LMPD Database

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LMP010162

UniProt Annotations

Entry Information
Gene NameDol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
Protein EntryALG3_ARATH
UniProt IDO82244
SpeciesArabidopsis
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Name=1; IsoId=O82244-1; Sequence=Displayed; Name=2; IsoId=O82244-2; Sequence=VSP_041719; Name=3; IsoId=O82244-3; Sequence=VSP_041720;
Catalytic ActivityDolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc- diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man- alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta- (1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. {ECO:0000269|PubMed:18567790}.
Disruption PhenotypeNo obvious phenotype under normal and high temperature or salt/osmotic stress conditions. {ECO:0000269|PubMed:20356820}.
FunctionRequired for N-linked oligosaccharide assembly. Adds the sixth mannose residue in an alpha-1,3 linkage onto the dolichol- PP-oligosaccharide precursor dolichol-PP-Man(5)GlcNAc(2). {ECO:0000269|PubMed:18567790, ECO:0000269|PubMed:20356820}.
MiscellaneousIn the absence of ALG3 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant.
PathwayProtein modification; protein glycosylation.
SimilarityBelongs to the glycosyltransferase 58 family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000269|PubMed:18567790, ECO:0000269|PubMed:20356820}; Multi- pass membrane protein {ECO:0000269|PubMed:18567790, ECO:0000269|PubMed:20356820}.