LMPD Database

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LMP007535

UniProt Annotations

Entry Information

Gene Name	adenine deaminase
Protein Entry	ADE_YEAST
UniProt ID	P53909
Species	Yeast (S288c)

Comments

Comment type	Description
Biophysicochemical Properties	Kinetic parameters: KM=55 uM for adenine {ECO:0000269\|PubMed:18673302}; pH dependence: Optimum pH is 7.0. {ECO:0000269\|PubMed:18673302}; Temperature dependence: Optimum temperature is 30-37 degrees Celsius. {ECO:0000269\|PubMed:18673302};
Catalytic Activity	Adenine + H(2)O = hypoxanthine + NH(3). {ECO:0000255\|HAMAP-Rule:MF_03145}.
Cofactor	Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000255\|HAMAP-Rule:MF_03145}; Note=Binds 1 zinc ion per subunit. {ECO:0000255\|HAMAP- Rule:MF_03145};
Function	Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism. Also exhibits a low activity towards N(6)-substituted adenines that are commonly known as the plant hormones cytokinins. {ECO:0000255\|HAMAP-Rule:MF_03145, ECO:0000269\|PubMed:14643670, ECO:0000269\|PubMed:1577682, ECO:0000269\|PubMed:17517885, ECO:0000269\|PubMed:18673302}.
Induction	Reduced when grown in a poor nitrogen source medium and strongly down-regulated when cells enter quiescence under nutrient-limiting conditions. {ECO:0000269\|PubMed:1577682}.
Miscellaneous	Present with 20700 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Ptm	Probably ubiquitinated when cells enter quiescence in response to nutrient limitation, since it is specifically degraded via a process requiring the F-box protein SAF1 and components of the SKP1-Cullin-F-box complex. {ECO:0000269\|PubMed:17517885}.
Similarity	Belongs to the adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily. {ECO:0000255\|HAMAP- Rule:MF_03145}.
Subcellular Location	Cytoplasm {ECO:0000255\|HAMAP-Rule:MF_03145, ECO:0000269\|PubMed:14562095}. Nucleus {ECO:0000255\|HAMAP- Rule:MF_03145, ECO:0000269\|PubMed:14562095}.