; Vmax=4.4 pmol/sec/mg enzyme with 24-epicathasterone as substrate {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; Vmax=1.6 pmol/sec/mg enzyme with 17-beta-estradiol as substrate {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; Vmax=1.3 pmol/sec/mg enzyme with dehydroepiandrosterone as substrate {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; Vmax=5.9 pmol/sec/mg enzyme with pregnenolone as substrate {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; Vmax=2.67 pmol/sec/mg enzyme with salicylic acid as substrate {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}; |
| Disruption Phenotype | Hypersensitivity to NaCl and ABA in seed germination, and to salicylic acid (SA) in seedling growth. {ECO:0000269|PubMed:20374532}. |
| Function | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the stereospecific sulfate conjugation of 24-epibrassinosteroids. Preferred substrates are 24-epicathasterone and 6-deoxo-24-epicathasterone. Low activity with 22-deoxy-24-epiteasterone. No activity with 24- epimers catasterone and brassinolide. Sulfonates salicylic acid. May be involved in detoxification. Enhances plant response to pathogen infection and contributes to long distance signaling in systemic acquired resistance (SAR). {ECO:0000269|PubMed:17039368, ECO:0000269|PubMed:20374532}. |
| Induction | Up-regulated by pathogens, methyljasmonate, salicylic acid, salt, osmotic stress, cold, auxin, cytokinin and abscisic acid treatments. Not induced by desiccation and ethylene treatment. {ECO:0000269|PubMed:20374532, ECO:0000269|PubMed:8639757, ECO:0000269|PubMed:9212075}. |
| Sequence Caution | Sequence=CAA80546.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAA86850.1; Type=Frameshift; Positions=300, 313, 317; Evidence={ECO:0000305}; |
| Similarity | Belongs to the sulfotransferase 1 family. {ECO:0000305}. |
| Subcellular Location | Cytoplasm {ECO:0000250}. |
| Subunit | Dimer. {ECO:0000305|PubMed:19173308}. |
| Tissue Specificity | Expressed in the aerial parts of seedlings, in roots, leaves and flowers. Not detected in stems and siliques. {ECO:0000269|PubMed:20374532, ECO:0000269|PubMed:8639757, ECO:0000269|PubMed:9212075}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010091 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 15227699 | RefSeq | NP_178471 | 326 | sulphotransferase 12 |
Identical Sequences to LMP010091 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15227699 | GenBank | ACW88175.1 | 326 | Sequence 5146 from patent US 7569389 |
| GI:15227699 | GenBank | AEC05746.1 | 326 | sulphotransferase 12 [Arabidopsis thaliana] |
| GI:15227699 | GenBank | AGA48821.1 | 326 | Sequence 288 from patent US 8324454 |
| GI:15227699 | PDB | 1Q44 | 326 | Chain A, Crystal Structure Of An Arabidopsis Thaliana Putative Steroid Sulfotransferase |
| GI:15227699 | PDB | 2Q3M | 326 | Chain A, Ensemble Refinement Of The Protein Crystal Structure Of An Arabidopsis Thaliana Putative Steroid Sulphotransferase |
| GI:15227699 | SwissProt | P52839.2 | 326 | RecName: Full=Cytosolic sulfotransferase 12; Short=AtSOT12; AltName: Full=Sulfotransferase 1; Short=AtST1 [Arabidopsis thaliana] |
Related Sequences to LMP010091 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15227699 | EMBL | CAA86850.1 | 302 | Flavonol sulfotransferase [Arabidopsis thaliana] |
| GI:15227699 | GenBank | AAX01883.1 | 302 | Sequence 19 from patent US 6841718 |
| GI:15227699 | GenBank | EFH53155.1 | 325 | hypothetical protein ARALYDRAFT_484285 [Arabidopsis lyrata subsp. lyrata] |
| GI:15227699 | RefSeq | XP_002876896.1 | 325 | hypothetical protein ARALYDRAFT_484285 [Arabidopsis lyrata subsp. lyrata] |
| GI:15227699 | RefSeq | XP_010502398.1 | 325 | PREDICTED: cytosolic sulfotransferase 12-like [Camelina sativa] |
| GI:15227699 | RefSeq | XP_010518598.1 | 325 | PREDICTED: cytosolic sulfotransferase 12 [Camelina sativa] |