Lipid Standards: Eicosanoids
LC Retention Times
LC retention time and MS/MS data were obtained using the conditions outlined in the protocols belowEicos LC Protocol[1]| Eicos LC Protocol_V2[2]| MS/MS Protocol
| lm_id | name | Systematic Name | MS/MS | R.T.(min)[1] | R.T.(min)[2] |
|---|---|---|---|---|---|
| LMFA03010139 | tetranor-PGFM | 9S,11R-dihydroxy-15-oxo-2,3,4,5-tetranor-prostan-1,20-dioic acid | 329([M-H]-) | 2.55 | 2.35 |
| LMFA03010032 | tetranor-PGEM | 11R-hydroxy-9,15-dioxo-2,3,4,5-tetranor-prostan-1,20-dioic acid | 327([M-H]-) | 2.8 | 2.45 |
| LMFA03010029 | 20-hydroxy-PGF2a | 9S,11S,15S,20-tetrahydroxy-5Z,13E-prostadienoic acid | 369([M-H]-) | 2.91 | 2.8 |
| LMFA03010014 | 20-hydroxy-PGE2 | 9-oxo-11R,15S,20-trihydroxy-5Z,13E-prostadienoic acid | 367([M-H]-) | 2.94 | 2.91 |
| LMFA03010024 | 19R-hydroxy-PGE2 | 9-oxo-11R,15S,19R-trihydroxy-5Z,13E-prostadienoic acid | 367([M-H]-) | 2.95 | 2.97 |
| LMFA03010001 | 6-keto-PGF1α | 6-oxo-9S,11R,15S-trihydroxy-13E-prostenoic acid | 369([M-H]-) | 3.4 | 4.71 |
| LMFA03010012 | 6keto-PGE1 | 6,9-dioxo-11R,15S-dihydroxy-13E-prostenoic acid | 367([M-H]-) | 3.66 | 5.19 |
| LMFA03010025 | PGF2β | 9R,11R,15S-trihydroxy-5Z,13E-prostadienoic acid | 353([M-H]-) | 4.09 | 6.59 |
| LMFA03030002 | TXB2 | 9S,11,15S-trihydroxy-thromboxa-5Z,13E-dien-1-oic acid | 369([M-H]-) | 4.2 | 6.61 |
| LMFA03010013 | 6,15-diketo-13,14-dihydro-PGF1α | 6,15-dioxo-9S,11R-dihydroxyprostanoic acid | 369([M-H]-) | 4.24 | 6.13 |
| LMFA03010036 | 11β-PGF2α | 9S,11S,15S-trihydroxy-5Z,13E-prostadienoic acid | 353([M-H]-) | 4.34 | 6.58 |
| LMFA03010002 | PGF2α | 9S,11R,15S-trihydroxy-5Z,13E-prostadienoic acid | 353([M-H]-) | 4.72 | 7.35 |
| LMFA03010011 | 2,3-dinor 11β-PGF2a | 9S,11S,15S-trihydroxy-2,3-dinor-5Z,13E-prostadienoic acid | 325([M-H]-) | 3.48 | 4.86 |
| LMFA03010003 | PGE2 | 9-oxo-11R,15S-dihydroxy-5Z,13E-prostadienoic acid | 351([M-H]-) | 5.19 | 7.54 |
| LMFA03010023 | PGK2 | 9,11-dioxo-15S-hydroxy-5Z,13E-prostadienoic acid | 349([M-H]-) | 5.42 | 8.3 |
| LMFA03010026 | 15-keto-PGF2α | 9S,11R-dihydroxy-15-oxo-5Z,13E-prostadienoic acid | 351([M-H]-) | 5.47 | 7.89 |
| LMFA03010004 | PGD2 | 9S,15S-dihydroxy-11-oxo-5Z,13E-prostadienoic acid | 351([M-H]-) | 5.69 | 7.89 |
| LMFA03010030 | 15-keto-PGE2 | 9,15-dioxo-11R-hydroxy-5Z,13E-prostadienoic acid | 349([M-H]-) | 6.03 | 8.02 |
| LMFA03010027 | 13,14-dihydro-15-keto-PGF2α | 9S,11S-dihydroxy-15-oxo-5Z-prostenoic acid | 353([M-H]-) | 6.57 | 8.76 |
| LMFA03040001 | 5S,6R-LipoxinA4 | 5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid | 351([M-H]-) | 6.78 | 8.58 |
| LMFA03010031 | 13,14-dihydro-15-keto-PGE2 | 9,15-dioxo-11R-hydroxy-5Z-prostenoic acid | 351([M-H]-) | 6.81 | 8.6 |
| LMFA03010010 | PGH2 | 9S,11R-epidioxy-15S-hydroxy-5Z,13E-prostadienoic acid | 351([M-H]-) | 7.55 | 7.53 |
| LMFA03010022 | 13,14-dihydro-15-keto-PGD2 | 11,15-dioxo-9S-hydroxy-5Z-prostenoic acid | 351([M-H]-) | 7.75 | 9.08 |
