Gene/Proteome Database (LMPD)

LMPD ID
LMP011082
Gene ID
830095
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
protein brassinosteroid insensitive 1
Gene Symbol
BRI1
Synonyms
ATBRI1; BIN1; BR INSENSITIVE 1; BRASSINOSTEROID INSENSITIVE 1; BRI1; CABBAGE 2; CBB2; DWARF 2; DWF2; F23K16.30; F23K16_30
Alternate Names
protein brassinosteroid insensitive 1
Chromosome
4
EC Number
2.7.10.1
Summary
Encodes a plasma membrane localized leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. BRI1 ligand is brassinolide which binds at the extracellular domain. Binding results in phosphorylation of the kinase domain which activates the BRI1 protein leading to BR responses. Residue T-1049 and either S-1044 or T-1045 were essential for kinase function in vitro and normal BRI1 signaling in planta. Although BAK1 and BRI1 alone localize in the plasma membrane, when BAK1 and BRI1 are coexpressed, the heterodimer BAK1/BRI1 they form is localized in the endosome. BRI1 appears to be involved in the autonomous pathway that regulates the transition to flowering, primarily through its effects on FLC expression levels, as uncovered by double mutant analyses. This most likely occurs as a result of BRI1-dependent effects on histone acetylation, but not histone triMeH3K4 methylation, at the FLC locus.
Orthologs
View orthologs and multiple alignments for BRI1

Proteins

protein brassinosteroid insensitive 1
Refseq ID NP_195650
Protein GI 15235059
UniProt ID O22476
mRNA ID NM_120100
Length 1196
RefSeq Status REVIEWED
MKTFSSFFLSVTTLFFFSFFSLSFQASPSQSLYREIHQLISFKDVLPDKNLLPDWSSNKNPCTFDGVTCRDDKVTSIDLSSKPLNVGFSAVSSSLLSLTGLESLFLSNSHINGSVSGFKCSASLTSLDLSRNSLSGPVTTLTSLGSCSGLKFLNVSSNTLDFPGKVSGGLKLNSLEVLDLSANSISGANVVGWVLSDGCGELKHLAISGNKISGDVDVSRCVNLEFLDVSSNNFSTGIPFLGDCSALQHLDISGNKLSGDFSRAISTCTELKLLNISSNQFVGPIPPLPLKSLQYLSLAENKFTGEIPDFLSGACDTLTGLDLSGNHFYGAVPPFFGSCSLLESLALSSNNFSGELPMDTLLKMRGLKVLDLSFNEFSGELPESLTNLSASLLTLDLSSNNFSGPILPNLCQNPKNTLQELYLQNNGFTGKIPPTLSNCSELVSLHLSFNYLSGTIPSSLGSLSKLRDLKLWLNMLEGEIPQELMYVKTLETLILDFNDLTGEIPSGLSNCTNLNWISLSNNRLTGEIPKWIGRLENLAILKLSNNSFSGNIPAELGDCRSLIWLDLNTNLFNGTIPAAMFKQSGKIAANFIAGKRYVYIKNDGMKKECHGAGNLLEFQGIRSEQLNRLSTRNPCNITSRVYGGHTSPTFDNNGSMMFLDMSYNMLSGYIPKEIGSMPYLFILNLGHNDISGSIPDEVGDLRGLNILDLSSNKLDGRIPQAMSALTMLTEIDLSNNNLSGPIPEMGQFETFPPAKFLNNPGLCGYPLPRCDPSNADGYAHHQRSHGRRPASLAGSVAMGLLFSFVCIFGLILVGREMRKRRRKKEAELEMYAEGHGNSGDRTANNTNWKLTGVKEALSINLAAFEKPLRKLTFADLLQATNGFHNDSLIGSGGFGDVYKAILKDGSAVAIKKLIHVSGQGDREFMAEMETIGKIKHRNLVPLLGYCKVGDERLLVYEFMKYGSLEDVLHDPKKAGVKLNWSTRRKIAIGSARGLAFLHHNCSPHIIHRDMKSSNVLLDENLEARVSDFGMARLMSAMDTHLSVSTLAGTPGYVPPEYYQSFRCSTKGDVYSYGVVLLELLTGKRPTDSPDFGDNNLVGWVKQHAKLRISDVFDPELMKEDPALEIELLQHLKVAVACLDDRAWRRPTMVQVMAMFKEIQAGSGIDSQSTIRSIEDGGFSTIEMVDMSIKEVPEGKL

