LMPD Database

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LMP007328

UniProt Annotations

Entry Information
Gene NameArc1p
Protein EntryARC1_YEAST
UniProt IDP46672
SpeciesYeast (S288c)
Comments
Comment typeDescription
FunctionBinds to tRNA and functions as a cofactor for the methionyl-tRNA synthetase (MetRS) and glutamyl-tRNA synthetase (GluRS). Forms a complex with MetRS and GluRS and increases their affinity for cognate tRNAs due to the presence of a tRNA binding domain in its middle and C-terminal part. Binds specifically G4 quadruplex nucleic acid structures (these are four-stranded right- handed helices, stabilized by guanine base quartets). Also required for cytoplasmic confinement of the synthetases and tRNA. {ECO:0000269|PubMed:11069915, ECO:0000269|PubMed:17131041, ECO:0000269|PubMed:8895587, ECO:0000269|PubMed:9659920}.
InteractionP46655:GUS1; NbExp=7; IntAct=EBI-7224, EBI-18665; P00958:MES1; NbExp=7; IntAct=EBI-7224, EBI-18762;
MiscellaneousPresent with 57700 molecules/cell in log phase SD medium
MiscellaneousPresent with 57700 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
SimilarityBelongs to the tRNA-aminoacylation cofactor ARC1 family
SimilarityBelongs to the tRNA-aminoacylation cofactor ARC1 family. {ECO:0000305}.
SimilarityContains 1 tRNA-binding domain. {ECO:0000255|PROSITE- ProRule:PRU00209}.
Subcellular LocationCytoplasm {ECO:0000269|PubMed:11726524, ECO:0000269|PubMed:15710377, ECO:0000269|PubMed:17131041, ECO:0000269|PubMed:8895587}. Note=Largely excluded from the nucleus.
SubunitComponent of a yeast aminoacyl-tRNA synthase (aaRS) complex formed by methionyl-tRNA synthase MES1, glutamyl-tRNA synthase GUS1 and the tRNA aminoacylation cofactor ARC1 in a stoichiometric complex. Interacts (via N-ter) with MES1 (via N- ter) and GUS1 (via N-ter). Can also form a stable binary complex with either MES1 or GUS1 that is functional in terms of aminoacylation. {ECO:0000269|PubMed:11069915, ECO:0000269|PubMed:11726524, ECO:0000269|PubMed:17139087, ECO:0000269|PubMed:8895587, ECO:0000269|PubMed:9659920}.