LMPD Database

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LMP010164

UniProt Annotations

Entry Information
Gene NameDol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Protein EntryALG12_ARATH
UniProt IDA8MR93
SpeciesArabidopsis
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=6; Name=1; IsoId=A8MR93-1; Sequence=Displayed; Name=2; IsoId=A8MR93-2; Sequence=VSP_041716, VSP_041718; Name=3; IsoId=A8MR93-3; Sequence=VSP_041715; Name=4; IsoId=A8MR93-4; Sequence=VSP_041716, VSP_041717; Name=5; IsoId=A8MR93-5; Sequence=VSP_041717; Name=6; IsoId=A8MR93-6; Sequence=VSP_041716;
Catalytic ActivityDolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D- GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha- (1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)- D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha-(1->6))-D-Man- beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. {ECO:0000269|PubMed:20023196}.
FunctionRequired for N-linked oligosaccharide assembly. Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol- PP-oligosaccharide precursor dolichol-PP-Man(7)GlcNAc(2). {ECO:0000269|PubMed:20023196}.
MiscellaneousIn the absence of ALG12 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant.
PathwayProtein modification; protein glycosylation.
SimilarityBelongs to the glycosyltransferase 22 family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.