Gene/Proteome Database (LMPD)
LMPD ID
LMP012828
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
protein kinase C, theta
Gene Symbol
Synonyms
Pkcq;
Alternate Names
protein kinase C theta type; nPKC-theta;
Chromosome
17
Map Location
17q12.3
EC Number
2.7.11.13
Summary
mediates the integration of TCR- and CD28-derived signals leading to NFk-B transcription factor activation necessary for efficient IL-2 production and T cell proliferation [RGD, Feb 2006]
Orthologs
Proteins
| protein kinase C theta type | |
|---|---|
| Refseq ID | NP_001263650 |
| Protein GI | 454515948 |
| UniProt ID | F1LM10 |
| mRNA ID | NM_001276721 |
| Length | 707 |
| MSPFLRIGLSNFDCGTCQACQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGRTEIWLELKPQGRMLMNARYFLEMSDTKDMSEFENEGFFALHHRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARTLRDSEHIFREGPIEISFPRSIKSETRPPCVPTPGKKEPQGICWESPLDGADKTAQPPEPEVNLQRASLQLKLKIDDFILHKMLGKGSFGKVFLAEFKRTKQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDRDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEREAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPYDCSNFDKEFLSEKPRLSFADRALINSMDQNMFSNFSFINPGMETLICS | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IEA:UniProtKB-SubCell | C | cytoplasm |
| GO:0001772 | IEA:Ensembl | C | immunological synapse |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0004697 | IEA:UniProtKB-EC | F | protein kinase C activity |
| GO:0006954 | IEA:UniProtKB-KW | P | inflammatory response |
| GO:0035556 | IEA:Ensembl | P | intracellular signal transduction |
| GO:0006509 | IEA:Ensembl | P | membrane protein ectodomain proteolysis |
| GO:0070233 | IEA:Ensembl | P | negative regulation of T cell apoptotic process |
| GO:0046627 | IEA:Ensembl | P | negative regulation of insulin receptor signaling pathway |
| GO:0051092 | IEA:Ensembl | P | positive regulation of NF-kappaB transcription factor activity |
| GO:0042102 | IEA:Ensembl | P | positive regulation of T cell proliferation |
| GO:2000318 | IEA:Ensembl | P | positive regulation of T-helper 17 type immune response |
| GO:2000570 | IEA:Ensembl | P | positive regulation of T-helper 2 cell activation |
| GO:0032740 | IEA:Ensembl | P | positive regulation of interleukin-17 production |
| GO:0045086 | IEA:Ensembl | P | positive regulation of interleukin-2 biosynthetic process |
| GO:0032753 | IEA:Ensembl | P | positive regulation of interleukin-4 production |
| GO:0090330 | IEA:Ensembl | P | regulation of platelet aggregation |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko04920 | Adipocytokine signaling pathway |
| rno04920 | Adipocytokine signaling pathway |
| ko04750 | Inflammatory mediator regulation of TRP channels |
| rno04750 | Inflammatory mediator regulation of TRP channels |
| ko05162 | Measles |
| rno05162 | Measles |
| rno04064 | NF-kappa B signaling pathway |
| ko04660 | T cell receptor signaling pathway |
| rno04660 | T cell receptor signaling pathway |
| ko04530 | Tight junction |
| rno04530 | Tight junction |
| ko04270 | Vascular smooth muscle contraction |
| rno04270 | Vascular smooth muscle contraction |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| 5953415 | Adaptive Immune System |
| 5953314 | Apoptosis |
| 5954021 | Apoptotic cleavage of cellular proteins |
| 5954022 | Apoptotic execution phase |
| 5953532 | Axon guidance |
| 5953533 | Developmental Biology |
| 5953253 | Disease |
| 5953382 | Diseases associated with visual transduction |
| 5954035 | Downstream TCR signaling |
| 5953655 | Effects of PIP2 hydrolysis |
| 5954040 | FCERI mediated NF-kB activation |
| 5953417 | Fc epsilon receptor (FCERI) signaling |
| 5953653 | G alpha (q) signalling events |
| 5954206 | G alpha (z) signalling events |
| 5953642 | GPCR downstream signaling |
| 5953537 | Gastrin-CREB signalling pathway via PKC and MAPK |
| 5953645 | Hemostasis |
| 5953410 | Immune System |
| 5953378 | Inactivation, recovery and regulation of the phototransduction cascade |
| 5953409 | Innate Immune System |
| 5954126 | Netrin-1 signaling |
| 5953644 | Platelet activation, signaling and aggregation |
| 5953381 | Signal Transduction |
| 5953391 | Signaling by GPCR |
| 5954036 | TCR signaling |
| 5953379 | The phototransduction cascade |
| 5953380 | Visual phototransduction |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR000961 | AGC-kinase, C-terminal |
| IPR000008 | C2 domain |
| IPR020454 | Diacylglycerol/phorbol-ester binding |
| IPR014376 | Protein kinase C, delta/epsilon/eta/theta types |
| IPR027264 | Protein kinase C, theta |
| IPR002219 | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain |
| IPR000719 | Protein kinase domain |
| IPR017441 | Protein kinase, ATP binding site |
| IPR017892 | Protein kinase, C-terminal |
| IPR011009 | Protein kinase-like domain |
| IPR008271 | Serine/threonine-protein kinase, active site |
| IPR002290 | Serine/threonine/dual specificity protein kinase, catalytic domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | ATP + a protein = ADP + a phosphoprotein |
| Caution | The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data |
| Cofactor | Note=Magnesium. ; |
| Enzyme Regulation | Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation |
| Function | Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non- redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates to the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non- canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in 'outside-in' signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate PKB/AKT1 |
| Similarity | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily |
| Similarity | Contains AGC-kinase C-terminal domain |
| Similarity | Contains protein kinase domain |
| Subcellular Location | Cytoplasm . Cell membrane ; Peripheral membrane protein . Note=In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse |
| Subunit | Interacts with GLRX3 (via N-terminus). Interacts with ECT2 |
Identical and Related Proteins
Unique RefSeq proteins for LMP012828 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 454515948 | RefSeq | NP_001263650 | 707 | protein kinase C theta type |
Identical Sequences to LMP012828 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|
Related Sequences to LMP012828 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:454515948 | RefSeq | NP_032885.1 | 707 | protein kinase C theta type [Mus musculus] |
| GI:454515948 | SwissProt | Q02111.1 | 707 | RecName: Full=Protein kinase C theta type; AltName: Full=nPKC-theta [Mus musculus] |
| GI:454515948 | SwissProt | Q9WTQ0.2 | 707 | RecName: Full=Protein kinase C theta type; AltName: Full=nPKC-theta [Rattus norvegicus] |