Gene/Proteome Database (LMPD)
LMPD ID
LMP011132
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
protein phosphatase 2C 56
Gene Symbol
Synonyms
ABA INSENSITIVE 1; ABI1; AtABI1; F20B18.190; F20B18_190; PROTEIN PHOSPHATASE 2C ABI1
Alternate Names
protein phosphatase 2C 56
Chromosome
4
EC Number
3.1.3.16
Summary
Involved in abscisic acid (ABA) signal transduction. Negative regulator of ABA promotion of stomatal closure.
Orthologs
Proteins
| protein phosphatase 2C 56 | |
|---|---|
| Refseq ID | NP_194338 |
| Protein GI | 15236110 |
| UniProt ID | P49597 |
| mRNA ID | NM_118741 |
| Length | 434 |
| RefSeq Status | REVIEWED |
| MEEVSPAIAGPFRPFSETQMDFTGIRLGKGYCNNQYSNQDSENGDLMVSLPETSSCSVSGSHGSESRKVLISRINSPNLNMKESAAADIVVVDISAGDEINGSDITSEKKMISRTESRSLFEFKSVPLYGFTSICGRRPEMEDAVSTIPRFLQSSSGSMLDGRFDPQSAAHFFGVYDGHGGSQVANYCRERMHLALAEEIAKEKPMLCDGDTWLEKWKKALFNSFLRVDSEIESVAPETVGSTSVVAVVFPSHIFVANCGDSRAVLCRGKTALPLSVDHKPDREDEAARIEAAGGKVIQWNGARVFGVLAMSRSIGDRYLKPSIIPDPEVTAVKRVKEDDCLILASDGVWDVMTDEEACEMARKRILLWHKKNAVAGDASLLADERRKEGKDPAAMSAAEYLSKLAIQRGSKDNISVVVVDLKPRRKLKSKPLN | |
Gene Information
Entrez Gene ID
Gene Name
protein phosphatase 2C 56
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IEA:UniProtKB-KW | C | cytoplasm |
| GO:0005634 | IDA:TAIR | C | nucleus |
| GO:0005886 | IEA:UniProtKB-KW | C | plasma membrane |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0019901 | IPI:UniProtKB | F | protein kinase binding |
| GO:0004722 | IDA:TAIR | F | protein serine/threonine phosphatase activity |
| GO:0009738 | IEA:UniProtKB-KW | P | abscisic acid-activated signaling pathway |
| GO:0009788 | IGI:UniProtKB | P | negative regulation of abscisic acid-activated signaling pathway |
| GO:0006470 | IEA:InterPro | P | protein dephosphorylation |
| GO:0009787 | IMP:TAIR | P | regulation of abscisic acid-activated signaling pathway |
| GO:0010119 | IMP:TAIR | P | regulation of stomatal movement |
| GO:0009737 | IMP:TAIR | P | response to abscisic acid |
| GO:0009409 | IMP:TAIR | P | response to cold |
| GO:0009408 | IMP:TAIR | P | response to heat |
KEGG Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | pH dependence: Optimum pH is 8. {ECO:0000269|PubMed:9537523}; |
| Catalytic Activity | [a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate. {ECO:0000269|PubMed:10645425, ECO:0000269|PubMed:8898906, ECO:0000269|PubMed:9537523}. |
| Cofactor | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:8898906, ECO:0000269|PubMed:9537523}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:8898906, ECO:0000269|PubMed:9537523}; Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000269|PubMed:8898906, ECO:0000269|PubMed:9537523}; |
| Domain | The 'lock' site stabilizes the complex made of PP2C, ABA and PYR/PYL/RCAR receptor by keeping receptor 'gate' and 'latch' loops in closed positions. |
| Enzyme Regulation | Phosphatase activity repressed by oxidized GPX3 and phosphatidic acid (PA). PA is produced by PLD alpha 1 in response to ABA. Repressed by PYR/PYL/RCAR ABA receptors in an ABA-dependent manner. {ECO:0000269|PubMed:15197253, ECO:0000269|PubMed:16614222, ECO:0000269|PubMed:19407142, ECO:0000269|PubMed:19769575}. |
| Function | Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as the actin reorganization in guard cells in response to ABA. Involved in the resistance to the bacterial pathogen Pseudomonas syringae pv. tomato. Controls negatively fibrillin expression that is involved in mediating ABA-induced photoprotection. May be involved in ABA content regulation. Plays a role in the Pro accumulation in response to reduced water availability (low water potential). Required for the ABA negative regulation of the ethylene-induced hyponastic growth. Involved in acquired thermotolerance of root growth and seedling survival. Activates/represses SRK2E/OST1 in response to ABA-dependent stimuli, especially in stomata closure regulation involving