Gene/Proteome Database (LMPD)

LMPD ID
LMP010554
Gene ID
823650
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
lipoxygenase 2
Gene Symbol
LOX2
Synonyms
ARABIODOPSIS THALIANA LIPOXYGENASE 2; ATLOX2; lipoxygenase 2; LOX2
Alternate Names
lipoxygenase 2
Chromosome
3
EC Number
1.13.11.12
Summary
Chloroplast lipoxygenase required for wound-induced jasmonic acid accumulation in Arabidopsis.Mutants are resistant to Staphylococcus aureus and accumulate salicylic acid upon infection.
Orthologs
View orthologs and multiple alignments for LOX2

Proteins

lipoxygenase 2
Refseq ID NP_566875
Protein GI 18407921
UniProt ID P38418
mRNA ID NM_114383
Length 896
RefSeq Status REVIEWED
MYCRESLSSLQTLNVAKSLSSLFPKQSALINPISAGRRNNLPRPNLRRRCKVTASRANIEQEGNTVKEPIQNIKVKGYITAQEEFLEGITWSRGLDDIADIRGRSLLVELISAKTDQRITVEDYAQRVWAEAPDEKYECEFEMPEDFGPVGAIKIQNQYHRQLFLKGVELKLPGGSITFTCESWVAPKSVDPTKRIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNREEVGEFTKFERIYDYDVYNDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQLPKDAGLLPLLPRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPNINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKRGLAEEDKTAEHGVRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGVVKYELLKPTSEHGVTGMGVPYSISI

Gene Information

Entrez Gene ID
823650
Gene Name
lipoxygenase 2
Gene Symbol
LOX2
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009507 IDA:UniProtKB C chloroplast
GO:0009941 IDA:TAIR C chloroplast envelope
GO:0009570 IDA:TAIR C chloroplast stroma
GO:0009535 IDA:TAIR C chloroplast thylakoid membrane
GO:0005737 IDA:UniProtKB C cytoplasm
GO:0005506 IEA:InterPro F iron ion binding
GO:0016165 IDA:UniProtKB F linoleate 13S-lipoxygenase activity
GO:0009695 IMP:TAIR P jasmonic acid biosynthetic process
GO:0034440 IDA:TAIR P lipid oxidation
GO:0031408 IEA:UniProtKB-UniPathway P oxylipin biosynthetic process
GO:0009617 IEP:UniProtKB P response to bacterium
GO:0009620 IEP:TAIR P response to fungus
GO:0080027 IEP:UniProtKB P response to herbivore
GO:0009753 IEP:TAIR P response to jasmonic acid
GO:0009611 IEP:UniProtKB P response to wounding

KEGG Pathway Links

KEGG Pathway ID Description
ath_M00113 Jasmonic acid biosynthesis
ath00591 Linoleic acid metabolism
ath00592 alpha-Linolenic acid metabolism

REACTOME Pathway Links

REACTOME Pathway ID Description
6254372 Synthesis of 12-eicosatetraenoic acid derivatives

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR013819 Lipoxygenase, C-terminal
IPR020834 Lipoxygenase, conserved site
IPR027433 Lipoxygenase, domain 3
IPR020833 Lipoxygenase, iron binding site
IPR001246 Lipoxygenase, plant
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
lipoxygenase 2
Protein Entry
LOX2_ARATH
UniProt ID
P38418
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity Alpha-linolenate + O(2) = (9Z,11E,13S,15Z)-13- hydroperoxyoctadeca-9,11,15-trienoate.
Catalytic Activity Linoleate + O(2) = (9Z,11E,13S)-13- hydroperoxyoctadeca-9,11-dienoate.
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000255|PROSITE-ProRule:PRU00726}; Note=Binds 1 Fe cation per subunit. Iron is tightly bound. {ECO:0000255|PROSITE-ProRule:PRU00726};
Developmental Stage Expression is sharply reduced in leaves during leaf senescence. {ECO:0000269|PubMed:11891244}.
Function 13S-lipoxygenase that can use linolenic acid as substrates. Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Required for the wound-induced synthesis of jasmonic acid (JA) in leaves. {ECO:0000269|PubMed:18949503, ECO:0000269|PubMed:7567995}.
Induction By methyl jasmonate (MeJA) and wounding, probably through nitric oxide-mediated (NO) induction. Slightly locally induced upon herbivors infestation such as aphids (Myzus persicae and Brevicoryne brassicae), or caterpillar (Spodoptera exigua). Induced by leaf-volatiles generated by herbivors-mediated wounding such as (E)-2-hexenal, (Z)-3-hexenal, (Z)-3-hexenol or allo- ocimene (2,6-dimethyl-2,4,6-octatriene). Increased levels by bacterial pathogens (e.g. P.viridiflava and P.syringae pv. tomato). Repressed by WRKY62. {ECO:0000269|PubMed:11090221, ECO:0000269|PubMed:11161062, ECO:0000269|PubMed:11874572, ECO:0000269|PubMed:14716563, ECO:0000269|PubMed:15879447, ECO:0000269|PubMed:17313166, ECO:0000269|PubMed:17510065, ECO:0000269|PubMed:17545220, ECO:0000269|PubMed:18487634}.
Interaction O04663:EIF(ISO)4E; NbExp=7; IntAct=EBI-1770437, EBI-1770425;
Pathway Lipid metabolism; oxylipin biosynthesis. {ECO:0000255|PROSITE-ProRule:PRU00726}.
Sequence Caution Sequence=CAB72152.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the lipoxygenase family. {ECO:0000305}.
Similarity Contains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}.
Similarity Contains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}.
Subcellular Location Plastid, chloroplast. Cytoplasm. Note=The unprocessed form is cytoplasmic whereas the cleaved form is chloroplastic.
Subunit Interacts with EIF4E2. {ECO:0000269|PubMed:11117257}.
Tissue Specificity In leaves and inflorescences but not abundant in seeds, roots and stems. {ECO:0000269|PubMed:17369372}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010554 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18407921 RefSeq NP_566875 896 lipoxygenase 2

Identical Sequences to LMP010554 proteins

Reference Database Accession Length Protein Name
GI:18407921 GenBank AAA32749.1 896 lipoxygenase [Arabidopsis thaliana]
GI:18407921 GenBank ADT60106.1 896 Sequence 1102 from patent US 7847156
GI:18407921 GenBank AEE77997.1 896 lipoxygenase 2 [Arabidopsis thaliana]
GI:18407921 SwissProt P38418.1 896 RecName: Full=Lipoxygenase 2, chloroplastic; Short=AtLOX2; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP010554 proteins

Reference Database Accession Length Protein Name
GI:18407921 EMBL CAB72152.1 870 lipoxygenase AtLOX2 [Arabidopsis thaliana]
GI:18407921 GenBank AAL32689.1 896 lipoxygenase AtLOX2 [Arabidopsis thaliana]
GI:18407921 GenBank EFH51973.1 896 hypothetical protein ARALYDRAFT_484907 [Arabidopsis lyrata subsp. lyrata]
GI:18407921 RefSeq XP_002875714.1 896 hypothetical protein ARALYDRAFT_484907 [Arabidopsis lyrata subsp. lyrata]
GI:18407921 RefSeq XP_010514856.1 897 PREDICTED: lipoxygenase 2, chloroplastic isoform X1 [Camelina sativa]
GI:18407921 RefSeq XP_010514857.1 897 PREDICTED: lipoxygenase 2, chloroplastic isoform X2 [Camelina sativa]