Protocol

Protocol IDs are assigned by LIPID MAPS® Laboratory Information Management (LIMS) software1. The format of the IDs is as follows:

  • Procedure protocols begin with the letters "PP." Solution protocols begin with the letters "PS."
  • The last 2 numbers of a protocol ID indicate the version number. "00" indicates the initial version. Subsequent revisions of the protocol possess IDs that end with "01", "02", and so on.
  • The version with the largest set of ending numbers is the currently accepted protocol. Previous versions are considered obsolete.

Any core can write a protocol. Except for core D, the title of the new protocol should include the core. Core D has responsibility for composing consortium-wide protocols involving preparation, handling, and treatment of cells and tissues. Other cores can write protocols that provide additional detail in these areas for their own guidance, provided:

  • The protocols do not contradict or conflict with Core D protocols.
  • The protocols reference pertinent Core D protocols.
  • The protocol title includes the core.

See Also

Please see also our Lipidomics Methods tutorials.

References

1. A Laboratory information management system for high throughput experimental lipidomics: Minimal information required for the analysis of lipidomics experiments (MIALE). Byrnes R, Fahy E and Subramaniam S. Journal of the Association for Laboratory Automation 12, 230-238 (2007) [doi:10.1016/j.jala.2007.04.002]

Equipment and supplies

Recommended Equipment Equipment
Tissue Culture Supplies Supplies
RAW 264.7 Tissue Culture Supplies PP0000001600 Added 08/27/07
RAW 264.7 Cells Recommended Equipment PP0000001700 Added 08/27/07

Procedure Protocols

General

Thawing and Passage Procedure PP0000000101
Thawing and passage procedure for RAW 264.7 cells PP0000000100 Added 08/27/07
Use of Animals PP0000001100 Added 08/27/07
Ordering Mice PP0000001200 Added 08/27/07
Ordering Mice for Foam Cells PP0000002900 Added 08/22/06
LIPID MAPS DNA Assay PP0000002700
Fatty Acid Mass Spectrometry Protocol PP0000003800 Added 10/09/12
Fatty Acid Mass Spectrometry Protocol PP0000005300 Added 10/09/12
Fatty Acid Mass Spectrometry Protocol PP0000005301 Added 10/09/12
Harvesting and Plating Thioglycollate Elicited Macrophages for LM Distribution PP0000004101 Added 05/21/08
Core D - Procedure Protocol ATP, Kdo2-Lipid A Treatment PP0000004601 Added 01/04/10
Core D - Procedure Protocol ATP, Kdo2-Lipid A Treatment PP0000004602 Added 01/12/10
Procedure Protocol for Harvesting and Plating Bone Marrow Derived Macrophages for LIPID MAPS Distribution PP0000004701 Added 01/04/10
Procedure Protocol for Harvesting and Plating Bone Marrow Derived Macrophages for LIPID MAPS Distribution PP0000004702 Added 01/12/10
Protocol ATP/POVPC-peptide Costimulation Time course PP0000005101 Added 10/09/12
Protocol ATP/PAPC/oxidized PAPC Costimulation Time course PP0000005200 Added 10/09/12

Kdo2-Lipid A Treatment

LPS Induction (Serum-deprived) PP0000000600
10% Serum Kdo2-Lipid A Treatment PP0000001000 Added 08/27/07
10% Serum Kdo2-Lipid A Treatment PP0000001001
10% Serum Kdo2-Lipid A Treatment PP0000001002
10% Serum Kdo2-Lipid A Treatment PP0000001003
10% Serum Kdo2-Lipid A Treatment PP0000001004
0.5% Serum Kdo2-Lipid A Treatment PP0000000500 Added 08/27/07
0.5% Serum Kdo2-Lipid A Treatment PP0000000501 Added 08/27/07

