Gene/Proteome Database (LMPD)
LMPD ID
LMP011274
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
serine/threonine-protein kinase/endoribonuclease IRE1a
Gene Symbol
Synonyms
ARABIDOPSIS THALIANA INOSITOL REQUIRING 1-2; ATIRE1-2; INOSITOL REQUIRING 1-2; IRE1-2; IRE1A; MJB20.8; MJB20_8; TRANSMEMBRANE PROTEIN KINASE/RIBONUCLEASE
Alternate Names
serine/threonine-protein kinase/endoribonuclease IRE1a
Chromosome
2
EC Number
2.7.11.1
Summary
Encodes a endoribonuclease/protein kinase IRE1-like protein that is predicted to form a type I transmembrane protein structure and contain kinase/endoribonuclease domains at their C-terminal halves. The transcript levels for several ER-stress responsive genes, including six protein disulfide isomerases (PDIs), BiP2, and AtbZIP60 are not affected in ire1-2 null mutants.
Orthologs
Proteins
| serine/threonine-protein kinase/endoribonuclease IRE1a | |
|---|---|
| Refseq ID | NP_565419 |
| Protein GI | 18398454 |
| UniProt ID | Q9C5S2 |
| mRNA ID | NM_127306 |
| Length | 841 |
| RefSeq Status | REVIEWED |
| MPPRCPFLRHLFFLLLLLSPWIMSPCGGAADDVTYPIVPSSPGRRSILQIRREPPTEPNTKLVVDRDGKVFLKQQPKETPYWSFSTGSPMHSLYQAPANNNTENATEITRPHIIVEYLNNSKAATTVDGYHNWTVQEFFRQKPLVTDDGVTLGSETTSAYLVDGRSGRLIHVYKSTGDTKITNALVKPASTEDFVNEPLLIRRTDSKLEHFSKTTGKLVWNLTVSHFRAALLCDPVFNSGYDLGPKLQTGIYMPLLCGSQIDVRGPEIVIRVLHDQPMNVKMLPSPSLNHFESENSIMPFGKARESRKLQEQHKQKYTYLFGQWSPVKLLAPLVLLGVVVSVFIKKFSSRGSDVSLKAGPSKKKKNRKSAKDTNRQSVPRGQDQFELIEGGQMLLGFNNFQSGATDGRKIGKLFLSSKEIAKGSNGTVVFEGIYEGRPVAVKRLVRSHHEVAFKEIQNLIASDQHTNIIRWYGVEYDQDFVYLSLERCTCSLDDLIKSYLEFSMTKVLENNDSTEGVAAYKIQLDSLEGVIKGNNFWKVGGHPSPLMLKLMRDIVCGIVHLHELGIVHRDLKPQNVLISKDMTLSAKLSDMGISKRMSRDMSSLGHLATGSGSSGWQAPEQLLQGRQTRAVDMFSLGCVIFYTITGCKHPFGDDLERDVNIVKNKVDLFLVEHVPEASDLISRLLNPDPDLRPSATEVLLHPMFWNSEMRLSFLRDASDRVELENREADSEILKAMESTAPVAIGGKWDEKLEPVFITNIGRYRRYKYDSIRDLLRVIRNKLNHHRELPPEIQELVGTVPEGFDEYFAVRFPKLLIEVYRVISLHCREEEVFRKYFKCDII | |
Gene Information
Entrez Gene ID
Gene Name
serine/threonine-protein kinase/endoribonuclease IRE1a
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IDA:UniProtKB | C | endoplasmic reticulum |
| GO:0042406 | IDA:TAIR | C | extrinsic component of endoplasmic reticulum membrane |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0004521 | IMP:TAIR | F | endoribonuclease activity |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0004674 | IEA:UniProtKB-KW | F | protein serine/threonine kinase activity |
| GO:0090502 | IMP:GOC | P | RNA phosphodiester bond hydrolysis, endonucleolytic |
| GO:0008380 | IMP:UniProtKB | P | RNA splicing |
| GO:0006987 | IMP:TAIR | P | activation of signaling protein activity involved in unfolded protein response |
| GO:0009816 | IMP:UniProtKB | P | defense response to bacterium, incompatible interaction |
| GO:0030968 | IGI:TAIR | P | endoplasmic reticulum unfolded protein response |
| GO:0006397 | IEA:UniProtKB-KW | P | mRNA processing |
| GO:0046777 | IMP:UniProtKB | P | protein autophosphorylation |
| GO:0006355 | IEA:UniProtKB-KW | P | regulation of transcription, DNA-templated |
| GO:0009751 | IMP:UniProtKB | P | response to salicylic acid |
| GO:0006351 | IEA:UniProtKB-KW | P | transcription, DNA-templated |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath04141 | Protein processing in endoplasmic reticulum |
| ko04141 | Protein processing in endoplasmic reticulum |
REACTOME Pathway Links
Domain Information
UniProt Annotations
Entry Information
Gene Name
serine/threonine-protein kinase/endoribonuclease IRE1a
Protein Entry
IRE1A_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; |