Gene Information

Entrez Gene ID
830095
Gene Name
protein brassinosteroid insensitive 1
Gene Symbol
BRI1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005768 IDA:TAIR C endosome
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005886 IDA:TAIR C plasma membrane
GO:0043234 IPI:TAIR C protein complex
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0042802 IPI:IntAct F identical protein binding
GO:0046982 IPI:TAIR F protein heterodimerization activity
GO:0042803 IDA:TAIR F protein homodimerization activity
GO:0004672 IDA:TAIR F protein kinase activity
GO:0004674 IDA:TAIR F protein serine/threonine kinase activity
GO:0005496 IDA:TAIR F steroid binding
GO:0004714 IEA:UniProtKB-EC F transmembrane receptor protein tyrosine kinase activity
GO:0048657 IMP:TAIR P anther wall tapetum cell differentiation
GO:0010268 IEP:TAIR P brassinosteroid homeostasis
GO:0009742 IMP:TAIR P brassinosteroid mediated signaling pathway
GO:0009729 IMP:TAIR P detection of brassinosteroid stimulus
GO:0048366 IMP:TAIR P leaf development
GO:0060548 IMP:UniProtKB P negative regulation of cell death
GO:0010584 IMP:TAIR P pollen exine formation
GO:0009911 IGI:TAIR P positive regulation of flower development
GO:1900140 IMP:UniProtKB P regulation of seedling development
GO:0010224 IGI:TAIR P response to UV-B

KEGG Pathway Links

KEGG Pathway ID Description
ath04075 Plant hormone signal transduction
ko04075 Plant hormone signal transduction

Domain Information

InterPro Annotations

Accession Description
IPR013320 Concanavalin A-like lectin/glucanase domain
IPR001611 Leucine-rich repeat
IPR013210 Leucine-rich repeat-containing N-terminal, type 2
IPR000719 Protein kinase domain
IPR017441 Protein kinase, ATP binding site
IPR011009 Protein kinase-like domain
IPR008271 Serine/threonine-protein kinase, active site

