LMPD Database

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LMP006853

UniProt Annotations

Entry Information
Gene Name1-phosphatidylinositol-3-phosphate 5-kinase
Protein EntryFAB1_YEAST
UniProt IDP34756
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityATP + 1-phosphatidyl-1D-myo-inositol 3- phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
Catalytic ActivityATP + 1-phosphatidyl-1D-myo-inositol 3- phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate. {ECO:0000269|PubMed:9811604}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
Enzyme RegulationActivated by VAC14 and VAC7. VAC14 acts as a specific osmotic response regulator. {ECO:0000269|PubMed:11889142, ECO:0000269|PubMed:11950935}.
FunctionThe PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. The product of the reaction it catalyzes functions as an important regulator of vacuole homeostasis perhaps by controlling membrane flux to and/or from the vacuole. Hyperosmotic shock-induced increase in the levels of PtdIns(3,5)P2 requires the presence of VAC7, VAC14, and/or FIG4. {ECO:0000269|PubMed:16492811, ECO:0000269|PubMed:9811604}.
InteractionP42837:FIG4; NbExp=4; IntAct=EBI-6754, EBI-28407; Q06708:VAC14; NbExp=9; IntAct=EBI-6754, EBI-27189;
MiscellaneousPresent with 149 molecules/cell in log phase SD medium
MiscellaneousPresent with 149 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
SimilarityContains 1 FYVE-type zinc finger
SimilarityContains 1 FYVE-type zinc finger. {ECO:0000255|PROSITE-ProRule:PRU00091}.
SimilarityContains 1 PIPK domain. {ECO:0000255|PROSITE- ProRule:PRU00781}.
Subcellular LocationVacuole membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note=Localization to the vacuole membrane requires VAC14 and FIG4.
SubunitComponent of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold
SubunitComponent of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold. {ECO:0000269|PubMed:18653468, ECO:0000269|PubMed:19037259}.