LMPD Database

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LMP006858

UniProt Annotations

Entry Information
Gene Namephenylpyruvate decarboxylase ARO10
Protein EntryARO10_YEAST
UniProt IDQ06408
SpeciesYeast (S288c)
Comments
Comment typeDescription
BiotechnologyFusel oils are important flavor and aroma compounds in yeast-fermented products contributing to the quality of beverages and food. In low concentration they are generally desirable, whereas high concentrations may spoil the product. By adjusting growth conditions and substrate their production is sought to be influenced. Phenylethanol, having a rose-like aroma, is an important fragrance in the cosmetic industry and can be produced by fermentation.
Catalytic Activity3-(indol-3-yl)pyruvate = 2-(indol-3- yl)acetaldehyde + CO(2).
Catalytic ActivityA 2-oxo acid = an aldehyde + CO(2).
Catalytic ActivityPhenylpyruvate = phenylacetaldehyde + CO(2).
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ; Note=Binds 1 Mg(2+) per subunit. ;
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Binds 1 Mg(2+) per subunit. {ECO:0000250};
CofactorName=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000250}; Note=Binds 1 thiamine pyrophosphate per subunit. {ECO:0000250};
CofactorName=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence= ; Note=Binds 1 thiamine pyrophosphate per subunit. ;
FunctionOne of five 2-oxo acid decarboxylases (PDC1, PDC5, PDC6, ARO10, and THI3) involved in amino acid catabolism. The enzyme catalyzes the decarboxylation of amino acids, which, in a first step, have been transaminated to the corresponding 2-oxo acids (alpha-keto-acids). In a third step, the resulting aldehydes are reduced to alcohols, collectively referred to as fusel oils or alcohols. Its preferred substrates are the transaminated amino acids, phenylalanine, tryptophan, (and probably tyrosine), but also isoleucine, whereas leucine is a low efficiency and valine and pyruvate are no substrates. In analogy to the pyruvate decarboxylases the enzyme may in a side-reaction catalyze condensation (or carboligation) reactions leading to the formation of 2-hydroxy ketone, collectively called acyloins. {ECO:0000269|PubMed:10207060, ECO:0000269|PubMed:10753893, ECO:0000269|PubMed:12499363}.
InductionExpression is induced by the presence of aromatic amino acids and the lack of preferred nitrogen sources (e.g. ammonia, glutamine) and requires the transcription activator ARO80.
MiscellaneousPresent with 6560 molecules/cell in log phase SD medium
MiscellaneousPresent with 6560 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayAmino-acid degradation; Ehrlich pathway.
SimilarityBelongs to the TPP enzyme family
SimilarityBelongs to the TPP enzyme family. {ECO:0000305}.
Subcellular LocationCytoplasm .
Subcellular LocationCytoplasm {ECO:0000269|PubMed:14562095}.