Gene/Proteome Database (LMPD)

LMPD ID
LMP011292
Gene ID
843513
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
somatic embryogenesis receptor kinase 1
Gene Symbol
SERK1
Synonyms
ATSERK1; F14O23.21; F14O23_21; SERK1; somatic embryogenesis receptor-like kinase 1; SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 1
Alternate Names
somatic embryogenesis receptor kinase 1
Chromosome
1
EC Number
2.7.10.1
Summary
Plasma membrane LRR receptor-like serine threonine kinase expressed during embryogenesis in locules until stage 6 anthers, with higher expression in the tapetal cell layer. SERK1 and SERK2 receptor kinases function redundantly as an important control point for sporophytic development controlling male gametophyte production. later
Orthologs
View orthologs and multiple alignments for SERK1

Proteins

somatic embryogenesis receptor kinase 1
Refseq ID NP_177328
Protein GI 15217584
UniProt ID Q94AG2
mRNA ID NM_105841
Length 625
RefSeq Status REVIEWED
MESSYVVFILLSLILLPNHSLWLASANLEGDALHTLRVTLVDPNNVLQSWDPTLVNPCTWFHVTCNNENSVIRVDLGNAELSGHLVPELGVLKNLQYLELYSNNITGPIPSNLGNLTNLVSLDLYLNSFSGPIPESLGKLSKLRFLRLNNNSLTGSIPMSLTNITTLQVLDLSNNRLSGSVPDNGSFSLFTPISFANNLDLCGPVTSHPCPGSPPFSPPPPFIQPPPVSTPSGYGITGAIAGGVAAGAALLFAAPAIAFAWWRRRKPLDIFFDVPAEEDPEVHLGQLKRFSLRELQVASDGFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPPSQPPLDWPTRKRIALGSARGLSYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGIMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEMLVDPDLQTNYEERELEQVIQVALLCTQGSPMERPKMSEVVRMLEGDGLAEKWDEWQKVEILREEIDLSPNPNSDWILDSTYNLHAVELSGPR

Gene Information

Entrez Gene ID
843513
Gene Name
somatic embryogenesis receptor kinase 1
Gene Symbol
SERK1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005886 IDA:UniProtKB C plasma membrane
GO:0043234 IPI:TAIR C protein complex
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0042802 IPI:IntAct F identical protein binding
GO:0033612 IPI:UniProtKB F receptor serine/threonine kinase binding
GO:0004675 IDA:TAIR F transmembrane receptor protein serine/threonine kinase activity
GO:0004714 IEA:UniProtKB-EC F transmembrane receptor protein tyrosine kinase activity
GO:0007030 IGI:TAIR P Golgi organization
GO:0009742 IMP:TAIR P brassinosteroid mediated signaling pathway
GO:0009793 IMP:TAIR P embryo development ending in seed dormancy
GO:0010227 IGI:TAIR P floral organ abscission
GO:0009556 IMP:TAIR P microsporogenesis
GO:0010152 IMP:TAIR P pollen maturation
GO:0046777 TAS:TAIR P protein autophosphorylation
GO:0006468 IDA:TAIR P protein phosphorylation
GO:0007178 IDA:GOC P transmembrane receptor protein serine/threonine kinase signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR013320 Concanavalin A-like lectin/glucanase domain
IPR025875 Leucine rich repeat 4
IPR001611 Leucine-rich repeat
IPR013210 Leucine-rich repeat-containing N-terminal, type 2
IPR000719 Protein kinase domain
IPR017441 Protein kinase, ATP binding site
IPR011009 Protein kinase-like domain
IPR008271 Serine/threonine-protein kinase, active site

