Gene/Proteome Database (LMPD)
LMPD ID
LMP010163
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Gene Symbol
Synonyms
F17F16.20
Alternate Names
Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Chromosome
1
EC Number
2.4.1.259
Summary
curculin-like (mannose-binding) lectin family protein, very low similarity to Ser Thr protein kinase GI:2598067 from (Zea mays); contains Pfam lectin (probable mannose binding) domain PF01453 but not the protein kinase domain of the Z. mays protein
Orthologs
Proteins
| Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase | |
|---|---|
| Refseq ID | NP_173134 |
| Protein GI | 145335796 |
| UniProt ID | Q9FZ49 |
| mRNA ID | NM_101551 |
| Length | 570 |
| RefSeq Status | REVIEWED |
| MDLTTTRQRRPLISDSSSSSSTKSYSKTDKPGRSNGGDAEDGGLRWFLPFIALCYLRYMSATSNIIHDCDEVFNYWEPLHYILYKSGFQTWEYSSNFALRSYLYILFHELAGRPASWWFGDDKVRVFYAVRLFLGLVSAVSDTVLVVALSRKYGKRIATYAVAMLCLTSGCFFASTSFLPSSFSMYAISLSSGLLLFEKYAMAVAVSVVGVILGWPFSILAFLPVVIYSLVKRFKQAFIAGAVTTIFLLGVSLLVDYYYYKRWTSSVLNLLIYNVLGGGESHLYGTEGALFYIRNGFNNFNFCFILAMLFVAIYPVIRRKYDRALLVVISPMYIWLAFMSLQPHKEERFLYPIYPLICVSASAVIENIPELFREKYSSRESLLVTITKYMRPVILGCILCASHSRTFALINGYSAPLEVYKLLEHHDDAGPGSVLCVGSEWHRYPSSFFVPHYISEVRWIDDGFRGLLPFPFNNTLGGTSASPPYFNNKNQASEEQYLKNIETCTFLIELQLSRPYQYRGSDLSTWEAIAVLPYLDRELSPAKYRSFFIPHMWQEKNVFGKYVALRRVPK | |
Gene Information
Entrez Gene ID
Gene Name
Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IDA:TAIR | C | endoplasmic reticulum |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0004377 | NAS:UniProtKB | F | GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity |
| GO:0000026 | IGI:TAIR | F | alpha-1,2-mannosyltransferase activity |
| GO:0052926 | IEA:UniProtKB-EC | F | dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity |
| GO:0052918 | IEA:UniProtKB-EC | F | dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity |
| GO:0016301 | IEA:UniProtKB-KW | F | kinase activity |
| GO:0030433 | IGI:TAIR | P | ER-associated ubiquitin-dependent protein catabolic process |
| GO:0097502 | IGI:GOC | P | mannosylation |
| GO:0006490 | NAS:UniProtKB | P | oligosaccharide-lipid intermediate biosynthetic process |
| GO:0006486 | IEA:UniProtKB-UniPathway | P | protein glycosylation |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath01100 | Metabolic pathways |
| ath00510 | N-Glycan biosynthesis |
| ko00510 | N-Glycan biosynthesis |
| ath_M00055 | N-glycan precursor biosynthesis |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR005599 | GPI mannosyltransferase |
UniProt Annotations
Entry Information
Gene Name
Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Protein Entry
ALG9_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Dolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha- (1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc- diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man- alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)- D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. {ECO:0000269|PubMed:20023196}. |
| Catalytic Activity | Dolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)- D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha- (1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)- D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D- GlcNAc-diphosphodolichol + dolichyl phosphate. {ECO:0000269|PubMed:20023196}. |
| Function | Required for N-linked oligosaccharide assembly. Adds the seventh and the ninth mannose residues in an alpha-1,2 linkage onto the dolichol-PP-oligosaccharide precursors dolichol-PP- Man(6)GlcNAc(2) and dolichol-PP-Man(8)GlcNAc(2). {ECO:0000269|PubMed:20023196}. |
| Miscellaneous | In the absence of ALG9 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant. |
| Pathway | Protein modification; protein glycosylation. |
| Similarity | Belongs to the glycosyltransferase 22 family. {ECO:0000305}. |
| Subcellular Location | Endoplasmic reticulum membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010163 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 145335796 | RefSeq | NP_173134 | 570 | Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase |
Identical Sequences to LMP010163 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:145335796 | GenBank | AAF99843.1 | 570 | Hypothetical protein [Arabidopsis thaliana] |
| GI:145335796 | GenBank | AAM98202.1 | 570 | Ser/Thr protein kinase, putative [Arabidopsis thaliana] |
| GI:145335796 | GenBank | AAQ56838.1 | 570 | At1g16900 [Arabidopsis thaliana] |
| GI:145335796 | GenBank | AEE29518.1 | 570 | Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase [Arabidopsis thaliana] |
| GI:145335796 | GenBank | AHL38943.1 | 570 | glycosyltransferase, partial [Arabidopsis thaliana] |
| GI:145335796 | SwissProt | Q9FZ49.1 | 570 | RecName: Full=Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase; AltName: Full=Alpha-1,2-mannosyltransferase ALG9; AltName: Full=Asparagine-linked glycosylation protein 9; AltName: Full=Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase [Arabidopsis thaliana] |
Related Sequences to LMP010163 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:145335796 | GenBank | EFH69186.1 | 570 | sugar binding protein [Arabidopsis lyrata subsp. lyrata] |
| GI:145335796 | GenBank | EOA39935.1 | 603 | hypothetical protein CARUB_v10008626mg, partial [Capsella rubella] |
| GI:145335796 | RefSeq | XP_002892927.1 | 570 | sugar binding protein [Arabidopsis lyrata subsp. lyrata] |
| GI:145335796 | RefSeq | XP_010498021.1 | 571 | PREDICTED: dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase-like [Camelina sativa] |
| GI:145335796 | RefSeq | XP_010459244.1 | 570 | PREDICTED: dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase-like [Camelina sativa] |
| GI:145335796 | RefSeq | XP_010476825.1 | 570 | PREDICTED: dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase isoform X1 [Camelina sativa] |