Gene/Proteome Database (LMPD)

LMPD ID
LMP012960
Gene ID
192259
Species
Rattus norvegicus (Rat)
Gene Name
phospholipase B1
Gene Symbol
Plb1
Synonyms
Phlpb;
Alternate Names
phospholipase B1, membrane-associated; PLB/LIP; phospholipase B; phospholipase B/lipase;
Chromosome
6
Map Location
6q13
EC Number
3.1.1.4
Summary
exhibits broad lipolytic activities (PLA2, lysophospholipase, and lipase activities); may mediate terminal digestion, or membrane digestion, of dietary lipids and biliary phospholipids [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Plb1

Proteins

phospholipase B1, membrane-associated precursor
Refseq ID NP_620253
Protein GI 20302091
UniProt ID O54728
mRNA ID NM_138898
Length 1450
MESWPGVSLVGLLLLLLLGQGPSQIHGSSGENTSQPQQVFRTLKNFSFPCKPKKLELSVLSKSVHSLRPSDIKLVAAIGNLETPPAPGSGVVNMEKPQSLESELQNVCIGIMTALSDIIRHFNPSVLMPTCSPGKGTAGHTTIAEDLWIQAKELVRHLKDNPELDFEKDWKLITVLFSNTSQCHLCSSDQQKRHLMKHMEMLSGVLDYLHREVPRAFVNLVDLSEVLTMAQQHQETGFSPAPEICKCSEEITKLSKAVMQWSYQEAWEDLLASSKFNKHETFAVVFQSFFSEVELPLERPSPQDSTTLALRIWNSMMEPVGRKDGTLNEAERKTMKCPSQESPYLFTYRNSNYQARQLKPIGKFQMKEGTKFTCPDKDPSDSIPTTVHRLRPADIKVIGAMGDSLTAGNGAGSSPGNVLDVLTQYRGLSWSVGGDETIETVTTLANILREFNPSLKGFSVGTGKENTPRASFNQAVAGAKSDGLAAQAKKLVSLMKDDKTINFQEDWKIITVFIGGNDLCGSCNNLARFSPQTFTDNIKTALDILHAEVPRAFVNMVSVIEITPLRELFNEPKVSCPRMILRSLCPCVLNLGENSAELAQLVERNRQYQEETGKLIESGRYDTRDDFTVVLQPMFENVVMPRTLEGLPDSSFFAPDCFHFNVKTHARSAIALWKNMLEPVGRKTRHQNFEIKVPIMCPNQTSPFLSTTKNSNLGHGTSMSCEEKAPSASPPTSVHTLRPADIQVVAALGDSVTAGNGISSQEGDLADVTTQYRGLSYSAGGDKFLENVTTLPNILREFNGNLTGYSVGTGDVNSASAFLNQAVPGAKAENLASQVQTLIQKMKNDTRVNFHQDWKVITVMIGASDLCDFCKDSNRYSAANFSDHLRNALDILHKEVPRALVNLVDFMNPSIIRQVFLKNPDKCPVNQTSVLCNCVLTPGEDSHELARLEAFTKSYQSSMLQLVESGRYDTREDFSVVLQPFLFNIRLPILENGNPDTSFFAPDCILLSQKFHTQLARALWANMLEPLGKKMDTLDPKELIALACPTKDKPFLRTFRNSNYTYPIKPAIENWGSDFLCTEQSPSSKVPTSVHELRPSDIKVVAAMGDFLTTATGARPSESSSLDTPWRGLSWSIGGDGTLETHTTLPNILKKFNPSILGFSTGTLENTAGLNVAEEGARAQDMPAQAQALVKKMKSTPTINIQEDWKLITLLIGNNDLCLYCEDPENYSTREYVKYIQHALDIFYEELPRVFINVVEVMELSGLLHDQGGKCAMPLAVQKNCSCLKRSQNLMAMQELKKVNGNLQSALSELSYWHRYMQREDFAVTVQPFFRNTFVPLDERGGLDLTFFSEDCFHFSVRGHAEMAIALWNNMLEPVGKKTTSNNFTYNRTKLKCPSPENPFLYTVRNSQILLDKAKENSNTLYWAVPVAAVGGLVVGILGMMLWRTVRLVQ
