Gene/Proteome Database (LMPD)

LMPD ID
LMP010164
Gene ID
5007658
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Gene Symbol
ALG12
Synonyms
EBS4; EMS-MUTAGENIZED BRI1(BRASSINOSTEROID INSENSITIVE 1) SUPPRESSOR 4; homolog of asparagine-linked glycosylation 12
Alternate Names
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Chromosome
1
EC Number
2.4.1.260

Proteins

Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Refseq ID NP_001077447
Protein GI 186478048
UniProt ID A8MR93
mRNA ID NM_001083978
Length 332
RefSeq Status REVIEWED
MHDILYHRHHLDSYDHLEFPGVVPRTFIGAFIVSVFASPVVSIISCLGFPKVYSLVAARLVLGCIILSTLRFFRIQIKKKFGNQVETFFVLFTSLQFHFLFYCTRPLPNILALGLVNLAYGNWLKGNFYPALSFLIFATVIFRCDTMLLLGPIGLELLLTKSISFWKALKYCVGTALLAVGLTIFVDSIMWKKFVWPEFEVFWFNSILNRSSDWGRLHQIGHPANVAGEEWVHIDTFGAMNGISRFCEDDFPWRYSKEEEIVVEELRNRNFTYLVNEHSSVDGYKCLFYEEGFERLELRRGFPPIVLVKKAKVYLHREMKKEDPFHKKWPGC
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Refseq ID NP_001117214
Protein GI 186478051
UniProt ID A8MR93
mRNA ID NM_001123742
Length 453
RefSeq Status REVIEWED
MHDILYHRHHLDSYDHLEFPGVVPRTFIGAFIVSVFASPVVSIISCLGFPKVYSLVAARLVLGCIILSTLRFFRIQIKKKFGNQVETFFVLFTSLQFHFLFYCTRPLPNILALGLVNLAYGNWLKGNFYPALSFLIFATVIFRCDTMLLLGPIGLELLLTKSISFWKALKYCVGTALLAVGLTIFVDSIMWKKFVWPEFEVFWFNSILNRSSDWGTHSIHWYFTSALPRSLLVAYPLSLLGTLVDRRVPFFIVPVLSFVILYSKLPHKELRFIISSVPMFNLSAAVAASRIYNNRKKTIWKLVNMVMLAFFAISAGCTVVTFMASYYNYPSGYALKRLHQIGHPANVAGEEWVHIDTFGAMNGISRFCEDDFPWRYSKEEEIVVEELRNRNFTYLVNEHSSVDGYKCLFYEEGFERLELRRGFPPIVLVKKAKVYLHREMKKEDPFHKKWPGC
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Refseq ID NP_001077448
Protein GI 145323718
UniProt ID A8MR93
mRNA ID NM_001083979
Length 497
RefSeq Status REVIEWED
MPTDSKMAKFLQSYGYDLILGSVAAIYVVMAPYTKVEESFNVQSMHDILYHRHHLDSYDHLEFPGVVPRTFIGAFIVSVFASPVVSIISCLGFPKVYSLVAARLVLGCIILSTLRFFRIQIKKKFGNQVETFFVLFTSLQFHFLFYCTRPLPNILALGLVNLAYGNWLKGNFYPALSFLIFATVIFRCDTMLLLGPIGLELLLTKSISFWKALKYCVGTALLAVGLTIFVDSIMWKKFVWPEFEVFWFNSILNRSSDWGTHSIHWYFTSALPRSLLVAYPLSLLGTLVDRRVPFFIVPVLSFVILYSKLPHKELRFIISSVPMFNLSAAVAASRIYNNRKKTIWKLVNMVMLAFFAISAGCTVVTFMASYYNYPSGYALKRLHQIGHPANVAGEEWVHIDTFGAMNGISRFCEDDFPWRYSKEEEIVVEELRNRNFTYLVNEHSSVDGYKCLFYEEGFERLELRRGFPPIVLVKKAKVYLHREMKKEDPFHKKWPGC
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Refseq ID NP_001077446
Protein GI 145323714
UniProt ID A8MR93
mRNA ID NM_001083977
Length 493
RefSeq Status REVIEWED
MPTDSKMAKFLQSYGYDLILGSVAAIYVVMAPYTKVEESFNVQSMHDILYHRHHLDSYDHLEFPGVVPRTFIVSVFASPVVSIISCLGFPKVYSLVAARLVLGCIILSTLRFFRIQIKKKFGNQVETFFVLFTSLQFHFLFYCTRPLPNILALGLVNLAYGNWLKGNFYPALSFLIFATVIFRCDTMLLLGPIGLELLLTKSISFWKALKYCVGTALLAVGLTIFVDSIMWKKFVWPEFEVFWFNSILNRSSDWGTHSIHWYFTSALPRSLLVAYPLSLLGTLVDRRVPFFIVPVLSFVILYSKLPHKELRFIISSVPMFNLSAAVAASRIYNNRKKTIWKLVNMVMLAFFAISAGCTVVTFMASYYNYPSGYALKRLHQIGHPANVAGEEWVHIDTFGAMNGISRFCEDDFPWRYSKEEEIVVEELRNRNFTYLVNEHSSVDGYKCLFYEEGFERLELRRGFPPIVLVKKAKVYLHREMKKEDPFHKKWPGC

