Gene/Proteome Database (LMPD)
Proteins
| protein phosphatase 2C 77 | |
|---|---|
| Refseq ID | NP_200515 |
| Protein GI | 15242022 |
| UniProt ID | O04719 |
| mRNA ID | NM_125087 |
| Length | 423 |
| RefSeq Status | REVIEWED |
| MDEVSPAVAVPFRPFTDPHAGLRGYCNGESRVTLPESSCSGDGAMKDSSFEINTRQDSLTSSSSAMAGVDISAGDEINGSDEFDPRSMNQSEKKVLSRTESRSLFEFKCVPLYGVTSICGRRPEMEDSVSTIPRFLQVSSSSLLDGRVTNGFNPHLSAHFFGVYDGHGGSQVANYCRERMHLALTEEIVKEKPEFCDGDTWQEKWKKALFNSFMRVDSEIETVAHAPETVGSTSVVAVVFPTHIFVANCGDSRAVLCRGKTPLALSVDHKPDRDDEAARIEAAGGKVIRWNGARVFGVLAMSRSIGDRYLKPSVIPDPEVTSVRRVKEDDCLILASDGLWDVMTNEEVCDLARKRILLWHKKNAMAGEALLPAEKRGEGKDPAAMSAAEYLSKMALQKGSKDNISVVVVDLKGIRKFKSKSLN | |
| protein phosphatase 2C 77 | |
|---|---|
| Refseq ID | NP_001119448 |
| Protein GI | 186532520 |
| UniProt ID | O04719 |
| mRNA ID | NM_001125976 |
| Length | 383 |
| RefSeq Status | REVIEWED |
| MDEVSPAVAVPFRPFTDPHAGLRGYCNGVDISAGDEINGSDEFDPRSMNQSEKKVLSRTESRSLFEFKCVPLYGVTSICGRRPEMEDSVSTIPRFLQVSSSSLLDGRVTNGFNPHLSAHFFGVYDGHGGSQVANYCRERMHLALTEEIVKEKPEFCDGDTWQEKWKKALFNSFMRVDSEIETVAHAPETVGSTSVVAVVFPTHIFVANCGDSRAVLCRGKTPLALSVDHKPDRDDEAARIEAAGGKVIRWNGARVFGVLAMSRSIGDRYLKPSVIPDPEVTSVRRVKEDDCLILASDGLWDVMTNEEVCDLARKRILLWHKKNAMAGEALLPAEKRGEGKDPAAMSAAEYLSKMALQKGSKDNISVVVVDLKGIRKFKSKSLN | |
Gene Information
Entrez Gene ID
Gene Name
protein phosphatase 2C 77
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0004722 | ISS:TAIR | F | protein serine/threonine phosphatase activity |
| GO:0009738 | IEA:UniProtKB-KW | P | abscisic acid-activated signaling pathway |
| GO:0009788 | IGI:TAIR | P | negative regulation of abscisic acid-activated signaling pathway |
| GO:0006469 | IDA:TAIR | P | negative regulation of protein kinase activity |
| GO:0010205 | IMP:TAIR | P | photoinhibition |
| GO:0006470 | IEA:InterPro | P | protein dephosphorylation |
| GO:1902456 | IMP:TAIR | P | regulation of stomatal opening |
| GO:0009737 | IMP:TAIR | P | response to abscisic acid |
| GO:0009408 | IMP:TAIR | P | response to heat |
| GO:0006970 | IMP:TAIR | P | response to osmotic stress |
| GO:0009414 | IMP:TAIR | P | response to water deprivation |
KEGG Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=O04719-1; Sequence=Displayed; Name=2; IsoId=O04719-2; Sequence=VSP_034834; Note=Derived from EST data. No experimental confirmation available.; |
| Biophysicochemical Properties | pH dependence: Optimum pH is 8. {ECO:0000269|PubMed:11882947}; |
| Catalytic Activity | [a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate. |
| Cofactor | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:11882947}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:11882947}; Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000269|PubMed:11882947}; |
| Domain | The 'lock' site stabilizes the complex made of PP2C, ABA and PYR/PYL/RCAR receptor by keeping receptor 'gate' and 'latch' loops in closed positions. {ECO:0000250}. |
| Enzyme Regulation | Phosphatase activity repressed by oxidized ATGPX3, free fatty acids (e.g. arachidonic acid (20:4) and Linolenic acid (18:3)) and by H(2)O(2). Repressed by PYR/PYL/RCAR ABA receptors in an ABA-dependent manner. {ECO:0000269|PubMed:11882947, ECO:0000269|PubMed:19407142, ECO:0000269|PubMed:19769575}. |
| Function | Repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), high light stress, response to glucose, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as H(2)O(2) and oxidative burst in response to ABA and dehydration. Controls negatively fibrillin that is involved in mediating ABA- induced photoprotection. May be implicated in ABA content regulation. Involved in acquired thermotolerance of root growth and seedling survival. Required for the Erwinia amylovora harpin- induced (HrpN) drought tolerance. Involved in the hydrotropic response. {ECO:0000269|PubMed:10488243, ECO:0000269|PubMed:10872217, ECO:0000269|PubMed:10950871, ECO:0000269|PubMed:11208021, ECO:0000269|PubMed:11701885, ECO:0000269|PubMed:11707572, ECO:0000269|PubMed:12119381, ECO:0000269|PubMed:12194854, ECO:0000269|PubMed:12232276, ECO:0000269|PubMed:12447533, ECO:0000269|PubMed:12609042, ECO:0000269|PubMed:14576281, ECO:0000269|PubMed:14596925, ECO:0000269|PubMed:15599761, ECO:0000269|PubMed:15923322, ECO:0000269|PubMed:16339784, ECO:0000269|PubMed:16571665, ECO:0000269|PubMed:18278579, ECO:0000269|PubMed:8787023, ECO:0000269|PubMed:9090884, ECO:0000269|PubMed:9108297, ECO:0000269|PubMed:9165752, ECO:0000269|PubMed:9276963}. |