SLAC1. {ECO:0000269|PubMed:10488243, ECO:0000269|PubMed:10521520, ECO:0000269|PubMed:10645425, ECO:0000269|PubMed:10872217, ECO:0000269|PubMed:10950871, ECO:0000269|PubMed:11208021, ECO:0000269|PubMed:11289613, ECO:0000269|PubMed:11587514, ECO:0000269|PubMed:11701885, ECO:0000269|PubMed:11707572, ECO:0000269|PubMed:12047634, ECO:0000269|PubMed:12065416, ECO:0000269|PubMed:12194854, ECO:0000269|PubMed:12228349, ECO:0000269|PubMed:12232124, ECO:0000269|PubMed:12232276, ECO:0000269|PubMed:12432076, ECO:0000269|PubMed:12609042, ECO:0000269|PubMed:12713537, ECO:0000269|PubMed:14576281, ECO:0000269|PubMed:14596925, ECO:0000269|PubMed:15144382, ECO:0000269|PubMed:15197253, ECO:0000269|PubMed:15618419, ECO:0000269|PubMed:15923322, ECO:0000269|PubMed:16339784, ECO:0000269|PubMed:16365038, ECO:0000269|PubMed:16571665, ECO:0000269|PubMed:16614222, ECO:0000269|PubMed:16652949, ECO:0000269|PubMed:16798945, ECO:0000269|PubMed:17158582, ECO:0000269|PubMed:17304219, ECO:0000269|PubMed:18298671, ECO:0000269|PubMed:1834244, ECO:0000269|PubMed:19955405, ECO:0000269|PubMed:7568166, ECO:0000269|PubMed:8492808, ECO:0000269|PubMed:8771791, ECO:0000269|PubMed:8898906, ECO:0000269|PubMed:9090884, ECO:0000269|PubMed:9108297, ECO:0000269|PubMed:9161030, ECO:0000269|PubMed:9165752, ECO:0000269|PubMed:9263461, ECO:0000269|PubMed:9276963, ECO:0000269|PubMed:9351242, ECO:0000269|PubMed:9448270, ECO:0000269|Ref.8}. |
| Induction | Repressed by MYB44. Induced by low temperature, drought, high salt, ABA and ethylene. {ECO:0000269|PubMed:11439132, ECO:0000269|PubMed:14731256, ECO:0000269|PubMed:16339800, ECO:0000269|PubMed:16998070, ECO:0000269|PubMed:17158582, ECO:0000269|PubMed:18162593}. |
| Interaction | O80920:PYL4; NbExp=3; IntAct=EBI-782526, EBI-2349683; Q9FLB1:PYL5; NbExp=3; IntAct=EBI-782526, EBI-2363181; Q84MC7:PYL9; NbExp=4; IntAct=EBI-782526, EBI-2349513; O49686:PYR1; NbExp=7; IntAct=EBI-782526, EBI-2349590; Q39192:SRK2D; NbExp=4; IntAct=EBI-782526, EBI-2363308; Q940H6:SRK2E; NbExp=4; IntAct=EBI-782526, EBI-782514; Q39193:SRK2I; NbExp=3; IntAct=EBI-782526, EBI-2620383; |
| Miscellaneous | Enhanced ABA signaling repressor activity by the proteasomal inhibitor MG132 accompanied by a cytoplasmic localization. |
| Miscellaneous | Plants insensitive to ABA (abi1-1) are more resistant to P.syringae. |
| Similarity | Belongs to the PP2C family. {ECO:0000305}. |
| Similarity | Contains 1 PP2C-like domain. {ECO:0000305}. |
| Subcellular Location | Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein. Note=Associated to the plasma membrane when in complex with PA, subsequently to ABA signaling. |
| Subunit | Interacts with ATHB-6, CIPK15/PKS3, GPX3, SRK2E/OST1, SRK2D, SRK2I, SCAR1, SCAR2, SCAR3 and SCARL. Binds to the PA released by the phospholipase D alpha 1 (PLDALPHA1) in response to ABA during the stomatal closure regulation. Interacts with ABA- bounded PYR1, PYL1, PYL2, PYL3, PYL4, PYL5, PYL6, PYL7, PYL8, PYL9, PYL10, and with free PYL2, PYL3 and PYL4. Binds to RPL12B, CPK21 and CPK23. {ECO:0000269|PubMed:12065416, ECO:0000269|PubMed:12194854, ECO:0000269|PubMed:15197253, ECO:0000269|PubMed:16365038, ECO:0000269|PubMed:16614222, ECO:0000269|PubMed:16998070, ECO:0000269|PubMed:17267444, ECO:0000269|PubMed:19407142, ECO:0000269|PubMed:19407143, ECO:0000269|PubMed:19769575, ECO:0000269|PubMed:19855379, ECO:0000269|PubMed:19874541, ECO:0000269|PubMed:19893533, ECO:0000269|PubMed:19933100, ECO:0000269|PubMed:19955405, ECO:0000269|PubMed:20385816}. |
| Tissue Specificity | Expressed in seeds and seedlings. In roots, confined to lateral root caps and columella cells. {ECO:0000269|PubMed:16339800, ECO:0000269|PubMed:18298671}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP011132 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 15236110 | RefSeq | NP_194338 | 434 | protein phosphatase 2C 56 |
Identical Sequences to LMP011132 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15236110 | EMBL | CBC20172.1 | 434 | unnamed protein product [Arabidopsis thaliana] |
| GI:15236110 | GenBank | ADT60284.1 | 434 | Sequence 1458 from patent US 7847156 |
| GI:15236110 | GenBank | AEE85155.1 | 434 | protein phosphatase 2C 56 [Arabidopsis thaliana] |
| GI:15236110 | GenBank | AFC15991.1 | 434 | Sequence 4 from patent US 8115052 |
| GI:15236110 | GenBank | AFX49417.1 | 434 | Sequence 51250 from patent US 8299318 |
| GI:15236110 | GenBank | AGF15300.1 | 434 | Sequence 14719 from patent US 8362325 |
Related Sequences to LMP011132 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15236110 | EMBL | CAA55484.1 | 434 | ABI1 [Arabidopsis thaliana] |
| GI:15236110 | GenBank | AAK59578.1 | 434 | putative protein phosphatase ABI1 [Arabidopsis thaliana] |
| GI:15236110 | GenBank | ABZ53444.1 | 434 | Sequence 46 from patent US 7317140 |
| GI:15236110 | GenBank | EFH43840.1 | 434 | hypothetical protein ARALYDRAFT_492207 [Arabidopsis lyrata subsp. lyrata] |
| GI:15236110 | GenBank | AEJ76809.1 | 434 | Sequence 46 from patent US 7964769 |
| GI:15236110 | GenBank | AFX04097.1 | 434 | Sequence 46 from patent US 8278506 |