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LMPD Database

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LMP007122

UniProt Annotations

Entry Information

Gene Name	phosphatidylinositol 3-kinase VPS34
Protein Entry	VPS34_YEAST
UniProt ID	P22543
Species	Yeast (S288c)

Comments

Comment type	Description
Catalytic Activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Enzyme Regulation	Phosphatidylinositol 3-kinase activity is directly dependent on VPS15 protein kinase activity.
Function	Phosphatidylinositol 3-kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy- specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3- kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Its activation by VPS15 may lead to the phosphorylation of phosphatidylinositol in the sorting compartment membrane. Finally, it might also be involved in ethanol tolerance and cell wall integrity. {ECO:0000269\|PubMed:11157979, ECO:0000269\|PubMed:11523784, ECO:0000269\|PubMed:12244127, ECO:0000269\|PubMed:2247081, ECO:0000269\|PubMed:2555343, ECO:0000269\|PubMed:3062374, ECO:0000269\|PubMed:7929582, ECO:0000269\|PubMed:7989323, ECO:0000269\|PubMed:8385367, ECO:0000269\|PubMed:8387919}.
Interaction	P08539:GPA1; NbExp=3; IntAct=EBI-20405, EBI-7376;
Miscellaneous	Present with 1080 molecules/cell in log phase SD medium
Miscellaneous	Present with 1080 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Ptm	Autophosphorylated. Might also be phosphorylated by VPS15.
Similarity	Belongs to the PI3/PI4-kinase family
Similarity	Belongs to the PI3/PI4-kinase family. {ECO:0000255\|PROSITE-ProRule:PRU00880}.
Similarity	Contains 1 C2 PI3K-type domain. {ECO:0000255\|PROSITE- ProRule:PRU00880}.
Similarity	Contains 1 PI3K/PI4K domain. {ECO:0000255\|PROSITE- ProRule:PRU00269}.
Similarity	Contains 1 PIK helical domain. {ECO:0000255\|PROSITE- ProRule:PRU00878}.
Subcellular Location	Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.
Subunit	Component of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34, ATG14, AND ATG38; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38. Interacts directly with ATG38. {ECO:0000269\|PubMed:11157979, ECO:0000269\|PubMed:24165940, ECO:0000269\|PubMed:8387919}.