Gene/Proteome Database (LMPD)

LMPD ID
LMP012532
Gene ID
25596
Species
Rattus norvegicus (Rat)
Gene Name
ribophorin I
Gene Symbol
Rpn1
Synonyms
RIBI;
Alternate Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; RPN-I; ribophorin1; ribophorin-1; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit;
Chromosome
4
Map Location
chromosome:4
EC Number
2.4.99.18
Summary
may play a role in translocation of nascent polypeptides synthesized on membrane bound polysomes [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Rpn1

Proteins

dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 precursor
Refseq ID NP_037199
Protein GI 6981486
UniProt ID P07153
mRNA ID NM_013067
Length 605
MEAPIVLLLLLWLALAPTPGSASSEAPPLVNEDVKRTVDLSSHLAKVTAEVVLAHPGGGSTARASSFVLALEPELESRLAHLGVQVKGEDEEDNNLEVRETKMKGKSGRFFTVKLPVALDPGSKISIVVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVRLASRNVESHTKLGNPSRSEDILDYGPFKDIPAYSQDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFGLFGGWKTHYIVGYNLPSYEYLYNLGDQYALKMRFVDHVFDEQVIDSLTVKIILPEGAKNIQVDSPYDISRAPDELHYTYLDTFGRPVIVAYKKNLVEQHIQDIVVHYTFNKVLMLQEPLLVVAAFYILFFTVIIYVRLDFSITKDPAAEARMKVACITEQVLTLVNKRLGLYRHFDETVNRYKQSRDISTLNSGKKSLETEHKAVTSEIAVLQSRLKTEGSDLCDRVSEMQKLDAQVKELVLKSAVEAERLVAGKLKKDTYIENEKLSSGKRQELVTKIDHILDAL
sig_peptide: 1..22 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2290 peptide sequence: MEAPIVLLLLLWLALAPTPGSA mat_peptide: 23..605 product: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 calculated_mol_wt: 66033 peptide sequence: SSEAPPLVNEDVKRTVDLSSHLAKVTAEVVLAHPGGGSTARASSFVLALEPELESRLAHLGVQVKGEDEEDNNLEVRETKMKGKSGRFFTVKLPVALDPGSKISIVVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVRLASRNVESHTKLGNPSRSEDILDYGPFKDIPAYSQDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFGLFGGWKTHYIVGYNLPSYEYLYNLGDQYALKMRFVDHVFDEQVIDSLTVKIILPEGAKNIQVDSPYDISRAPDELHYTYLDTFGRPVIVAYKKNLVEQHIQDIVVHYTFNKVLMLQEPLLVVAAFYILFFTVIIYVRLDFSITKDPAAEARMKVACITEQVLTLVNKRLGLYRHFDETVNRYKQSRDISTLNSGKKSLETEHKAVTSEIAVLQSRLKTEGSDLCDRVSEMQKLDAQVKELVLKSAVEAERLVAGKLKKDTYIENEKLSSGKRQELVTKIDHILDAL

Gene Information

Entrez Gene ID
25596
Gene Name
ribophorin I
Gene Symbol
Rpn1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005791 IDA:RGD C rough endoplasmic reticulum
GO:0004579 IEA:InterPro F dolichyl-diphosphooligosaccharide-protein glycotransferase activity
GO:0015833 NAS:RGD P peptide transport
GO:0006486 IEA:UniProtKB-UniPathway P protein glycosylation

KEGG Pathway Links

KEGG Pathway ID Description
rno01100 Metabolic pathways
ko00510 N-Glycan biosynthesis
rno00510 N-Glycan biosynthesis
M00072 N-glycosylation by oligosaccharyltransferase
ko04141 Protein processing in endoplasmic reticulum
rno04141 Protein processing in endoplasmic reticulum

Domain Information

InterPro Annotations

Accession Description
IPR007676 Ribophorin_I

UniProt Annotations

Entry Information

Gene Name
ribophorin I
Protein Entry
RPN1_RAT
UniProt ID
P07153
Species
Rat

Comments

Comment Type Description
Catalytic Activity Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
Function Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Pathway Protein modification; protein glycosylation.
Similarity Belongs to the OST1 family
Subcellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome .
Subunit Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex (By similarity)
Tissue Specificity Expressed in all tissues tested.

Identical and Related Proteins

Unique RefSeq proteins for LMP012532 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6981486 RefSeq NP_037199 605 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 precursor

Identical Sequences to LMP012532 proteins

Reference Database Accession Length Protein Name
GI:6981486 EMBL CAA28919.1 605 ribophorin I [Rattus norvegicus]
GI:6981486 SwissProt P07153.1 605 RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; AltName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; AltName: Full=Ribophorin I; Short=RPN-I; AltName: Full=Ribophorin-1; Flags: Precursor [Rattus norvegicus]

Related Sequences to LMP012532 proteins

Reference Database Accession Length Protein Name
GI:6981486 EMBL CAA28919.1 605 ribophorin I [Rattus norvegicus]
GI:6981486 GenBank EDL91306.1 606 ribophorin I, isoform CRA_b [Rattus norvegicus]
GI:6981486 SwissProt P07153.1 605 RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; AltName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; AltName: Full=Ribophorin I; Short=RPN-I; AltName: Full=Ribophorin-1; Flags: Precursor [Rattus norvegicus]