UniProt Annotations

Entry Information

Gene Name
protein brassinosteroid insensitive 1
Protein Entry
BRI1_ARATH
UniProt ID
O22476
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. {ECO:0000255|PROSITE- ProRule:PRU10027}.
Catalytic Activity ATP + a protein = ADP + a phosphoprotein.
Developmental Stage Expressed constitutively in either dark- or light-grown seedlings.
Disruption Phenotype Dwarf phenotype and aberrant leaf shape. {ECO:0000269|PubMed:10557222}.
Domain A 70 amino acid island between the 20th and the 21th LRR is essential for the binding of brassinosteroids. {ECO:0000269|PubMed:17520012}.
Domain A guanylyl cyclase domain (1021-1134) having an in vitro activity is included in the C-terminal kinase domain. {ECO:0000269|PubMed:17520012}.
Domain Contains one leucine-zipper motif and two pairs of conservatively spaced Cys (Cys pair 1 and 2) involved in forming heterodimers. {ECO:0000269|PubMed:17520012}.
Domain The JM domain (815-883) is a positive regulator of kinase activity and is required for Tyr phosphorylation. {ECO:0000269|PubMed:17520012}.
Enzyme Regulation Activated by Ser and Thr phosphorylation.
Function Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Regulates, in response to brassinosteroid binding, a signaling cascade involved in plant development, including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Binds brassinolide, and less effectively castasterone, but not 2,3,22,23-O-tetramethylbrassinolide or ecdysone. May be involved in a feedback regulation of brassinosteroid biosynthesis. Phosphorylates BRI1-associated receptor kinase 1 (BAK1), Transthyretin-Like protein (TTL) and SERK1 on 'Ser-299' and 'Thr- 462' in vitro. May have a guanylyl cyclase activity. {ECO:0000269|PubMed:10557222, ECO:0000269|PubMed:10938344, ECO:0000269|PubMed:17138891, ECO:0000269|PubMed:17520012, ECO:0000269|PubMed:18694562, ECO:0000269|PubMed:19124768}.
Interaction Self; NbExp=3; IntAct=EBI-1797828, EBI-1797828; Q944A7:At4g35230; NbExp=4; IntAct=EBI-1797828, EBI-1797846; Q94F62:BAK1; NbExp=5; IntAct=EBI-1797828, EBI-617138; O65280:F6N23.9; NbExp=2; IntAct=EBI-1797828, EBI-1797930; Q94AG2:SERK1; NbExp=5; IntAct=EBI-1797828, EBI-1555537; Q9LVM5:TTL; NbExp=3; IntAct=EBI-1797828, EBI-1803584;
Miscellaneous Binding of brassinosteroid induces intramolecular autophosphorylation of BRI1. Interaction with BAK1 activates both receptor kinases and the full activation of either receptor kinase requires transphosphorylation by their partners. Optimum in vitro phosphorylation of the substrate requires Arg or Lys residues at P-3, P-4, and P+5 (relative to the phosphorylated amino acid at P=0). Homodimerizes in the absence of ligand and binds brassinosteroid in the absence of its coreceptor BAK1.
Miscellaneous The bri1-9 mutation produces a fully active protein with a subtle conformational change that is recognized for reglucosylation by UGGT, resulting in its endoplasmic reticulum retention via Glc(1)Man(9)GlcNAc(2)-calreticulin/calnexin interaction. {ECO:0000305|PubMed:17588517}.
Ptm Autophosphorylated on Tyr-831, Tyr-956 and maybe Tyr-1072. Phosphorylated on at least 12 sites, with a preference for Ser residues. Transphosphorylated on Ser-887 by SERK1 and on Ser-838, Thr-846, Ser-858 and Ser-1166 by BAK1. Phosphorylation on Ser-1166 enhances the kinase activity. {ECO:0000269|PubMed:11027724, ECO:0000269|PubMed:12150928, ECO:0000269|PubMed:12150929, ECO:0000269|PubMed:15894717, ECO:0000269|PubMed:18694562, ECO:0000269|PubMed:19105183, ECO:0000269|PubMed:19124768}.
Ptm Glycosylated. {ECO:0000269|PubMed:17588517}.
Similarity Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
Similarity Contains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}.
Similarity Contains 25 LRR (leucine-rich) repeats. {ECO:0000305}.
Subcellular Location Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein.
Subunit Monomer or homodimer in the plasma membrane. Heterodimer with BAK1 in the endosomes. Interacts with SERK1 and TTL in a kinase-dependent manner. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1. Interacts with CDG1 (PubMed:21855796). {ECO:0000269|PubMed:12150928, ECO:0000269|PubMed:12150929, ECO:0000269|PubMed:15319482, ECO:0000269|PubMed:15548744, ECO:0000269|PubMed:15894717, ECO:0000269|PubMed:16473966, ECO:0000269|PubMed:17905839, ECO:0000269|PubMed:18694562, ECO:0000269|PubMed:21855796}.
Tissue Specificity Expressed ubiquitously. {ECO:0000269|PubMed:10938344, ECO:0000269|PubMed:9298904}.
Web Resource Name=PlantP kinase Classification PPC; URL="http://plantsp.genomics.purdue.edu/family/class.html";

Identical and Related Proteins

Unique RefSeq proteins for LMP011082 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15235059 RefSeq NP_195650 1196 protein brassinosteroid insensitive 1

Identical Sequences to LMP011082 proteins

Reference Database Accession Length Protein Name
GI:15235059 GenBank ABL51228.1 1196 Sequence 6 from patent US 7135625
GI:15235059 GenBank ACN00572.1 1196 Sequence 100 from patent US 7476777
GI:15235059 GenBank ACN59359.1 1196 leucine-rich repeat receptor-like protein kinase, partial [Arabidopsis thaliana]
GI:15235059 GenBank ACX20369.1 1196 Sequence 48874 from patent US 7569389
GI:15235059 GenBank AEE87069.1 1196 protein brassinosteroid insensitive 1 [Arabidopsis thaliana]
GI:15235059 SwissProt O22476.1 1196 RecName: Full=Protein BRASSINOSTEROID INSENSITIVE 1; Short=AtBRI1; AltName: Full=Brassinosteroid LRR receptor kinase; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP011082 proteins

Reference Database Accession Length Protein Name
GI:15235059 GenBank AAE74449.1 1196 Sequence 2 from patent US 6245969
GI:15235059 GenBank AAV24594.1 1196 Sequence 2 from patent US 6765085
GI:15235059 GenBank EFH43106.1 1195 hypothetical protein ARALYDRAFT_912402 [Arabidopsis lyrata subsp. lyrata]
GI:15235059 GenBank EOA15434.1 1196 hypothetical protein CARUB_v10004010mg [Capsella rubella]
GI:15235059 RefSeq XP_002866847.1 1195 hypothetical protein ARALYDRAFT_912402 [Arabidopsis lyrata subsp. lyrata]
GI:15235059 RefSeq XP_006282536.1 1196 hypothetical protein CARUB_v10004010mg [Capsella rubella]