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LMP011076

UniProt Annotations

Entry Information

Gene Name	protein abscisic acid-insensitive 5
Protein Entry	ABI5_ARATH
UniProt ID	Q9SJN0
Species	Arabidopsis

Comments

Comment type	Description
Developmental Stage	Expressed in embryo during the latest stages of seed maturation. {ECO:0000269\|PubMed:12084834}.
Disruption Phenotype	Exhibits abscisic acid (ABA) insensitivity. {ECO:0000269\|PubMed:10929936}.
Function	Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence. {ECO:0000269\|PubMed:11287670, ECO:0000269\|PubMed:12000684, ECO:0000269\|PubMed:12084834, ECO:0000269\|PubMed:12177466, ECO:0000269\|PubMed:12410810, ECO:0000269\|PubMed:12434021, ECO:0000269\|PubMed:15118859, ECO:0000269\|PubMed:16247556, ECO:0000269\|PubMed:16463099}.
Induction	Up-regulated by drought, salt, abscisic acid (ABA) and glucose or 2-deoxy-glucose (2DG). Autoregulated. Positively regulated by the light-signaling component HY5. {ECO:0000269\|PubMed:11287670, ECO:0000269\|PubMed:12177466, ECO:0000269\|PubMed:12376636, ECO:0000269\|PubMed:12970489, ECO:0000269\|PubMed:16463099, ECO:0000269\|PubMed:18332440}.
Interaction	Q9FY48:KEG; NbExp=2; IntAct=EBI-1778690, EBI-1955729;
Ptm	Phosphorylated by SRK2D and SRK2I in vitro. {ECO:0000269\|PubMed:11287670, ECO:0000269\|PubMed:12410810, ECO:0000269\|PubMed:12434021, ECO:0000269\|PubMed:17307925}.
Ptm	Ubiquitinated. AFP1, KEG and RPN10 mediate its proteasome- dependent degradation. Its stability or degradation plays a central role in abscisic acid response. Sumoylated at Lys-391 by SIZ1. Sumoylation protects ABI5 from proteasome degradation, attenuating ABA signaling and sensitivity to ABA. {ECO:0000269\|PubMed:19276109}.
Similarity	Belongs to the bZIP family. ABI5 subfamily. {ECO:0000305}.
Similarity	Contains 1 bZIP (basic-leucine zipper) domain. {ECO:0000255\|PROSITE-ProRule:PRU00978}.
Subcellular Location	Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00978, ECO:0000269\|PubMed:12410810}.
Subunit	DNA-binding homodimer. DNA-binding heterodimer with AREB3/DPBF3 or EEL/DPBF4. Interacts with ABI3, KEG, the mediator subunit MED25, and the AFP proteins AFP1, AFP2, AFP3 and AFP4. {ECO:0000269\|PubMed:11489176, ECO:0000269\|PubMed:12569131, ECO:0000269\|PubMed:16247556, ECO:0000269\|PubMed:17194765, ECO:0000269\|PubMed:18484180, ECO:0000269\|PubMed:22822206}.
Tissue Specificity	Predominantly expressed in seeds. {ECO:0000269\|PubMed:10760247, ECO:0000269\|PubMed:12376636}.