| Disruption Phenotype | No visible phenotype but shows enhanced susceptibility to a bacterial pathogen and deficiency in establishing systemic acquired resistance (SAR). Ire1a and ire1b double mutant is more sensitive to the ER stress inducer tunicamycin than the wild-type and is enable to give rise to the spliced bZIP60 mRNA form (PubMed:22355548). Ire1a and ire1b double mutant displays short roots and a ER stress-sensitive phenotype (PubMed:21914012). {ECO:0000269|PubMed:21482766, ECO:0000269|PubMed:21914012, ECO:0000269|PubMed:22355548, ECO:0000269|PubMed:22359644}. |
| Enzyme Regulation | The kinase domain is activated by trans- autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain. |
| Function | Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices bZIP60 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator which then induces transcription of UPR target genes. Involved in organ growth regulation. Plays a role in plant immunity and abiotic stress responses. {ECO:0000269|PubMed:11706177, ECO:0000269|PubMed:12020828, ECO:0000269|PubMed:21914012, ECO:0000269|PubMed:22355548, ECO:0000269|PubMed:22359644}. |
| Induction | By ER stress inducer tunicamycin, by salicylic acid (SA) and by bacterial pathogen infection. |
| Ptm | Autophosphorylated. |
| Similarity | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}. |
| Similarity | Contains 1 KEN domain. {ECO:0000255|PROSITE- ProRule:PRU00725}. |
| Similarity | Contains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}. |
| Subcellular Location | Endoplasmic reticulum membrane {ECO:0000269|PubMed:11706177}; Single-pass type I membrane protein {ECO:0000269|PubMed:11706177}. |
| Subunit | Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges (By similarity). {ECO:0000250}. |
| Tissue Specificity | Ubiquitous. Detected in the vascular bundles of young plants, leaves, roots, seedlings and in the receptacles of flowers and vascular bundles of the petals. {ECO:0000269|PubMed:11706177, ECO:0000269|PubMed:12020828}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP011274 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 18398454 | RefSeq | NP_565419 | 841 | serine/threonine-protein kinase/endoribonuclease IRE1a |
Identical Sequences to LMP011274 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:18398454 | DBBJ | BAB63367.1 | 841 | Ire1 homolog-2 [Arabidopsis thaliana] |
| GI:18398454 | GenBank | AAK15470.1 | 841 | endoribonuclease/protein kinase IRE1-like protein [Arabidopsis thaliana] |
| GI:18398454 | GenBank | AEC06640.1 | 841 | endoribonuclease/protein kinase IRE1-like protein [Arabidopsis thaliana] |
| GI:18398454 | gnl | TIGR | 841 | putative protein kinase/endoribonuclease [Arabidopsis thaliana] |
| GI:18398454 | SwissProt | Q9C5S2.1 | 841 | RecName: Full=Serine/threonine-protein kinase/endoribonuclease IRE1a; AltName: Full=Endoplasmic reticulum-to-nucleus signaling 1-2; AltName: Full=Inositol-requiring protein 1-2; Short=AtIRE1-2; AltName: Full=Serine/threonine-protein kinase/endoribonuclease IRE1-2; Includes: RecName: Full=Serine/threonine-protein kinase; Includes: RecName: Full=Endoribonuclease; Flags: Precursor [Arabidopsis thaliana] |
Related Sequences to LMP011274 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:18398454 | DBBJ | BAF00868.1 | 841 | endoribonuclease/protein kinase IRE1 [Arabidopsis thaliana] |
| GI:18398454 | GenBank | EFH60322.1 | 839 | hypothetical protein ARALYDRAFT_480637 [Arabidopsis lyrata subsp. lyrata] |
| GI:18398454 | RefSeq | XP_002884063.1 | 839 | hypothetical protein ARALYDRAFT_480637 [Arabidopsis lyrata subsp. lyrata] |
| GI:18398454 | RefSeq | XP_010514917.1 | 853 | PREDICTED: serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Camelina sativa] |
| GI:18398454 | RefSeq | XP_010514924.1 | 853 | PREDICTED: serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 [Camelina sativa] |
| GI:18398454 | RefSeq | XP_010514931.1 | 853 | PREDICTED: serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 [Camelina sativa] |