Gene/Proteome Database (LMPD)
LMPD ID
LMP012728
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
radical S-adenosyl methionine domain containing 2
Gene Symbol
Synonyms
Vig1; Best5;
Alternate Names
radical S-adenosyl methionine domain-containing protein 2; viperin; rsad2 {ECO:0000250|UniProtKB:Q8WXG1}; bone-expressed sequence tag 5 protein; virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible;
Chromosome
6
Map Location
6q16
Summary
protein involved in bone formation [RGD, Feb 2006]
Orthologs
Proteins
| radical S-adenosyl methionine domain-containing protein 2 | |
|---|---|
| Refseq ID | NP_620236 |
| Protein GI | 20302057 |
| UniProt ID | O70600 |
| mRNA ID | NM_138881 |
| Length | 360 |
| MLVPTALAARLLSLFQQQLGSLWSGLAMLFCWLRIALGWPDPGKGQPRVRGEPKETQETHEDPGSAQPTTPVSVNYHFTRQCNYKCGFCFHTAKTSFVLPLEEAKRGLLLLKQAGMEKINFSGGEPFLQDRGEYLGKLVRFCKEELALPSVSIVSNGSLIRERWFKDYGDYLDILAISCDSFDEQVNVLIGRGQGKKNHVENLQKLRKWCRDYKVAFKINSVINRFNVDEDMNEHIKALSPVRWKVFQCLLIEGENSGEDALREAERFLISNEEFEAFLQRHKDVSCLVPESNQKMKDSYLILDEYMRFLNCTGGRKDPSRSILDVGVEEAIKFSGFDEKMFLKRGGKYVWSKADLKLDW | |
Gene Information
Entrez Gene ID
Gene Name
radical S-adenosyl methionine domain containing 2
Gene Symbol
Species
Rattus norvegicus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | ISS:UniProtKB | C | endoplasmic reticulum |
| GO:0005789 | ISS:UniProtKB | C | endoplasmic reticulum membrane |
| GO:0005811 | ISS:UniProtKB | C | lipid particle |
| GO:0005743 | IEA:Ensembl | C | mitochondrial inner membrane |
| GO:0005741 | IEA:Ensembl | C | mitochondrial outer membrane |
| GO:0051539 | ISS:UniProtKB | F | 4 iron, 4 sulfur cluster binding |
| GO:0003824 | IEA:InterPro | F | catalytic activity |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0043621 | ISS:UniProtKB | F | protein self-association |
| GO:0035710 | ISS:UniProtKB | P | CD4-positive, alpha-beta T cell activation |
| GO:0043367 | ISS:UniProtKB | P | CD4-positive, alpha-beta T cell differentiation |
| GO:0051607 | ISS:UniProtKB | P | defense response to virus |
| GO:0045087 | IEA:UniProtKB-KW | P | innate immune response |
| GO:0050709 | ISS:UniProtKB | P | negative regulation of protein secretion |
| GO:0045071 | IEA:Ensembl | P | negative regulation of viral genome replication |
| GO:0001503 | IDA:RGD | P | ossification |
| GO:2000553 | ISS:UniProtKB | P | positive regulation of T-helper 2 cell cytokine production |
| GO:0034157 | ISS:UniProtKB | P | positive regulation of toll-like receptor 7 signaling pathway |
| GO:0034165 | ISS:UniProtKB | P | positive regulation of toll-like receptor 9 signaling pathway |
| GO:0030278 | IDA:RGD | P | regulation of ossification |
| GO:0009615 | ISS:UniProtKB | P | response to virus |
KEGG Pathway Links
Domain Information
UniProt Annotations
Entry Information
Gene Name
radical S-adenosyl methionine domain containing 2
Protein Entry
RSAD2_RAT
UniProt ID
Species
Rat
Comments
| Comment Type | Description |
|---|---|
| Cofactor | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence= ; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. ; |
| Developmental Stage | Not detected in undifferentiated primary osteoblasts. Expression increases during differentiation and declines to much reduced levels in mature osteoblasts |
| Domain | The N-terminal region (1-41) is necessary for its localization to the endoplasmic reticulum membrane and lipid droplet |
| Function | Interferon-inducible iron-sulfur (4FE-4S) cluster- binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of viruses, including west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus and vesicular stomatitis virus (VSV). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphospate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins (By similarity) |
| Induction | By interferon type I, type II and LPS. Interferon alpha induction is rapid and transient, peaks 4-6 hours after stimulation and returns to basal levels 24 hours after stimulation. Interferon gamma elicits a more prolonged response where expression remains elevated 48 hours after stimulation. Induced by infection with Vesicular stomatitis virus and pseudorabies virus, presumably through type I interferon pathway |
| Similarity | Belongs to the radical SAM superfamily. RSAD2 family |
| Subcellular Location | Endoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane protein ; Cytoplasmic side {ECO:0000250}. Lipid droplet . |
| Subunit | Homodimer. Interacts (via C-terminus) with VAPA/VAP33 (via C-terminus) and inhibits its interaction with hepatitis virus C (HCV) protein NS5A. Interacts with HADHB, IRAK1 and TRAF6 (By similarity) |
| Tissue Specificity | In neonatal rat tibia, specifically localized in cells of the periosteum, in osteoblasts lining endosteal and peristeal bone surfaces, to articular surfaces of cartilage and in perichondral cells but not in chondrocytes (at protein level). Expressed predominantly in bone marrow and spleen |
Identical and Related Proteins
Unique RefSeq proteins for LMP012728 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 20302057 | RefSeq | NP_620236 | 360 | radical S-adenosyl methionine domain-containing protein 2 |
Identical Sequences to LMP012728 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:20302057 | EMBL | CAA68971.1 | 360 | Best5 protein [Rattus norvegicus] |
| GI:20302057 | SwissProt | O70600.1 | 360 | RecName: Full=Radical S-adenosyl methionine domain-containing protein 2; AltName: Full=Bone-expressed sequence tag 5 protein; AltName: Full=Viperin; AltName: Full=Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible [Rattus norvegicus] |
Related Sequences to LMP012728 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:20302057 | EMBL | CAA68971.1 | 360 | Best5 protein [Rattus norvegicus] |
| GI:20302057 | GenBank | EDM03204.1 | 362 | rCG62278 [Rattus norvegicus] |
| GI:20302057 | SwissProt | O70600.1 | 360 | RecName: Full=Radical S-adenosyl methionine domain-containing protein 2; AltName: Full=Bone-expressed sequence tag 5 protein; AltName: Full=Viperin; AltName: Full=Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible [Rattus norvegicus] |