LMPD Database

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LMP010163

UniProt Annotations

Entry Information
Gene NameDol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Protein EntryALG9_ARATH
UniProt IDQ9FZ49
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic ActivityDolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha- (1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc- diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man- alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)- D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. {ECO:0000269|PubMed:20023196}.
Catalytic ActivityDolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)- D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha- (1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)- D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D- GlcNAc-diphosphodolichol + dolichyl phosphate. {ECO:0000269|PubMed:20023196}.
FunctionRequired for N-linked oligosaccharide assembly. Adds the seventh and the ninth mannose residues in an alpha-1,2 linkage onto the dolichol-PP-oligosaccharide precursors dolichol-PP- Man(6)GlcNAc(2) and dolichol-PP-Man(8)GlcNAc(2). {ECO:0000269|PubMed:20023196}.
MiscellaneousIn the absence of ALG9 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant.
PathwayProtein modification; protein glycosylation.
SimilarityBelongs to the glycosyltransferase 22 family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.