Experimental data
LIPID MAPS NASH study
We used an "omics" approach — profiling liver biopsies, plasma, and urine samples — to detect a reproducible signature of lipid metabolites, aqueous intracellular metabolites, SNPs, and mRNA transcripts in a double-blinded study of patients with different stages of nonalcoholic fatty liver disease (NAFLD). Using linear discriminant analysis, we identified a panel of 20 plasma metabolites that can be used to differentiate between NASH and steatosis. This identification of differential biomolecular signatures has the potential to improve clinical diagnosis and facilitate therapeutic intervention of NAFLD.
Reference: Biomarkers of NAFLD progression: a lipidomics approach to an epidemic. J Lipid Res. 2015 Mar;56(3):722-36. doi: 10.1194/jlr.P056002. Epub 2015 Jan 17.
Lipidomics studies on human plasma
Centralized studies on macrophages
We performed both lipidomics studies and microarray analysis of RAW 264.7 cells and primary macrophages, using various treatments. These studies were conducted using standardized protocols for isolation, expansion and analysis of primary macrophages and macrophage cell lines. Central preparation of cells enable LIPID MAPS lipidomics cores to characterize lipids under the most uniform conditions possible and allow results in one core unit to be directly compared to results in other core units.
Lipidomics studies on macrophages
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Primary macrophages
- Thioglycolate-elicited peritoneal macrophages (TGEM)
- Bone marrow-derived macrophages (BMDM)
- Compare results from TGEM and BMDM treated with Kdo2-Lipid A (3 time points: 0,6,24 hrs)
- Compare results from RAW, TGEM, and BMDM for sterols treated with Kdo2-Lipid A (full time course: 8 time points)
Microarray analysis of macrophages
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RAW 264.7 cells
- Kdo2-Lipid A time course experiments
- Compactin/Kdo2-Lipid A time course experiments (2 time points: 12, 24hrs)
- Compactin/Kdo2-Lipid A time course experiments (full time course: 7 time points)
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Primary macrophages
- Thioglycolate-elicited peritoneal macrophages (TGEM)
- Bone marrow-derived macrophages (BMDM)
Subcellular fractionation studies on macrophages<
Measurements of lipid quanitities present in various cell fractions (cytoplasm, ER, mitochondria, nucleus, plasma membrane) for control and treatment with Kdo2-Lipid A
- RAW 264.7 cells