| LMFA03010035 | PGA2 | 9-oxo-15S-hydroxy-5Z,10Z,13E-prostatrienoic acid | 333([M-H]-) | 8.22 | 9.75 |
| LMFA03010019 | PGJ2 | 11-oxo-15S-hydroxy-5Z,9,13E-prostatrienoic acid | 333([M-H]-) | 8.39 | 9.95 |
| LMFA03010018 | PGB2 | 15S-hydroxy-9-oxo-5Z,8(12),13E-prostatrienoic acid | 333([M-H]-) | 8.42 | 9.82 |
| LMFA03010033 | 13,14-dihydro-15 keto-PGA2 | 9,15-dioxo-5Z,10-prostadienoic acid | 333([M-H]-) | 9.12 | 10.32 |
| LMFA03060010 | 5S,15S-DiHETE | 5S,15S-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid | 335([M-H]-) | 9.2 | 11.03 |
| LMFA03050010 | (+/-)14,15-DiHETrE | 14,15-dihydroxy-5Z,8Z,11Z-eicosatrienoic acid | 337([M-H]-) | 9.67 | 12.7 |
| LMFA03050008 | (+/-)11,12-DiHETrE | 11,12-dihydroxy-5Z,8Z,14Z-eicosatrienoic acid | 337([M-H]-) | 9.84 | 13.01 |
| LMFA03010021 | 15-deoxy-δ-12,14-PGJ2 | 11-oxo-5Z,9,12E,14E-prostatetraenoic acid | 315([M-H]-) | 9.98 | 13.01 |
| LMFA03050006 | (+/-)8,9-DiHETrE | 8,9-dihydroxy-5Z,11Z,14Z-eicosatrienoic acid | 337([M-H]-) | 9.99 | 13.4 |
| LMFA03060018 | 5S,6S-DiHETE | 5S,6S-dihydroxy-7E,9E,11Z,14Z-eicosatetraenoic acid | 335([M-H]-) | 10.2 | 13.8 |
| LMFA03050004 | (+/-)5,6-DiHETrE | 5,6-dihydroxy-8Z,11Z,14Z-eicosatrienoic acid | 337([M-H]-) | 10.25 | 14.04 |
| LMFA03020003 | LTC4 | 5S-hydroxy-6R-(S-glutathionyl),7E,9E,11Z,14Z-eicosatetraenoic acid | 624([M-H]-) | 10.25 | 12.99 |
| LMFA03060001 | 15S-HETE | 15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid | 319([M-H]-) | 10.45 | 14.33 |
| LMFA03060014 | 15S-HpETE | 15S-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid | 335([M-H]-) | 10.5 | 14.48 |
| LMFA03060003 | 11S-HETE | 11S-hydroxy-5Z,8Z,12E,14Z-eicosatetraenoic acid | 319([M-H]-) | 10.62 | 14.83 |
| LMFA03060019 | 12-oxo-ETE | 12-oxo-5Z,8Z,10E,14Z-eicosatetraenoic acid | 317([M-H]-) | 10.67 | 14.59 |
| LMFA03060008 | 12R-HETE | 12R-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid | 319([M-H]-) | 10.7 | 14.77 |
| LMFA03060007 | 12S-HETE | 12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid | 319([M-H]-) | 10.7 | 14.8 |
| LMFA03060006 | 8S-HETE | 8S-hydroxy-5Z,9E,11Z,14Z-eicosatetraenoic acid | 319([M-H]-) | 10.76 | 14.86 |
| LMFA03060002 | 5S-HETE | 5S-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid | 319([M-H]-) | 11.06 | 15.36 |
| LMFA03060012 | 5S-HpETE | 5S-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid | 335([M-H]-) | 11.08 | 15.42 |
| LMFA03060011 | 5-Oxo-ETE | 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid | 317([M-H]-) | 11.27 | 15.51 |
| LMFA03080004 | (+/-)11,12-EpETrE | 11,12-epoxy-5Z,8Z,14Z-eicosatrienoic acid | 319([M-H]-) | 11.36 | 15.83 |
| LMFA03080003 | (+/-)8,9-EpETrE | 8,9-epoxy-5Z,11Z,14Z-eicosatrienoic acid | 319([M-H]-) | 11.5 | 16.05 |
| LMFA03080002 | (+/-)5,6-EpETrE | 5,6-epoxy-8Z,11Z,14Z-eicosatrienoic acid | 319([M-H]-) | 11.7 | 16.3 |
| LMFA01030001 | Arachidonic acid | 5Z,8Z,11Z,14Z-eicosatetraenoic acid | 303([M-H]-) | 14.37 | 18.97 |