sig_peptide: 1..23 inference: non-experimental evidence, no additional details recorded note: Potential; propagated from UniProtKB/Swiss-Prot (O54728.1) calculated_mol_wt: 2380 peptide sequence: MESWPGVSLVGLLLLLLLGQGPS mat_peptide: 24..1450 product: Phospholipase B1, membrane-associated experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (O54728.1) calculated_mol_wt: 158728 peptide sequence: QIHGSSGENTSQPQQVFRTLKNFSFPCKPKKLELSVLSKSVHSLRPSDIKLVAAIGNLETPPAPGSGVVNMEKPQSLESELQNVCIGIMTALSDIIRHFNPSVLMPTCSPGKGTAGHTTIAEDLWIQAKELVRHLKDNPELDFEKDWKLITVLFSNTSQCHLCSSDQQKRHLMKHMEMLSGVLDYLHREVPRAFVNLVDLSEVLTMAQQHQETGFSPAPEICKCSEEITKLSKAVMQWSYQEAWEDLLASSKFNKHETFAVVFQSFFSEVELPLERPSPQDSTTLALRIWNSMMEPVGRKDGTLNEAERKTMKCPSQESPYLFTYRNSNYQARQLKPIGKFQMKEGTKFTCPDKDPSDSIPTTVHRLRPADIKVIGAMGDSLTAGNGAGSSPGNVLDVLTQYRGLSWSVGGDETIETVTTLANILREFNPSLKGFSVGTGKENTPRASFNQAVAGAKSDGLAAQAKKLVSLMKDDKTINFQEDWKIITVFIGGNDLCGSCNNLARFSPQTFTDNIKTALDILHAEVPRAFVNMVSVIEITPLRELFNEPKVSCPRMILRSLCPCVLNLGENSAELAQLVERNRQYQEETGKLIESGRYDTRDDFTVVLQPMFENVVMPRTLEGLPDSSFFAPDCFHFNVKTHARSAIALWKNMLEPVGRKTRHQNFEIKVPIMCPNQTSPFLSTTKNSNLGHGTSMSCEEKAPSASPPTSVHTLRPADIQVVAALGDSVTAGNGISSQEGDLADVTTQYRGLSYSAGGDKFLENVTTLPNILREFNGNLTGYSVGTGDVNSASAFLNQAVPGAKAENLASQVQTLIQKMKNDTRVNFHQDWKVITVMIGASDLCDFCKDSNRYSAANFSDHLRNALDILHKEVPRALVNLVDFMNPSIIRQVFLKNPDKCPVNQTSVLCNCVLTPGEDSHELARLEAFTKSYQSSMLQLVESGRYDTREDFSVVLQPFLFNIRLPILENGNPDTSFFAPDCILLSQKFHTQLARALWANMLEPLGKKMDTLDPKELIALACPTKDKPFLRTFRNSNYTYPIKPAIENWGSDFLCTEQSPSSKVPTSVHELRPSDIKVVAAMGDFLTTATGARPSESSSLDTPWRGLSWSIGGDGTLETHTTLPNILKKFNPSILGFSTGTLENTAGLNVAEEGARAQDMPAQAQALVKKMKSTPTINIQEDWKLITLLIGNNDLCLYCEDPENYSTREYVKYIQHALDIFYEELPRVFINVVEVMELSGLLHDQGGKCAMPLAVQKNCSCLKRSQNLMAMQELKKVNGNLQSALSELSYWHRYMQREDFAVTVQPFFRNTFVPLDERGGLDLTFFSEDCFHFSVRGHAEMAIALWNNMLEPVGKKTTSNNFTYNRTKLKCPSPENPFLYTVRNSQILLDKAKENSNTLYWAVPVAAVGGLVVGILGMMLWRTVRLVQ

