LIPID MAPS

LMPD ID

LMP012372

Gene ID

201164

Species

Homo sapiens (Human)

Gene Name

phospholipase D family, member 6

Gene Symbol

PLD6

Synonyms

ZUC;

Chromosome

17

Map Location

17p11.2

EC Number

3.1.-.-

mitochondrial cardiolipin hydrolase
Refseq ID	NP_849158
Protein GI	270265812
UniProt ID	Q8N2A8
mRNA ID	NM_178836
Length	252
MGRLSWQVAAAAAVGLALTLEALPWVLRWLRSRRRRPRREALFFPSQVTCTEALLRAPGAELAELPEGCPCGLPHGESALSRLLRALLAARASLDLCLFAFSSPQLGRAVQLLHQRGVRVRVVTDCDYMALNGSQIGLLRKAGIQVRHDQDPGYMHHKFAIVDKRVLITGSLNWTTQAIQNNRENVLITEDDEYVRLFLEEFERIWEQFNPTKYTFFPPKKSHGSCAPPVSRAGGRLLSWHRTCGTSSESQT

Entrez Gene ID

201164

Gene Name

phospholipase D family, member 6

Gene Symbol

PLD6

Species

Homo sapiens

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0005741	IDA:UniProtKB	C	mitochondrial outer membrane
GO:0035755	IDA:UniProtKB	F	cardiolipin hydrolase activity
GO:0004519	IEA:UniProtKB-KW	F	endonuclease activity
GO:0046872	IEA:UniProtKB-KW	F	metal ion binding
GO:0042803	IDA:UniProtKB	F	protein homodimerization activity
GO:0043046	ISS:UniProtKB	P	DNA methylation involved in gamete generation
GO:0030719	ISS:UniProtKB	P	P granule organization
GO:0046474	TAS:Reactome	P	glycerophospholipid biosynthetic process
GO:0016042	IEA:UniProtKB-KW	P	lipid catabolic process
GO:0007126	ISS:UniProtKB	P	meiotic nuclear division
GO:0008053	IMP:UniProtKB	P	mitochondrial fusion
GO:0006655	TAS:Reactome	P	phosphatidylglycerol biosynthetic process
GO:0006644	TAS:Reactome	P	phospholipid metabolic process
GO:0034587	ISS:UniProtKB	P	piRNA metabolic process
GO:0044281	TAS:Reactome	P	small molecule metabolic process
GO:0007286	ISS:UniProtKB	P	spermatid development

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY-3561	choline biosynthesis III
LIPASYN-PWY	phospholipases

REACTOME Pathway Links

REACTOME Pathway ID	Description
REACT_121401	Glycerophospholipid biosynthesis
REACT_111217	Metabolism
REACT_22258	Metabolism of lipids and lipoproteins
REACT_120870	Phospholipid metabolism
REACT_121280	Synthesis of PG

InterPro Annotations

Accession	Description
IPR025202	Phospholipase D-like domain
IPR001736	Phospholipase D/Transphosphatidylase

Entry Information

Gene Name

phospholipase D family, member 6

UniProt ID

Q8N2A8

Species

Human

Comments

Comment Type	Description
Domain	In contrast to other members of the phospholipase D family, contains only one PLD phosphodiesterase domain, suggesting that it has a single half-catalytic and requires homodimerization to form a complete active site
Enzyme Regulation	SsDNA hydrolase activity does not depend upon, but it stimulated by, the presence of Ca(2+) and Mn(2+)
Function	Regulates mitochondrial shape through facilitating mitochondrial fusion. During spermatogenesis, plays a critical role in PIWI-interacting RNA (piRNA) biogenesis (By similarity). piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity (By similarity). Produces 5' phosphate and 3' hydroxyl termini, suggesting it could directly participate in the processing of primary piRNA transcripts (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface. Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low (PubMed:21397848). In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol. {ECO:0000250, ECO:0000269\|PubMed:17028579, ECO:0000269\|PubMed:21397848}.
Similarity	Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily
Similarity	Contains 1 C3H1-type zinc finger
Similarity	Contains 1 PLD phosphodiesterase domain
Subcellular Location	Mitochondrion outer membrane ; Single-pass membrane protein
Subunit	Homodimer
Tissue Specificity	Widely expressed. Expression is not limited to testis and ovary. {ECO:0000269\|PubMed:17028579, ECO:0000269\|PubMed:21397847}.