Gene/Proteome Database (LMPD)
LMPD ID
LMP012563
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
O-linked N-acetylglucosamine (GlcNAc) transferase
Gene Symbol
Alternate Names
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase subunit p110; O linked N-acetylglucosamine transferase; O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase);
Chromosome
X
Map Location
Xq31
EC Number
2.4.1.255
Summary
the catalytic p110 subunit of the enzyme involved in the dynamic O-GlcNAcylation of nuclear and cytosolic proteins [RGD, Feb 2006]
Orthologs
Proteins
| UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit | |
|---|---|
| Refseq ID | NP_058803 |
| Protein GI | 139948535 |
| UniProt ID | P56558 |
| mRNA ID | NM_017107 |
| Length | 1036 |
| MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVAEQLEKNRLPSVHPHHSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNADTTNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKSRQEYEDIAVKLGTDLEYLKKIRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPVEVTESA | |
Gene Information
Entrez Gene ID
Gene Name
O-linked N-acetylglucosamine (GlcNAc) transferase
Gene Symbol
Species
Rattus norvegicus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | IDA:RGD | C | cytosol |
| GO:0000123 | ISS:UniProtKB | C | histone acetyltransferase complex |
| GO:0005634 | IDA:RGD | C | nucleus |
| GO:0005886 | ISS:UniProtKB | C | plasma membrane |
| GO:0048029 | IDA:RGD | F | monosaccharide binding |
| GO:0042277 | IDA:RGD | F | peptide binding |
| GO:0005547 | ISS:UniProtKB | F | phosphatidylinositol-3,4,5-trisphosphate binding |
| GO:0016262 | IDA:RGD | F | protein N-acetylglucosaminyltransferase activity |
| GO:0097363 | ISS:UniProtKB | F | protein O-GlcNAc transferase activity |
| GO:0006915 | ISS:UniProtKB | P | apoptotic process |
| GO:0032922 | ISS:UniProtKB | P | circadian regulation of gene expression |
| GO:0080182 | ISS:UniProtKB | P | histone H3-K4 trimethylation |
| GO:0043984 | ISS:UniProtKB | P | histone H4-K16 acetylation |
| GO:0043981 | ISS:UniProtKB | P | histone H4-K5 acetylation |
| GO:0043982 | ISS:UniProtKB | P | histone H4-K8 acetylation |
| GO:0031397 | ISS:UniProtKB | P | negative regulation of protein ubiquitination |
| GO:0048015 | ISS:UniProtKB | P | phosphatidylinositol-mediated signaling |
| GO:0061087 | ISS:UniProtKB | P | positive regulation of histone H3-K27 methylation |
| GO:0045862 | ISS:UniProtKB | P | positive regulation of proteolysis |
| GO:0045944 | ISS:UniProtKB | P | positive regulation of transcription from RNA polymerase II promoter |
| GO:0006493 | IDA:RGD | P | protein O-linked glycosylation |
| GO:0070207 | IDA:RGD | P | protein homotrimerization |
| GO:0090526 | ISS:UniProtKB | P | regulation of gluconeogenesis involved in cellular glucose homeostasis |
| GO:0006110 | ISS:UniProtKB | P | regulation of glycolytic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko00514 | Other types of O-glycan biosynthesis |
| rno00514 | Other types of O-glycan biosynthesis |
REACTOME Pathway Links
Domain Information
UniProt Annotations
Entry Information
Gene Name
O-linked N-acetylglucosamine (GlcNAc) transferase
Protein Entry
OGT1_RAT
UniProt ID
Species
Rat
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | UDP-N-acetyl-D-glucosamine + [protein]-L- serine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine. |
| Catalytic Activity | UDP-N-acetyl-D-glucosamine + [protein]-L- threonine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L- threonine. |
| Domain | The TPR repeat domain is required for substrate binding and oligomerization. |
| Function | Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta- linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O- GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues. O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex. Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O- glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2 (By similarity) |
| Interaction | Q9WVH3:Foxo4 (xeno); NbExp=2; IntAct=EBI-7614183, EBI-4567305; |
| Pathway | Protein modification; protein glycosylation. |
| Ptm | O-glycosylated; contains O-GlcNAc |
| Ptm | Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity |
| Ptm | Ubiquitinated, leading to its proteasomal degradation |
| Similarity | Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily |
| Similarity | Contains 13 TPR repeats. {ECO:0000255|PROSITE- ProRule:PRU00339}. |
| Subcellular Location | Cytoplasm. Nucleus. Cell membrane. Note=Mostly in the nucleus. Retained in the nucleus via interaction with HCFC1. After insulin induction, translocated from the nucleus to the cell membrane via phophatidylinisotide binding. Colocalizes with AKT1 at the plasma membrane (By similarity) |
| Subunit | Heterotrimer; consists of one 78 kDa subunit and two 110 kDa subunits dimerized via TPR repeats 6 and 7. Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, HCFC1, PPP1CC and ACTB. Component of a THAP1/THAP3-HCFC1- OGT complex. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts directly with HCFC1; the interaction O- glycosylates HCFC1, regulates its proteolytic processing and transcriptional activity and, in turn, stabilizes OGT in the nucleus. Interacts (via TPRs 1-6) with SIN3A; the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts (via TPR 5-6) with TET1, TET2 and TET3 (By similarity). Interacts (via TPR repeats 6 and 7) with ATXN10. Interacts with ARNTL/BMAL1 (By similarity) |
| Tissue Specificity | Expressed in brain, heart, liver, thymus, lung, pancreas, spleen, uterus and ovary. In the pancreas, expressed in both exocrine acinar cells and in endocrine cells of the islets of Langerhans. {ECO:0000269|PubMed:10580430, ECO:0000269|PubMed:16714295, ECO:0000269|PubMed:9083067}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP012563 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 139948535 | RefSeq | NP_058803 | 1036 | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit |
Identical Sequences to LMP012563 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:139948535 | GenBank | EDL95887.1 | 1036 | O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), isoform CRA_b [Rattus norvegicus] |
| GI:139948535 | GenBank | EDL95888.1 | 1036 | O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), isoform CRA_b [Rattus norvegicus] |
| GI:139948535 | RefSeq | XP_006257120.1 | 1036 | PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X1 [Rattus norvegicus] |
Related Sequences to LMP012563 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:139948535 | GenBank | EDL95887.1 | 1036 | O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), isoform CRA_b [Rattus norvegicus] |
| GI:139948535 | GenBank | EDL95888.1 | 1036 | O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), isoform CRA_b [Rattus norvegicus] |
| GI:139948535 | RefSeq | XP_006257120.1 | 1036 | PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X1 [Rattus norvegicus] |