Gene Information

Entrez Gene ID
5007658
Gene Name
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Gene Symbol
ALG12
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0000009 IGI:TAIR F alpha-1,6-mannosyltransferase activity
GO:0052917 IEA:UniProtKB-EC F dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity
GO:0030433 IMP:TAIR P ER-associated ubiquitin-dependent protein catabolic process
GO:0097502 IGI:GOC P mannosylation
GO:0006487 IMP:TAIR P protein N-linked glycosylation

KEGG Pathway Links

KEGG Pathway ID Description
ath01100 Metabolic pathways
ath00510 N-Glycan biosynthesis
ko00510 N-Glycan biosynthesis
ath_M00055 N-glycan precursor biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR005599 GPI mannosyltransferase

UniProt Annotations

Entry Information

Gene Name
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Protein Entry
ALG12_ARATH
UniProt ID
A8MR93
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=6; Name=1; IsoId=A8MR93-1; Sequence=Displayed; Name=2; IsoId=A8MR93-2; Sequence=VSP_041716, VSP_041718; Name=3; IsoId=A8MR93-3; Sequence=VSP_041715; Name=4; IsoId=A8MR93-4; Sequence=VSP_041716, VSP_041717; Name=5; IsoId=A8MR93-5; Sequence=VSP_041717; Name=6; IsoId=A8MR93-6; Sequence=VSP_041716;
Catalytic Activity Dolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D- GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha- (1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)- D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha-(1->6))-D-Man- beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. {ECO:0000269|PubMed:20023196}.
Function Required for N-linked oligosaccharide assembly. Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol- PP-oligosaccharide precursor dolichol-PP-Man(7)GlcNAc(2). {ECO:0000269|PubMed:20023196}.
Miscellaneous In the absence of ALG12 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant.
Pathway Protein modification; protein glycosylation.
Similarity Belongs to the glycosyltransferase 22 family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010164 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
145323718 RefSeq NP_001077448 497 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
145323714 RefSeq NP_001077446 493 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
186478051 RefSeq NP_001117214 453 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
186478048 RefSeq NP_001077447 332 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase

Identical Sequences to LMP010164 proteins

Reference Database Accession Length Protein Name
GI:186478048 GenBank ABF59127.1 332 unknown protein [Arabidopsis thaliana]
GI:145323714 GenBank AEE27389.1 493 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:186478048 GenBank AEE27390.1 332 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:145323718 GenBank AEE27391.1 497 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:186478051 GenBank AEE27392.1 453 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:145323718 SwissProt A8MR93.1 497 RecName: Full=Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase; AltName: Full=Alpha-1,6-mannosyltransferase ALG12; AltName: Full=Asparagine-linked glycosylation protein 12; AltName: Full=EMS-mutagenized BRI1 suppressor 4 [Arabidopsis thaliana]

Related Sequences to LMP010164 proteins

Reference Database Accession Length Protein Name
GI:186478051 GenBank ABF59128.1 449 unknown protein [Arabidopsis thaliana]
GI:186478048 GenBank ABF59128.1 449 unknown protein [Arabidopsis thaliana]
GI:145323718 GenBank EFH68282.1 491 hypothetical protein ARALYDRAFT_887214 [Arabidopsis lyrata subsp. lyrata]
GI:145323714 GenBank EFH68282.1 491 hypothetical protein ARALYDRAFT_887214 [Arabidopsis lyrata subsp. lyrata]
GI:186478051 GenBank AEE27389.1 493 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:145323718 GenBank AEE27389.1 493 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:145323714 GenBank AEE27391.1 497 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:186478051 GenBank AEE27391.1 497 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:186478048 GenBank AEE27391.1 497 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:186478048 GenBank AEE27392.1 453 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:145323718 GenBank EOA39086.1 497 hypothetical protein CARUB_v10011809mg [Capsella rubella]
GI:145323718 RefSeq NP_001077446.1 493 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:186478051 RefSeq NP_001077446.1 493 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:145323714 RefSeq NP_001077448.1 497 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:186478051 RefSeq NP_001077448.1 497 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:186478048 RefSeq NP_001077448.1 497 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:186478048 RefSeq NP_001117214.1 453 Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase [Arabidopsis thaliana]
GI:145323718 RefSeq XP_002892023.1 491 hypothetical protein ARALYDRAFT_887214 [Arabidopsis lyrata subsp. lyrata]
GI:145323714 RefSeq XP_002892023.1 491 hypothetical protein ARALYDRAFT_887214 [Arabidopsis lyrata subsp. lyrata]
GI:145323714 RefSeq XP_006306188.1 497 hypothetical protein CARUB_v10011809mg [Capsella rubella]
GI:145323718 RefSeq XP_006306188.1 497 hypothetical protein CARUB_v10011809mg [Capsella rubella]
GI:186478051 SwissProt A8MR93.1 497 RecName: Full=Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase; AltName: Full=Alpha-1,6-mannosyltransferase ALG12; AltName: Full=Asparagine-linked glycosylation protein 12; AltName: Full=EMS-mutagenized BRI1 suppressor 4 [Arabidopsis thaliana]
GI:186478048 SwissProt A8MR93.1 497 RecName: Full=Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase; AltName: Full=Alpha-1,6-mannosyltransferase ALG12; AltName: Full=Asparagine-linked glycosylation protein 12; AltName: Full=EMS-mutagenized BRI1 suppressor 4 [Arabidopsis thaliana]
GI:145323714 SwissProt A8MR93.1 497 RecName: Full=Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase; AltName: Full=Alpha-1,6-mannosyltransferase ALG12; AltName: Full=Asparagine-linked glycosylation protein 12; AltName: Full=EMS-mutagenized BRI1 suppressor 4 [Arabidopsis thaliana]