Gene Information

Entrez Gene ID
192259
Gene Name
phospholipase B1
Gene Symbol
Plb1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0031526 IDA:RGD C brush border membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0004622 IDA:RGD F lysophospholipase activity
GO:0004623 IDA:RGD F phospholipase A2 activity
GO:0050253 IDA:RGD F retinyl-palmitate esterase activity
GO:0016042 IEA:UniProtKB-KW P lipid catabolic process
GO:0006644 IDA:RGD P phospholipid metabolic process
GO:0042572 IDA:RGD P retinol metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko00590 Arachidonic acid metabolism
rno00590 Arachidonic acid metabolism
ko00565 Ether lipid metabolism
rno00565 Ether lipid metabolism
ko00564 Glycerophospholipid metabolism
rno00564 Glycerophospholipid metabolism
ko00591 Linoleic acid metabolism
rno00591 Linoleic acid metabolism
rno01100 Metabolic pathways
ko04977 Vitamin digestion and absorption
rno04977 Vitamin digestion and absorption
ko00592 alpha-Linolenic acid metabolism
rno00592 alpha-Linolenic acid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR001087 Lipase, GDSL
IPR008265 Lipase, GDSL, active site

UniProt Annotations

Entry Information

Gene Name
phospholipase B1
Protein Entry
PLB1_RAT
UniProt ID
O54728
Species
Rat

Comments

Comment Type Description
Catalytic Activity 2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate.
Catalytic Activity Phosphatidylcholine + H(2)O = 1- acylglycerophosphocholine + a carboxylate.
Domain Repeat 2 contains the catalytic domain.
Enzyme Regulation Inhibited by diisopropyl fluorophosphate.
Function Membrane-associated phospholipase. Exhibits a calcium- independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids. {ECO:0000269|PubMed:11401559, ECO:0000269|PubMed:9442064, ECO:0000269|PubMed:9442065}.
Ptm Undergoes proteolytic cleavage in the ileum
Similarity Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily
Subcellular Location Apical cell membrane ; Single- pass type I membrane protein {ECO:0000269|PubMed:9442064, ECO:0000269|PubMed:9442065}. Note=Present in the intestinal brush border membranes.
Tissue Specificity Expressed in the ileum mucosa, Paneth cells spermatocytes, spermatids and sperm (at protein level). Expressed in the ileum, jejunum, esophagus and testis

Identical and Related Proteins

Unique RefSeq proteins for LMP012960 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
20302091 RefSeq NP_620253 1450 phospholipase B1, membrane-associated precursor

Identical Sequences to LMP012960 proteins

Reference Database Accession Length Protein Name
GI:20302091 DBBJ BAA23813.1 1450 phospholipase B [Rattus norvegicus]
GI:20302091 GenBank AEU43307.1 1450 Sequence 56 from patent US 8052970
GI:20302091 SwissProt O54728.1 1450 RecName: Full=Phospholipase B1, membrane-associated; Short=Phospholipase B; AltName: Full=Phospholipase B/lipase; Short=PLB/LIP; Includes: RecName: Full=Phospholipase A2; Includes: RecName: Full=Lysophospholipase; Flags: Precursor [Rattus norvegicus]

Related Sequences to LMP012960 proteins

Reference Database Accession Length Protein Name
GI:20302091 DBBJ BAA23813.1 1450 phospholipase B [Rattus norvegicus]
GI:20302091 GenBank EDM02885.1 1423 phospholipase B, isoform CRA_c [Rattus norvegicus]
GI:20302091 GenBank AEU43307.1 1450 Sequence 56 from patent US 8052970
GI:20302091 SwissProt O54728.1 1450 RecName: Full=Phospholipase B1, membrane-associated; Short=Phospholipase B; AltName: Full=Phospholipase B/lipase; Short=PLB/LIP; Includes: RecName: Full=Phospholipase A2; Includes: RecName: Full=Lysophospholipase; Flags: Precursor [Rattus norvegicus]