Gene/Proteome Database (LMPD)
LMPD ID
LMP012821
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
synaptojanin 1
Gene Symbol
Alternate Names
synaptojanin-1; synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1; synaptic inositol-1,4,5-trisphosphate 5-phosphatase 1;
Chromosome
11
Map Location
11q11
EC Number
3.1.3.36
Summary
has inositol 5-phosphatase activity; interacts with dynamin to play a role in synaptic vesicle recycling [RGD, Feb 2006]
Orthologs
Proteins
| synaptojanin-1 | |
|---|---|
| Refseq ID | NP_445928 |
| Protein GI | 209870039 |
| UniProt ID | Q62910 |
| mRNA ID | NM_053476 |
| Length | 1292 |
| MAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLDLSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSVLKPQVQKFLDYGFFYFDGSAVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQENTFFDDALIDELLQQFAHFGEVILIRFVEDKMWVTFLEGSSALNVLSLNGKELLNRTITITLKSPDWIKTLEEEMSLEKISVTLPSSTSSTLLGEDAEVSADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTAPEYSAPSLPIRPSRAPSRTPGPLSSQGAPVDTQPAAQKESSQTIEPKRPPPPRPVAPPARPAPPQRPPPPSGARSPAPARKEFGAPKSPGTARKDNIGRNQPSPQAGLAGPGPSGYGAARPTIPARAGVISAPQSQARVSAGRLTPESQSKPLETSKGPAVLPEPLKPQAAFPPQPSLPTPAQKLQDPLVPIAAPMPPSIPQSNLETPPLPPPRSRSSQSLPSDSSPQLQQEQPTG | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IDA:RGD | C | cytoplasm |
| GO:0043005 | IDA:RGD | C | neuron projection |
| GO:0043234 | IDA:RGD | C | protein complex |
| GO:0000166 | IEA:InterPro | F | nucleotide binding |
| GO:0004439 | IEA:UniProtKB-EC | F | phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity |
| GO:0034595 | IDA:RGD | F | phosphatidylinositol phosphate 5-phosphatase activity |
| GO:0032403 | IDA:RGD | F | protein complex binding |
| GO:0008022 | IPI:RGD | F | protein C-terminus binding |
| GO:0003723 | IEA:UniProtKB-KW | F | RNA binding |
| GO:0017124 | IDA:RGD | F | SH3 domain binding |
| GO:0007420 | IEP:RGD | P | brain development |
| GO:0006897 | IEA:UniProtKB-KW | P | endocytosis |
| GO:0046856 | IEA:InterPro | P | phosphatidylinositol dephosphorylation |
| GO:0014015 | IMP:RGD | P | positive regulation of gliogenesis |
| GO:0048260 | IMP:RGD | P | positive regulation of receptor-mediated endocytosis |
| GO:0034097 | IMP:RGD | P | response to cytokine |
| GO:0032526 | IMP:RGD | P | response to retinoic acid |
KEGG Pathway Links
Domain Information
InterPro Annotations
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=6; Comment=A stop codon is created in position 1309 of isoform 1 due to alternative splicing. ; Name=1; Synonyms=170 kDa; IsoId=Q62910-1; Sequence=Displayed; Name=2; Synonyms=145 kDa; IsoId=Q62910-2; Sequence=VSP_002686; Name=3; Synonyms=Delta-SAC; IsoId=Q62910-3; Sequence=VSP_002684; Name=4; Synonyms=170 kDa-16AA; IsoId=Q62910-4; Sequence=VSP_002685; Name=5; Synonyms=145 kDa-16AA; IsoId=Q62910-5; Sequence=VSP_002685, VSP_002686; Name=6; Synonyms=Delta-SAC-16AA; IsoId=Q62910-6; Sequence=VSP_002684, VSP_002685; |
| Catalytic Activity | 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate. |
| Developmental Stage | At embryonic day 12 (E12) only isoform 1 is seen while at E16 and E18 isoform 1 and isoform 2 are seen. In the adult brain expression of isoform 2 increases dramatically as compared with its expression in embryonic brain where as isoform 1 decreases to undetectable levels. |
| Domain | Binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats |
| Domain | Splicing of the SAC1 domain does not alter the catalytic activity of synaptojanin 1. |
| Domain | The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2. |
| Function | Inositol 5-phosphatase which has a role in clathrin- mediated endocytosis. |
| Interaction | Q12965:MYO1E (xeno); NbExp=2; IntAct=EBI-1149123, EBI-4279548; Q99962:SH3GL2 (xeno); NbExp=2; IntAct=EBI-1149123, EBI-77938; O35179:Sh3gl2; NbExp=2; IntAct=EBI-1149123, EBI-1149197; O35180:Sh3gl3; NbExp=2; IntAct=EBI-1149123, EBI-1149266; |
| Similarity | Belongs to the synaptojanin family |
| Similarity | Contains 1 RRM (RNA recognition motif) domain |
| Similarity | Contains 1 SAC domain. {ECO:0000255|PROSITE- ProRule:PRU00183}. |
| Similarity | In the central section; belongs to the inositol 1,4,5- trisphosphate 5-phosphatase family |
| Subcellular Location | Cytoplasm . |
| Subunit | Interacts with ASH/GRB2. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Binds AMPH, SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain). {ECO:0000250, ECO:0000269|PubMed:17257598, ECO:0000269|PubMed:9238017}. |
| Tissue Specificity | Isoform 1 is found in neonatal brain, and in a wide variety of adult non-neuronal tissues. Isoform 2 is expressed predominantly in the neurons, but is also found in all other tissues at much lower levels. Isoform 1 and isoform 2 are detected in the lung and heart. Isoform 1 is expressed at higher levels than isoform 2 in the testis and liver and both isoforms are not detected in the skeletal muscle. Isoform 3 with the 16-amino-acid insert is only found in the brain while isoform 3 without the 16- amino-acid insert is found in the lung. |
Identical and Related Proteins
Unique RefSeq proteins for LMP012821 (as displayed in Record Overview)
Identical Sequences to LMP012821 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:209870039 | GenBank | AAB60525.2 | 1292 | 145 kDa synaptojanin isoform [Rattus norvegicus] |
| GI:209870039 | GenBank | EDM10702.1 | 1292 | synaptojanin 1, isoform CRA_c [Rattus norvegicus] |
Related Sequences to LMP012821 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:209870039 | EMBL | CAR81429.1 | 1430 | unnamed protein product [Rattus norvegicus] |
| GI:209870039 | GenBank | AAB60525.2 | 1292 | 145 kDa synaptojanin isoform [Rattus norvegicus] |
| GI:209870039 | GenBank | EDM10702.1 | 1292 | synaptojanin 1, isoform CRA_c [Rattus norvegicus] |