Lipidomics Methods and Protocols
Analytical methods were selected from special issues and review papers published by LIPID MAPS® Consortium members, maintaining some of the traditional and introducing new ones with the latest technology and instrumentation. These protocols are specific to each lipid category but can be also applied to several types of biological matrices.
Methods organized by lipid category
Fatty acyls
- Fatty acids
- High Sensitivity Quantitative Lipidomics Analysis of Fatty Acids in Biological Samples by GC-MS (Sample source: mammalian cells and tissue)
- LIPID MAPS® Fatty Acid GC-MS Protocol (Sample source: mammalian cells, media, plasma or tissue)
- 3-hydroxy fatty acid analysis by GC-MS (Sample source: human blood plasma)
- LC-MS Detection of Omega-Oxidation Products of Aliphatic Fatty Acids in Exhaled Breath (Sample source: human breath)
- Oxidized/nitrated fatty acid analysis by GC-MS/MS and LC-MS/MS (Sample source: blood and mammalian tissue)
- LC-MS/MS Quantification of derivatised Short chain fatty acids (Sample source: human feces and murine colon content)
- Eicosanoids
- LC-MS analysis of fatty acid derived eicosanoids and N-acylethanolamines (Sample source: mammalian tissue/cultured cells)
- Detection and Quantitation of Eicosanoids via High Performance LC-MS (Sample source: mammalian tissue/cultured cells)
- Eicosanoid LC-MS Protocol (Sample source: mammalian cell media)
- Quantification of fatty acid oxidation products using LC-MS (Sample source: human urine and plasma)
- Cellular Lipid Extraction for Targeted Stable Isotope Dilution LC-MS analysis (Sample source: mammalian cell lysate)
- UPLC-MS analysis of eicosanoids (Sample source: human plasma and tissue)
Glycerolipids
- Mono/di/triacylglycerols, Monoether diacylglycerols
- LC-MS Analysis of Neutral Glycerolipids (Sample source: mammalian cultured (RAW) cells)
- LC-MS Analysis of Neutral lipids from human plasma (Sample source: human plasma)
- LC-MS Analysis of Neutral lipids from cells (Sample source: mammalian cells)
Glycerophospholipids
- PA,PC,PE,PS,PG,PI
- Glycerophospholipid Identification and Quantitation by LC-MS (Sample source: mammalian cultured (RAW) cells)
- LC-MS analysis of esterified oxylipins in phospholipids (Sample source: mammalian tissue (lung, liver))
- An accurate and versatile method for determining the acyl group- introducing position of lysophospholipid acyltransferases (Sample source: human HEK293A cells (primary embryonal human kidney))
Sphingolipids
- Multiple sphingolipid classes
- Analysis of mammalian sphingolipids by LC-MS/MS and MALDI (Sample source: mammalian cultured (RAW) cells)
- Structure-Specific, Quantitative Methods for Analysis of Sphingolipids by LC-MS/MS (Sample source: mammalian blood)
Sterol lipids
- Sterols
- Extraction and Analysis of Sterols in Biological Matrices by LC-MS (Sample source: mammalian cells and tissues)
- Sterol Extraction and Quantitation by LC-MS (Sample source: mammalian cultured cells)
- Sterol Extraction, Cleanup, and Quantitation by LC-MS (Sample source: mammalian macrophages)
- Quantitative analysis of 4-hydroxycholesterol in human plasma and serum by UHPLC/ESI-HR-MS (Sample source: human plasma/serum)
- Cholesterol esters
- LC-MS Analysis of Neutral lipids from human plasma (Sample source: human plasma)
- LC-MS Analysis of Neutral lipids from cells (Sample source: mammalian cells)
Prenol lipids
- Ubiquinones, Dolichols
- Analysis of Ubiquinones, Dolichols, and Dolichol Diphosphate-Oligosaccharides by LC-MS (Sample source: mammalian cultured (RAW) cells)
- Human plasma neutral folch extraction and LC-MS analysis (Sample source: human plasma)
Multiple lipid categories
- Glycerophospholipids, Sphingolipids
- 3-D enhanced lipidomics analysis using differential ion mobility MS (Sample source: human serum)