UniProt Annotations

Entry Information

Gene Name
somatic embryogenesis receptor kinase 1
Protein Entry
SERK1_ARATH
UniProt ID
Q94AG2
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=4 uM for ATP {ECO:0000269|PubMed:11509554};
Catalytic Activity ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. {ECO:0000255|PROSITE- ProRule:PRU10027}.
Catalytic Activity ATP + a protein = ADP + a phosphoprotein.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Developmental Stage Expressed during pollen development and megasporogenesis in the nucellus of developing ovules, in all cells of the embryo sac up to fertilization and in all cells of the developing embryo until the heart-shaped stage. Found in epidermal and vascular cells of the late torpedo and cotyledon stages embryos. {ECO:0000269|PubMed:11706164, ECO:0000269|PubMed:16231101, ECO:0000269|PubMed:18515128}.
Disruption Phenotype No visible phenotype. Serk1 and serk2 double mutants are completely male sterile due to a failure in tapetum specification. {ECO:0000269|PubMed:16284305, ECO:0000269|PubMed:16284306}.
Domain The extracellular domain (26-234) is required for dimerization.
Enzyme Regulation Inhibited by manganese.
Function Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Phosphorylates BRI1 on 'Ser- 887' and CDC48 on at least one threonine residue and on 'Ser-41'. Confers embryogenic competence. Acts redundantly with SERK2 as a control point for sporophytic development controlling male gametophyte production. Involved in the brassinolide signaling pathway. {ECO:0000269|PubMed:11509554, ECO:0000269|PubMed:17693538}.
Interaction Self; NbExp=3; IntAct=EBI-1555537, EBI-1555537; O22476:BRI1; NbExp=5; IntAct=EBI-1555537, EBI-1797828; P54609:CDC48A; NbExp=12; IntAct=EBI-1555537, EBI-1563238; P48349:GRF6; NbExp=6; IntAct=EBI-1555537, EBI-1633785;
Miscellaneous Seems to be related with early development of tissues in general rather than with embryogenesis.
Ptm Glycosylated. Important for targeting to the plasma membrane.
Ptm Intermolecular autophosphorylation. The catalytic activity of SERK1 depends on the presence of a phosphorylated Thr residue in SERK1. The phosphorylation is induced by brassinosteroids. Transphosphorylation by BRI1 occurs only on Ser-299 and Thr-462. Dephosphorylation of threonine residues by the kinase-associated protein phosphatase (KAPP) is involved in SERK1 endocytosis. {ECO:0000269|PubMed:19105183}.
Sequence Caution Sequence=AAF43236.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
Similarity Contains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}.
Similarity Contains 4 LRR (leucine-rich) repeats. {ECO:0000305}.
Subcellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Subunit Monomer, homo- and heterodimer. Interacts with KAPP, CDC48A, GRF6 or GRF7, SERK2, BRI1 and SERK3/BAK1 to form the SERK1 signaling complex. Interacts with the EF-Tu receptor EFR and FLS2 in a specific ligand-induced manner. {ECO:0000269|PubMed:11397085, ECO:0000269|PubMed:12101128, ECO:0000269|PubMed:15592873, ECO:0000269|PubMed:16284305, ECO:0000269|PubMed:16473966, ECO:0000269|PubMed:16621602, ECO:0000269|PubMed:17693538, ECO:0000269|PubMed:17905839, ECO:0000269|PubMed:21693696}.
Tissue Specificity Expressed in flowers, tapetum, developing microspores, all cells of the embryo sac, provascular strands and developing vascular bundles. Low expression in adult vascular tissue. Detected in root meristem. {ECO:0000269|PubMed:11706164, ECO:0000269|PubMed:16231101, ECO:0000269|PubMed:16284305, ECO:0000269|PubMed:18515128}.
Web Resource Name=PlantP kinase Classification PPC; URL="http://plantsp.genomics.purdue.edu/family/class.html";

Identical and Related Proteins

Unique RefSeq proteins for LMP011292 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15217584 RefSeq NP_177328 625 somatic embryogenesis receptor kinase 1

Identical Sequences to LMP011292 proteins

Reference Database Accession Length Protein Name
GI:15217584 EMBL CBA12744.1 625 unnamed protein product [Arabidopsis thaliana]
GI:15217584 EMBL CBD14098.1 625 unnamed protein product [Arabidopsis thaliana]
GI:15217584 GenBank ACN59271.1 625 leucine-rich repeat receptor-like protein kinase, partial [Arabidopsis thaliana]
GI:15217584 GenBank AEE35238.1 625 somatic embryogenesis receptor kinase 1 [Arabidopsis thaliana]
GI:15217584 GenBank AEH74993.1 625 Sequence 25 from patent US 7956240
GI:15217584 SwissProt Q94AG2.2 625 RecName: Full=Somatic embryogenesis receptor kinase 1; Short=AtSERK1; AltName: Full=Somatic embryogenesis receptor-like kinase 1; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP011292 proteins

Reference Database Accession Length Protein Name
GI:15217584 EMBL CAX06439.1 625 unnamed protein product [Arabidopsis thaliana]
GI:15217584 EMBL CBA12777.1 625 unnamed protein product [Arabidopsis thaliana]
GI:15217584 EMBL CBD14170.1 625 unnamed protein product [Arabidopsis thaliana]
GI:15217584 GenBank AAK82463.1 625 At1g71830/F14O23_24 [Arabidopsis thaliana]
GI:15217584 GenBank AAN72307.1 625 At1g71830/F14O23_24 [Arabidopsis thaliana]
GI:15217584 GenBank AHL57119.1 625 Sequence 2 from patent US 8642837