Gene/Proteome Database (LMPD)

LMPD ID
LMP012678
Gene ID
60581
Species
Rattus norvegicus (Rat)
Gene Name
acetyl-CoA carboxylase alpha
Gene Symbol
Acaca
Synonyms
ACC1; Acac;
Alternate Names
acetyl-CoA carboxylase 1; ACC-alpha; acetyl-coenzyme A carboxylase alpha;
Chromosome
10
Map Location
10q26
EC Number
6.4.1.2
Summary
catalyzes the rate-limiting carboxylation of acetyl CoA to malonyl CoA during long-chain fatty acid synthesis [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Acaca

Proteins

acetyl-CoA carboxylase 1
Refseq ID NP_071529
Protein GI 11559962
UniProt ID P11497
mRNA ID NM_022193
Length 2345
MDEPSPLAKTLELNQHSRFIIGSVSEDNSEDEISNLVKLDLEEKEGSLSPASVSSDTLSDLGISALQDGLAFHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGANNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNVPQDLYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLFRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVNLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYEGIKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPQIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPLNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYDVRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFASNLNHYGMTHVASVSDVLLDNAFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPESGHTSLYDEDKVPRDEPIHILNVAIKTDGDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTTTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDILTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMSLKSPEYPDGRDVIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVAANSGARIGLAEEIRHMFHVAWVDSEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSYMPKNVHSSVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSVPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECSQPVMVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRKKDLVKTMRRVDPVYIRLAERLGTPELSPTERKELESKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHSANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Gene Information

Entrez Gene ID
60581
Gene Name
acetyl-CoA carboxylase alpha
Gene Symbol
Acaca
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 ISS:UniProtKB C cytosol
GO:0003989 IDA:RGD F acetyl-CoA carboxylase activity
GO:0005524 IDA:RGD F ATP binding
GO:0009374 IMP:RGD F biotin binding
GO:0004075 IEA:UniProtKB-EC F biotin carboxylase activity
GO:0019900 IPI:RGD F kinase binding
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0006084 IDA:RGD P acetyl-CoA metabolic process
GO:0006633 IDA:RGD P fatty acid biosynthetic process
GO:2001295 IEA:UniProtKB-UniPathway P malonyl-CoA biosynthetic process
GO:0051289 ISS:UniProtKB P protein homotetramerization
GO:0042493 IEP:RGD P response to drug
GO:0014070 IMP:RGD P response to organic cyclic compound

KEGG Pathway Links

KEGG Pathway ID Description
ko04152 AMPK signaling pathway
rno04152 AMPK signaling pathway
ko00061 Fatty acid biosynthesis
rno00061 Fatty acid biosynthesis
M00082 Fatty acid biosynthesis, initiation
ko01212 Fatty acid metabolism
rno01212 Fatty acid metabolism
ko04910 Insulin signaling pathway
rno04910 Insulin signaling pathway
rno01100 Metabolic pathways
ko00640 Propanoate metabolism
rno00640 Propanoate metabolism
ko00620 Pyruvate metabolism
rno00620 Pyruvate metabolism

Domain Information

InterPro Annotations

Accession Description
IPR013537 Acetyl-CoA carboxylase, central domain
IPR011763 Acetyl-coenzyme A carboxyltransferase, C-terminal
IPR011762 Acetyl-coenzyme A carboxyltransferase, N-terminal
IPR011761 ATP-grasp fold
IPR013815 ATP-grasp fold, subdomain 1
IPR013816 ATP-grasp fold, subdomain 2
IPR001882 Biotin-binding site
IPR005482 Biotin carboxylase, C-terminal
IPR011764 Biotin carboxylation domain
IPR000089 Biotin/lipoyl attachment
IPR005481 Carbamoyl-phosphate synthase, large subunit, N-terminal
IPR005479 Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain
IPR000022 Carboxyl transferase
IPR029045 ClpP/crotonase-like domain
IPR016185 Pre-ATP-grasp domain
IPR011054 Rudiment single hybrid motif
IPR011053 Single hybrid motif

UniProt Annotations

Entry Information

Gene Name
acetyl-CoA carboxylase alpha
Protein Entry
ACACA_RAT
UniProt ID
P11497
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P11497-1; Sequence=Displayed; Name=2; IsoId=P11497-2; Sequence=VSP_011753;
Catalytic Activity ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA.
Catalytic Activity ATP + biotin-[carboxyl-carrier-protein] + CO(2) = ADP + phosphate + carboxy-biotin-[carboxyl-carrier- protein].
Cofactor Name=biotin; Xref=ChEBI:CHEBI:57586; Note=Biotin.;
Cofactor Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence= ; Note=Binds 2 manganese ions per subunit. ;
Enzyme Regulation Activity is increased by oligomerization. Citrate and MID1IP1 promote oligomerization (By similarity). Activity is increased by phosphorylation
Function Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
Pathway Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
Ptm Phosphorylation at Ser-79 by AMPK inactivates enzyme activity. Phosphorylated in vitro at Ser-1200 and Ser-1215 by AMPK; the relevance of phosphorylation of these sites in vivo is however unclear. {ECO:0000269|PubMed:1346520, ECO:0000269|PubMed:1974251, ECO:0000269|PubMed:2900138, ECO:0000269|PubMed:9029219}.
Ptm Phosphorylation on Ser-1262 is required for interaction with BRCA1
Ptm The N-terminus is blocked.
Similarity Contains 1 ATP-grasp domain. {ECO:0000255|PROSITE- ProRule:PRU00409}.
Similarity Contains 1 biotin carboxylation domain
Similarity Contains 1 biotinyl-binding domain
Similarity Contains 1 carboxyltransferase domain
Subcellular Location Cytoplasm.
Subunit Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity (By similarity)

Identical and Related Proteins

Unique RefSeq proteins for LMP012678 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
11559962 RefSeq NP_071529 2345 acetyl-CoA carboxylase 1

Identical Sequences to LMP012678 proteins

Reference Database Accession Length Protein Name
GI:11559962 GenBank AGX53672.1 2345 Sequence 7694 from patent US 8541208
GI:11559962 GenBank AGX55939.1 2345 Sequence 12204 from patent US 8541208
GI:11559962 GenBank AGX60354.1 2345 Sequence 20939 from patent US 8541208
GI:11559962 GenBank AGX76987.1 2345 Sequence 53748 from patent US 8541208

Related Sequences to LMP012678 proteins

Reference Database Accession Length Protein Name
GI:11559962 EMBL CBF64062.1 2345 unnamed protein product [Rattus norvegicus]
GI:11559962 EMBL CBF60000.1 2345 unnamed protein product [Rattus norvegicus]
GI:11559962 EMBL CBF60635.1 2345 unnamed protein product [Rattus norvegicus]