Primary Macrophages

Eliciting Bio-Gel Derived Macrophages PP0000001300 Added 08/27/07
Eliciting Foam Cells PP0000003000 Added 08/22/06
Eliciting Thioglycollate Derived Macrophages PP0000001400 Added 08/27/07
Eliciting Thioglycollate derived Macrophages PP0000001401 Added 06/20/07
Harvesting and Plating Bone Marrow-derived Macrophages PP0000003500 Added 06/20/07
Harvesting and Plating Primary Macrophages PP0000001501
Harvesting and Plating Thioglycollate Elicited Macrophages PP0000001502 Added 06/20/07
Harvesting Foam Cells PP0000003100 Added 08/22/06
Harvesting Macrophages PP0000001500 Added 08/27/07
Kdo2-Lipid A Treatment of Thioglycollate and Bone Marrow Derived Macrophages PP0000001801 Added 06/20/07
Primary Macrophage Treatment PP0000001800

Core D - Macrophage Biology and Functional Genomics

Core D - Procedure Protocol Compactin, Kdo2 Lipid A Treatment 2008 PP0000004000 Added 10/09/12
Core D - 25OHC Treatment PP0000004900 Added 11/24/10

Core E - Glycerolipids/Cholesteryl Esters

Core E - LM Thawing and Passage for RAW 264.7 PP0000001900 Added 08/27/07
Core E - 0.5% Serum Kdo2-Lipid A Treatment PP0000002000 Added 08/27/07
Core E - 10% Serum Kdo2-Lipid A Treatment PP0000002100 Added 08/27/07
Core E - Analysis of Neutral Lipids PP0000002200 Added 08/27/07
Core E - Analysis of Neutral Lipids from Human Plasma June 4, 2010 PP0000005000 Added 10/09/12
Core E - Analysis of Neutral Lipids from Cells June 4, 2010 PP0000005500 Added 10/09/12

Core G - Eicosanoids

Core G - Eicosanoid MS PP0000000200 Added 08/27/07
Core G - Eicosanoid MS PP0000000201 Added 08/27/07
Core G - Eicosanoid MS PP0000000204 Added 08/27/07
Core G - Eicosanoid MS PP0000000205 Added 08/27/07
Core G - Eicosanoid MS PP0000000206 Added 08/27/07
Core G - RAW subcellular fractionation PP0000004300 Added 05/19/08
Core G - RAW subcellular fractionation PP0000004301 Added 06/10/08

Core H - Glycerophospholipids

Core H - Direct Injection MS PP0000000900 Added 08/27/07
Core H - HPLC phospholipid protocol PP0000000800 Added 08/27/07
Phospholipid LC-MS Parameters PP0000002600 Added 08/27/07
Core H - Glycerophospholipid Extraction PP0000004200 Added 04/23/08

Core J - Sterols

Core J - 10% NCLPPS, Compactin, Kdo2-Lipid A Treatment PP0000002300 Added 08/27/07
Core J - Compactin Kdo2-Lipid A Treatment in Dye-Free GMed PP0000002400 Added 08/27/07
Core J - Sterol Extraction and LCMS PP0000002500 Added 08/27/07
Core J - Compactin Kdo2-Lipid A Treatment PP0000002800 Added 08/27/07
Core J - Compactin Kdo2-Lipid A Modified PP0000003300 Added 08/27/07
Core J - Compactin Kdo2lipidA RNA PP0000003400 Added 08/27/07
Core J - Sterol Extraction and Quantitation PP0000003200 Added 08/27/07
Core J - Sterol Extraction and Quantitation PP0000003201 Added 08/27/07
Core J - Foam Cell Sterol Extraction and Quantitation PP0000003600 Added 05/21/07
Core J - Sterol Extraction, Cleanup, and Quantitation PP0000003700 Added 05/21/07
Core J - Procedure Protocol - Compactin, Kdo2 Lipid A Treatment 2008 PP0000003900 Added 11/22/10
Core J - Procedure Protocol Compactin, Kdo2 Lipid A Treatment in Dye-Free Growth Medium 2008 PP0000004400 Added 10/09/12
Core J - Human Plasma Samples First Set of Practice and NIST Plasmas PP0000004500 Added 10/09/12

Core K - Prenols and Other Lipids

Core K - Human plasma SRM neutral folch extraction and lipid analysis PP0000004800 Added 10/09/12