| Induction | Repressed by MYB44 and ERF4. Induced by salt stress and ABA. {ECO:0000269|PubMed:14731256, ECO:0000269|PubMed:16021341, ECO:0000269|PubMed:16998070, ECO:0000269|PubMed:18162593}. |
| Interaction | Q9LDI3:CIPK24; NbExp=4; IntAct=EBI-537680, EBI-537551; O81439:PAP1; NbExp=5; IntAct=EBI-537680, EBI-962363; Q84MC7:PYL9; NbExp=3; IntAct=EBI-537680, EBI-2349513; O49686:PYR1; NbExp=3; IntAct=EBI-537680, EBI-2349590; Q84JG2:SWI3B; NbExp=2; IntAct=EBI-537680, EBI-1102271; |
| Miscellaneous | The reduced form of ABI2 is converted to the oxidized form by the addition of oxidized GPX3 or H(2)O(2). |
| Similarity | Belongs to the PP2C family. {ECO:0000305}. |
| Similarity | Contains 1 PP2C-like domain. {ECO:0000305}. |
| Subunit | Interacts with CIPK15/PKS3, GPX3, SCAR1, SCAR2, SCAR3 and SCARL. Interacts also with CIPK24/SOS2. Binds to the fibrillin precursor protein. Interacts with ABA-bounded PYR1, PYL1, PYL2, PYL3, PYL4, PYL5, PYL6, PYL8 and PYL9, and with free PYL2, PYL3 and PYL4. {ECO:0000269|PubMed:12194854, ECO:0000269|PubMed:14504388, ECO:0000269|PubMed:16571665, ECO:0000269|PubMed:16998070, ECO:0000269|PubMed:17267444, ECO:0000269|PubMed:19407142, ECO:0000269|PubMed:19407143, ECO:0000269|PubMed:19769575}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP011133 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 15242022 | RefSeq | NP_200515 | 423 | protein phosphatase 2C 77 |
| 186532520 | RefSeq | NP_001119448 | 383 | protein phosphatase 2C 77 |
Identical Sequences to LMP011133 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15242022 | EMBL | CAW50797.1 | 423 | unnamed protein product [Arabidopsis thaliana] |
| GI:15242022 | EMBL | CAW54045.1 | 423 | unnamed protein product [Arabidopsis thaliana] |
| GI:15242022 | EMBL | CBC26694.1 | 423 | unnamed protein product [Arabidopsis thaliana] |
| GI:15242022 | EMBL | CBC20177.1 | 423 | unnamed protein product [Arabidopsis thaliana] |
| GI:15242022 | GenBank | AFC15992.1 | 423 | Sequence 6 from patent US 8115052 |
| GI:15242022 | gnl | TAIR | 423 | protein phosphatase 2C 77 [Arabidopsis thaliana] |
| GI:186532520 | gnl | TAIR | 383 | protein phosphatase 2C 77 [Arabidopsis thaliana] |
Related Sequences to LMP011133 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:186532520 | DBBJ | BAA97035.1 | 423 | protein phosphatase 2C ABI2 (PP2C) [Arabidopsis thaliana] |
| GI:186532520 | EMBL | CAA70163.1 | 423 | ABI2 protein phosphatase 2C [Arabidopsis thaliana] |
| GI:186532520 | EMBL | CAA72538.1 | 423 | ABI2 [Arabidopsis thaliana] |
| GI:186532520 | GenBank | AAP68299.1 | 423 | At5g57050 [Arabidopsis thaliana] |
| GI:15242022 | GenBank | EFH40754.1 | 433 | hypothetical protein ARALYDRAFT_495797 [Arabidopsis lyrata subsp. lyrata] |
| GI:15242022 | GenBank | EOA13392.1 | 434 | hypothetical protein CARUB_v10026433mg [Capsella rubella] |
| GI:186532520 | gnl | TAIR | 423 | protein phosphatase 2C 77 [Arabidopsis thaliana] |
| GI:15242022 | RefSeq | XP_002864495.1 | 433 | hypothetical protein ARALYDRAFT_495797 [Arabidopsis lyrata subsp. lyrata] |
| GI:15242022 | RefSeq | XP_006280494.1 | 434 | hypothetical protein CARUB_v10026433mg [Capsella rubella] |
| GI:15242022 | RefSeq | XP_010443374.1 | 496 | PREDICTED: protein phosphatase 2C 77-like [Camelina sativa] |
| GI:15242022 | RefSeq | XP_010483200.1 | 435 | PREDICTED: protein phosphatase 2C 77 [Camelina sativa] |
| GI:186532520 | SwissProt | O04719.1 | 423 | RecName: Full=Protein phosphatase 2C 77; Short=AtPP2C77; AltName: Full=Protein ABSCISIC ACID-INSENSITIVE 2; AltName: Full=Protein phosphatase 2C ABI2; Short=PP2C ABI2 [Arabidopsis thaliana] |